Team:EPF-Lausanne/Notebook

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=Notebook=
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===06.07.09===
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<font size="12" color="#007CBC">Notebook</font>
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;Wet lab:
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LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli following IGEM protocol, and grown overnight (see Lab book for more details).
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<br>
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<br>One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl.
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<br>
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LOVTAP is in a plasmid called pCal-n (see picture below):
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[[Image: pCAL-n.jpg|500px|thumb|center|pCal-n plasmid]]
 
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<br>Some comments on the plasmid:
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==Calendar==
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<br>-CBP is a small peptide with which we could purify LOVTAP protein
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<br>-Thrombin target is a nucleotidic sequence that can be recognized by thrombin a peptidase. This peptidase will cut CBP once LOVTAP is purified
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;Cloning strategy:
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<br>Four forward primers were designed to amplify:
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<br> 1.Promoter T7, RBS, CBP and LOVTAP:  
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<td valign=top>{{ #calendar: title=EPF-Lausanne |year=2009 | month=06 }}
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:gtttcttcgaattcgcggccgcttctagagtaatacgactcactataggggaattgtg
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2.RBS, CBP and LOVTAP:
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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:gtttcttcgaattcgcggccgcttctagagtgtttaactttaagaaggag
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3.CBP and LOVTAP:  
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{{ #calendar: title=EPF-Lausanne |year=2009 | month=07 }}
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:gtttcttcgaattcgcggccgcttctagatgaagcgacgatggaaaaagaatttcatag
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4.LOVTAP:
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:gtttcttcgaattcgcggccgcttctagatgctactacacttgaacgtattgagaagaac
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{{ #calendar: title=EPF-Lausanne |year=2009 | month=08 }}
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One reverse primer were designed:  
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{{ #calendar: title=EPF-Lausanne |year=2009 | month=09 }}
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{{ #calendar: title=EPF-Lausanne |year=2009 | month=10 }}
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<br>The <b>recipient IGEM backbone</b> have been chosen: [http://partsregistry.org/Part:pSB3C5 pSB3C5], well 5G in the received kit plate 1
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!align="center"|[[Team:EPF-Lausanne|Home]]
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!align="center"|[[Team:EPF-Lausanne/Team|The Team]]
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!align="center"|[[Team:EPF-Lausanne/Project|The Project]]
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!align="center"|[[Team:EPF-Lausanne/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:EPF-Lausanne/Modeling|Modeling]]
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!align="center"|[[Team:EPF-Lausanne/Notebook|Notebook]]
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!align="center"|[[Team:EPF-Lausanne/Lectures|Lectures]]
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!align="center"|[[Team:EPF-Lausanne/Team Management|Team Management]]
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!align="center"|[[Team:EPF-Lausanne/References|References]]
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Latest revision as of 18:24, 20 October 2009



Contents








Notebook


27.04.2024 | 20:59


Calendar

           
June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
Retrieved from "http://2009.igem.org/Team:EPF-Lausanne/Notebook"