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Team:Paris/Freezer Digestion Products
From 2009.igem.org
(Difference between revisions)
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| PstI | | PstI | ||
| Size | | Size | ||
| - | | | + | | pSB2K3 |
| ??? | | ??? | ||
| ??? | | ??? | ||
| Line 135: | Line 135: | ||
| PstI | | PstI | ||
| Size | | Size | ||
| - | | | + | | pSB1A3 |
| ??? | | ??? | ||
| ??? | | ??? | ||
| Line 146: | Line 146: | ||
| SpeI | | SpeI | ||
| Size | | Size | ||
| - | | | + | | plbb RFP |
| ??? | | ??? | ||
| ??? | | ??? | ||
| Line 157: | Line 157: | ||
| PstI | | PstI | ||
| Size | | Size | ||
| - | | | + | | ptet |
| ??? | | ??? | ||
| ??? | | ??? | ||
| Line 168: | Line 168: | ||
| PstI | | PstI | ||
| Size | | Size | ||
| - | | | + | | RBS-tet |
| ??? | | ??? | ||
| ??? | | ??? | ||
Revision as of 20:03, 10 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
Digestion Products
Red = Vector
Yellow = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Name | Description | Matrix ID | Enz. 1 | Enz. 2 | Size (bp) | Digested? | Purified? | [ng/µL] |
| D1 | P3 | [P3]BBa-J32015 | XbaI | PstI | 66pb | PelB leader sequence | 47 | ??? | 08/04 |
| D2 | P4 | [P4]BBa-B0014 | XbaI | PstI | 95pb | Double terminator bidirectionnal (B0012-B0011) | 47 | ??? | 08/04 |
| D3 | P5 | [P5]BBa-I14032 | XbaI | PstI | 37pb | pLac | 47 | ??? | 08/04 |
| D4 | P7 | [P7]Bba-R0040 | XbaI | PstI | 54pb | TetR repressible promote | 47 | ??? | 08/04 |
| D5 | P8 | [P8]BBa-I0500 | XbaI | PstI | 1210pb | pBad/araC | 47 | ??? | 08/04 |
| D6 | P2 | [P2] BBa-J61002 | XbaI | PstI | 2053bp (and 895bp) | Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). | 47 | ??? | 08/04 |
| D7 | P15 | ??? | XbaI | PstI | Size | pSB2K3 | ??? | ??? | 08/07 |
| D8 | P16 | ??? | XbaI | PstI | Size | pSB1A3 | ??? | ??? | 08/07 |
| D9 | P17 | ??? | EcoRI | SpeI | Size | plbb RFP | ??? | ??? | 08/07 |
| D10 | P18 | ??? | XbaI | PstI | Size | ptet | ??? | ??? | 08/07 |
| D11 | P19 | ??? | XbaI | PstI | Size | RBS-tet | ??? | ??? | 08/07 |
| D12 | P21 | ??? | SpeI | PstI | Size | ??? | ??? | ??? | 08/07 |
