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Team:Southampton/Project/Protocols
From 2009.igem.org
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<h3>As part of the preparation for iGEM 2009, we have peformed a number of experimental processes which are detailed in protocols. We are making these protocols available on our wiki to help future teams in their synthetic approach. These protocols can be accesed together with an explanation of the theoretical basis using the links embedded in the diagram below:</h3> | <h3>As part of the preparation for iGEM 2009, we have peformed a number of experimental processes which are detailed in protocols. We are making these protocols available on our wiki to help future teams in their synthetic approach. These protocols can be accesed together with an explanation of the theoretical basis using the links embedded in the diagram below:</h3> | ||
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<img id="Untitled_1_01" src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_01.gif" width="822" height="78" alt="" /></td> | <img id="Untitled_1_01" src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_01.gif" width="822" height="78" alt="" /></td> | ||
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<img src="http://www.personal.soton.ac.uk/jeh2v07/images/spacer.gif" width="1" height="78" alt="" /></td> | <img src="http://www.personal.soton.ac.uk/jeh2v07/images/spacer.gif" width="1" height="78" alt="" /></td> | ||
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<img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_02.gif" alt="" width="45" height="905" border="0" id="Untitled_1_02" /></td> | <img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_02.gif" alt="" width="45" height="905" border="0" id="Untitled_1_02" /></td> | ||
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<a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/trans.gif" width="338" height="84" rel="lightbox[pro]" title="Transformation - Single plasmids are introduced into recipient bacterial cells. The cells are allowed to replicate in order to achieve high yields of plasmid DNA."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_03.gif" width="338" height="84" border="0" /></a></td> | <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/trans.gif" width="338" height="84" rel="lightbox[pro]" title="Transformation - Single plasmids are introduced into recipient bacterial cells. The cells are allowed to replicate in order to achieve high yields of plasmid DNA."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_03.gif" width="338" height="84" border="0" /></a></td> | ||
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| - | <td><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#transformation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_05.gif" alt="" name="Untitled_1_05" width="162" height="32" border="0" id="Untitled_1_05" /></a></td> | + | <td width="162"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#transformation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_05.gif" alt="" name="Untitled_1_05" width="162" height="32" border="0" id="Untitled_1_05" /></a></td> |
<td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_06.gif" alt="" name="Untitled_1_06" width="176" height="32" border="0" id="Untitled_1_06" /></a></td> | <td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_06.gif" alt="" name="Untitled_1_06" width="176" height="32" border="0" id="Untitled_1_06" /></a></td> | ||
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<td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#plasmidprep"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_09.gif" alt="" name="Untitled_1_09" width="171" height="24" border="0" id="Untitled_1_09" /></a></td> | <td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#plasmidprep"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_09.gif" alt="" name="Untitled_1_09" width="171" height="24" border="0" id="Untitled_1_09" /></a></td> | ||
| - | <td rowspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_10.gif" alt="" name="Untitled_1_10" width="167" height="30" border="0" id="Untitled_1_10" /></a></td> | + | <td width="167" rowspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_10.gif" alt="" name="Untitled_1_10" width="167" height="30" border="0" id="Untitled_1_10" /></a></td> |
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<img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_14.gif" alt="" name="Untitled_1_14" width="75" height="498" border="0" id="Untitled_1_14" /></td> | <img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_14.gif" alt="" name="Untitled_1_14" width="75" height="498" border="0" id="Untitled_1_14" /></td> | ||
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<a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/gel.gif" width="186" height="174" rel="lightbox[pro]" title="Gel Electrophoresis - Distance travelled by the DNA helps identify the DNA molecules present."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_15.gif" width="186" height="174" border="0" /></td> | <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/gel.gif" width="186" height="174" rel="lightbox[pro]" title="Gel Electrophoresis - Distance travelled by the DNA helps identify the DNA molecules present."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_15.gif" width="186" height="174" border="0" /></td> | ||
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<img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_16.gif" alt="" name="Untitled_1_16" width="178" height="85" border="0" id="Untitled_1_16" /></td> | <img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_16.gif" alt="" name="Untitled_1_16" width="178" height="85" border="0" id="Untitled_1_16" /></td> | ||
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| - | <td rowspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_20.gif" alt="" name="Untitled_1_20" width="143" height="38" border="0" id="Untitled_1_20" /></a></td> | + | <td width="143" rowspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_20.gif" alt="" name="Untitled_1_20" width="143" height="38" border="0" id="Untitled_1_20" /></a></td> |
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Revision as of 08:55, 16 October 2009
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Protocol Map
As part of the preparation for iGEM 2009, we have peformed a number of experimental processes which are detailed in protocols. We are making these protocols available on our wiki to help future teams in their synthetic approach. These protocols can be accesed together with an explanation of the theoretical basis using the links embedded in the diagram below:
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