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Team:Southampton/Project/Protocols
From 2009.igem.org
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<a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/trans.gif" width="338" height="84" rel="lightbox[pro]" title="Transformation - Single plasmids are introduced into recipient bacterial cells. The cells are allowed to replicate in order to achieve high yields of plasmid DNA."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_03.gif" width="338" height="84" border="0" /></a></td> | <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/trans.gif" width="338" height="84" rel="lightbox[pro]" title="Transformation - Single plasmids are introduced into recipient bacterial cells. The cells are allowed to replicate in order to achieve high yields of plasmid DNA."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_03.gif" width="338" height="84" border="0" /></a></td> | ||
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| - | <td width="162"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#transformation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_05.gif" alt="" name="Untitled_1_05" width="162" height="32" border="0" id="Untitled_1_05" /></a></td> | + | <td width="162" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#transformation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_05.gif" alt="" name="Untitled_1_05" width="162" height="32" border="0" id="Untitled_1_05" /></a></td> |
| - | <td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_06.gif" alt="" name="Untitled_1_06" width="176" height="32" border="0" id="Untitled_1_06" /></a></td> | + | <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_06.gif" alt="" name="Untitled_1_06" width="176" height="32" border="0" id="Untitled_1_06" /></a></td> |
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<a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/plasmidprep.gif" width="338" height="84" rel="lightbox[pro]" title="Pureyield Plasmid Preparation - A culture of bacterial cells are grown up. The plasmids are then extracted from the cells."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_08.gif" width="338" height="87" border="0" /></a></td> | <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/plasmidprep.gif" width="338" height="84" rel="lightbox[pro]" title="Pureyield Plasmid Preparation - A culture of bacterial cells are grown up. The plasmids are then extracted from the cells."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_08.gif" width="338" height="87" border="0" /></a></td> | ||
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| - | <td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#plasmidprep"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_09.gif" alt="" name="Untitled_1_09" width="171" height="24" border="0" id="Untitled_1_09" /></a></td> | + | <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#plasmidprep"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_09.gif" alt="" name="Untitled_1_09" width="171" height="24" border="0" id="Untitled_1_09" /></a></td> |
| - | <td width="167" rowspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_10.gif" alt="" name="Untitled_1_10" width="167" height="30" border="0" id="Untitled_1_10" /></a></td> | + | <td width="167" rowspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_10.gif" alt="" name="Untitled_1_10" width="167" height="30" border="0" id="Untitled_1_10" /></a></td> |
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<a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/pcr.gif" width="338" height="84" rel="lightbox[pro]" title="Polymerase Chain Reaction - DNA replication is instigated to increase DNA concentration. (Invitrogen Platinum® Pfx)"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_13.gif" width="338" height="84" border="0" /></td> | <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/pcr.gif" width="338" height="84" rel="lightbox[pro]" title="Polymerase Chain Reaction - DNA replication is instigated to increase DNA concentration. (Invitrogen Platinum® Pfx)"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_13.gif" width="338" height="84" border="0" /></td> | ||
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<a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/gel.gif" width="186" height="174" rel="lightbox[pro]" title="Gel Electrophoresis - Distance travelled by the DNA helps identify the DNA molecules present."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_15.gif" width="186" height="174" border="0" /></td> | <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/gel.gif" width="186" height="174" rel="lightbox[pro]" title="Gel Electrophoresis - Distance travelled by the DNA helps identify the DNA molecules present."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_15.gif" width="186" height="174" border="0" /></td> | ||
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| - | <td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#pcr"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_17.gif" alt="" name="Untitled_1_17" width="171" height="35" border="0" id="Untitled_1_17" /></a></td> | + | <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#pcr"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_17.gif" alt="" name="Untitled_1_17" width="171" height="35" border="0" id="Untitled_1_17" /></a></td> |
| - | <td><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Hfpcr"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_18.gif" alt="" name="Untitled_1_18" width="167" height="35" border="0" id="Untitled_1_18" /></a></td> | + | <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Hfpcr"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_18.gif" alt="" name="Untitled_1_18" width="167" height="35" border="0" id="Untitled_1_18" /></a></td> |
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| - | <td width="143" rowspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_20.gif" alt="" name="Untitled_1_20" width="143" height="38" border="0" id="Untitled_1_20" /></a></td> | + | <td width="143" rowspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_20.gif" alt="" name="Untitled_1_20" width="143" height="38" border="0" id="Untitled_1_20" /></a></td> |
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<img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_21.gif" alt="" name="Untitled_1_21" width="35" height="413" border="0" id="Untitled_1_21" /></td> | <img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_21.gif" alt="" name="Untitled_1_21" width="35" height="413" border="0" id="Untitled_1_21" /></td> | ||
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<a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/digest.gif" width="338" height="84" rel="lightbox[pro]" title="Digestion - DNA strands are cut to either remove a sequence of base pairs or open up a plasmid."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_22.gif" width="338" height="150" border="0" /></td> | <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/digest.gif" width="338" height="84" rel="lightbox[pro]" title="Digestion - DNA strands are cut to either remove a sequence of base pairs or open up a plasmid."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_22.gif" width="338" height="150" border="0" /></td> | ||
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| - | <td><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_23.gif" alt="" name="Untitled_1_23" width="143" height="51" border="0" id="Untitled_1_23" /></a></td> | + | <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_23.gif" alt="" name="Untitled_1_23" width="143" height="51" border="0" id="Untitled_1_23" /></a></td> |
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<img src="http://www.personal.soton.ac.uk/jeh2v07/images/spacer.gif" width="1" height="51" alt="" /></td> | <img src="http://www.personal.soton.ac.uk/jeh2v07/images/spacer.gif" width="1" height="51" alt="" /></td> | ||
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| - | <td><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#digestion"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_25.gif" alt="" name="Untitled_1_25" width="162" height="40" border="0" id="Untitled_1_25" /></a></td> | + | <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#digestion"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_25.gif" alt="" name="Untitled_1_25" width="162" height="40" border="0" id="Untitled_1_25" /></a></td> |
| - | <td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/DoubleDigestion"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_26.gif" alt="" name="Untitled_1_26" width="176" height="40" border="0" id="Untitled_1_26" /></a></td> | + | <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/DoubleDigestion"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_26.gif" alt="" name="Untitled_1_26" width="176" height="40" border="0" id="Untitled_1_26" /></a></td> |
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<a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/ligate.gif" width="338" height="88" rel="lightbox[pro]" title="Ligation - Two samples of DNA are joined together."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_28.gif" width="338" height="88" border="0" /></td> | <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/ligate.gif" width="338" height="88" rel="lightbox[pro]" title="Ligation - Two samples of DNA are joined together."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_28.gif" width="338" height="88" border="0" /></td> | ||
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| - | <td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#ligation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_29.gif" alt="" name="Untitled_1_29" width="171" height="36" border="0" id="Untitled_1_29" /></a></td> | + | <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#ligation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_29.gif" alt="" name="Untitled_1_29" width="171" height="36" border="0" id="Untitled_1_29" /></a></td> |
| - | <td><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_30.gif" alt="" name="Untitled_1_30" width="167" height="36" border="0" id="Untitled_1_30" /></a></td> | + | <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_30.gif" alt="" name="Untitled_1_30" width="167" height="36" border="0" id="Untitled_1_30" /></a></td> |
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Revision as of 08:55, 16 October 2009
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Protocol Map
As part of the preparation for iGEM 2009, we have peformed a number of experimental processes which are detailed in protocols. We are making these protocols available on our wiki to help future teams in their synthetic approach. These protocols can be accesed together with an explanation of the theoretical basis using the links embedded in the diagram below:
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