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Team:Paris/Freezer Digestion Products
From 2009.igem.org
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| Line 213: | Line 213: | ||
| SpeI | | SpeI | ||
| 95pb | | 95pb | ||
| + | | Yes | ||
| + | | ??? | ||
| + | | [https://2009.igem.org/Team:Paris/11_August_2009 /11/08] | ||
| + | |- style="background: #ccccff; text-align: center;" | ||
| + | |style="background:#FFFF00"| D16 | ||
| + | | pLac | ||
| + | | [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P21 P21]]BBa-I1014 | ||
| + | | P21 | ||
| + | | EcoRI | ||
| + | | XbaI | ||
| + | | ??? | ||
| Yes | | Yes | ||
| ??? | | ??? | ||
| [https://2009.igem.org/Team:Paris/11_August_2009 /11/08] | | [https://2009.igem.org/Team:Paris/11_August_2009 /11/08] | ||
|} | |} | ||
Revision as of 15:31, 12 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Digestion Products
Red = Vector
Yellow = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Description | Matrix ID | Enz. 1 | Enz. 2 | Size (bp) | Digested? | Purified? | [ng/µL] |
| D1 | P3 | [P3]BBa-J32015 | XbaI | PstI | 66pb | PelB leader sequence | 47 | ??? | 08/04 |
| D2 | P4 | [P4]BBa-B0014 | XbaI | PstI | 95pb | Double terminator bidirectionnal (B0012-B0011) | 47 | ??? | 08/04 |
| D3 | P5 | [P5]BBa-I14032 | XbaI | PstI | 37pb | pLac | 47 | ??? | 08/04 |
| D4 | P7 | [P7]Bba-R0040 | XbaI | PstI | 54pb | TetR repressible promote | 47 | ??? | 08/04 |
| D5 | P8 | [P8]BBa-I0500 | XbaI | PstI | 1210pb | pBad/araC | 47 | ??? | 08/04 |
| D6 | P2 | [P2] BBa-J61002 | XbaI | PstI | 2053bp (and 895bp) | Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). | 47 | ??? | 08/04 |
| D7 | pSB2K3 | ??? | P15 | XbaI | PstI | Size | Yes | Yes | 08/07 |
| D8 | pSB1A3 | ??? | P16 | XbaI | PstI | Size | Yes | Yes | 08/07 |
| D9 | plbb RFP | ??? | P17 | EcoRI | SpeI | Size | Yes | Yes | 08/07 |
| D10 | ptet | ??? | P18 | XbaI | PstI | Size | Yes | Yes | 08/07 |
| D11 | RBS-tet | ??? | P19 | XbaI | PstI | Size | Yes | Yes | 08/07 |
| D12 | pLac | In order to open the vector to add RBS-tet (XbaI/PstI) | P21 | SpeI | PstI | Size | Yes | Yes | 08/10 |
| D13 | OmpAsignal | ??? | A11 | XbaI | PstI | ~135 | Yes | Yes | 11/07 |
| D14 | TolRII | ??? | A13 | XbaI | PstI | ~279 | Yes | Yes | 11/07 |
| D15 | double terminator | [P4]BBa-B0014 | P4 | EcoRI | SpeI | 95pb | Yes | ??? | /11/08 |
| D16 | pLac | [P21]BBa-I1014 | P21 | EcoRI | XbaI | ??? | Yes | ??? | /11/08 |
