Team:Paris/Freezer Plasmids

From 2009.igem.org

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|width=5%| P26 <span/ id="P26">
|width=5%| P26 <span/ id="P26">
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|- style="background: #ccccff; text-align: center;"
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|width=5%| P27 <span/ id="P27">
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|width=5%| P28 <span/ id="P28">
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|width=25%| [http://partsregistry.org/Part:BBa_K103006 BBa_K103006]
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|width=50%| OmpA linker (464bp)
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|width=15%| PCR @57°C+ 15s@72°C
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|width=5%| [https://2009.igem.org/Team:Paris/14_August_2009 08/14]
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Revision as of 15:08, 14 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Plasmids

Plasmids: MiniPrep Products
Number Name Description Products in use Date
P1 [http://partsregistry.org/Part:pSB2K3 pSB2K3] General plasmid for cloning step after PCR Products in use 08/03
P2 [http://partsregistry.org/Part:BBa_J61002 Bba_J61002] Plasmid backbone, it allows us to change promoter RFP as promoter Products in use 08/03
P3 [http://partsregistry.org/Part:BBa_J32015 Bba_J32015] PelB leader sequence Products in use 08/03
P4 [http://partsregistry.org/Part:BBa_B0014 Bba_B0014] Double terminator bidirectionnal (B0012-B0011) Products in use 08/03
P5 [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] pLac Products in use 08/03
P6 [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] OmpA linker (464bp) Products in use 08/03
P7 [http://partsregistry.org/Part:BBa_R0040 Bba_R0040] TetR repressible promoter Products in use 08/03
P8 [http://partsregistry.org/Part:BBa_I0500 Bba_I0500] pBad/araC Products in use 08/03
P9 ΔN-Syn1A  ??? Products in use 08/03
P10 His6-SNAP25  ??? Products in use 08/03
P11 VAMP2-HiB6  ??? Products in use 08/03
P12 g3p g3p in pHEN2 plasmid Products in use 08/04
P13 [http://partsregistry.org/Part:pSB1A3 pSB1A3] High copy BioBrick assembly plasmid Products in use 08/06
P14 [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] RBS-tetR Products in use 08/06
P15 [http://partsregistry.org/Part:pSB2K3 pSB2K3] plasmid backbone All have been digested and purified 08/07
P16 [http://partsregistry.org/Part:pSB1A3 pSB1A3] plasmid backbone All have been digested and purified 08/07
P17 [http://partsregistry.org/Part:BBa_J61002 BBa_J61002] plasmid backbone with RFP All have been digested and purified 08/07
P18 [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] ptet All have been digested and purified 08/07
P19 [http://partsregistry.org/Part:BBa_K136050 BBa_K136050] RBS-tet All have been digested and purified 08/07
P20 [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] RBS-lacI ... 08/08
P21 [http://partsregistry.org/Part:BBa_I14032 Bba_I14032] pLac Products in use 08/10
P22
P23
P24
P25
P26
P27
P28 [http://partsregistry.org/Part:BBa_K103006 BBa_K103006] OmpA linker (464bp) PCR @57°C+ 15s@72°C 08/14

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