Team:Paris/Freezer PCR products

From 2009.igem.org

(Difference between revisions)
(→PCR products)
(→PCR products)
Line 146: Line 146:
|width=5%| ?
|width=5%| ?
|width=5%| ?
|width=5%| ?
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A9 <span/ id="A8">
 +
|width=10%| PFec A
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O29 O29 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O30 O30 ]
 +
|width=5%| (65.1)
 +
|width=10%| ?
 +
|width=10%| 163
 +
|width=25%| For signal transduction
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009#PCR 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A10 <span/ id="A10">
 +
|width=10%| clyA_fusion C term
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O31 O31 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O10 O10 ]
 +
|width=5%| ?(55.5)
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
 +
|width=10%| 987
 +
|width=25%| Amplification of clyA with RBS in the primer
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| ?
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A11 <span/ id="A11">
 +
|width=10%| OmpAsignal_fusion C term
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O11 O11 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O12 O12 ]
 +
|width=5%| ?(70.4)
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
 +
|width=10%| 135
 +
|width=25%| Amplification of OmpA signal for export to periplasm
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| ?
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A12 <span/ id="A12">
 +
|width=10%| Tg3p_fusion N term
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O18 O18 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O17 O17 ]
 +
|width=5%| ? (63.5)
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
 +
|width=10%| 274
 +
|width=25%| Amplification of N-term domain of M13's g3p
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009#PCR 08/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A13 <span/ id="A13">
 +
|width=10%| tolRII_fusion N term
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O13 O13 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O1 O1 ]
 +
|width=5%| (73.5)
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
 +
|width=10%| 279
 +
|width=25%| Amplification of tolR
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| ?
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| A14 <span/ id="A14">
 +
|width=10%| tatABC_RBS
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O14 O14 ] 
 +
|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O15 O15 ]
 +
|width=5%| (63.5)
 +
|width=10%| [https://2009.igem.org/Team:Paris/Fridge F4]
 +
|width=10%| 1634
 +
|width=25%| Amplification of tatA with RBS
 +
|width=5%| ?
 +
|width=5%| ?
 +
|width=5%| [https://2009.igem.org/Team:Paris/7_August_2009#PCR 08/07]
|}
|}
-
 
<br>
<br>
<br>
<br>

Revision as of 09:49, 10 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

PCR products

PCR products
Ref Name Oligo Fw Oligo Rev Tm used(Tm cal.) (°C) Matrix Length (bp) Comments Quantity [ ] (ng/µL) Date
A1 Tg3p_fusion N term O18 O17  ? (63.5) F4 274 Amplification of N-term domain of M13's g3p  ?  ? 08/05
A2 TE3_fusion N term O15 O16  ?  ??? 1036 Amplification of N-term domain of colicin E3  ?  ? 08/05
A3 clyA_fusion C term O31 O10  ?(55.5) F4 987 Amplification of clyA with RBS in the primer  ?  ? 08/05
A4 OmpAsignal_fusion C term O11 O12  ?(70.4) F4 135 Amplification of OmpA signal for export to periplasm  ?  ? 08/05
A5 tolRII_fusion N term O13 O1 (73.5) F4 279 Amplification of tolR  ?  ? 08/05
A6 tatABC_RBS O14 O15 (63.5) F4 1634 Amplification of tatA with RBS  ?  ? 08/05
A7 g3p_std O19 O20 (52) P12 1287 For standardisation of g3p and adding SacI site  ?  ? 08/08
A8 OmpA_L_std O21 O22 (59.5) P6 525 For standardisation of OmpA linker (BBaK103006)  ?  ?  ?
A9 PFec A O29 O30 (65.1)  ? 163 For signal transduction  ?  ? 08/07
A10 clyA_fusion C term O31 O10  ?(55.5) F4 987 Amplification of clyA with RBS in the primer  ?  ?  ?
A11 OmpAsignal_fusion C term O11 O12  ?(70.4) F4 135 Amplification of OmpA signal for export to periplasm  ?  ?  ?
A12 Tg3p_fusion N term O18 O17  ? (63.5) F4 274 Amplification of N-term domain of M13's g3p  ?  ? 08/07
A13 tolRII_fusion N term O13 O1 (73.5) F4 279 Amplification of tolR  ?  ?  ?
A14 tatABC_RBS O14 O15 (63.5) F4 1634 Amplification of tatA with RBS  ?  ? 08/07



http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png http://parts.mit.edu/images/partbypart/icon_regulatory.png http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_PCR_products"