Team:Paris/Freezer Digestion Products

From 2009.igem.org

(Difference between revisions)
(→Digestion Products)
(→Digestion Products)
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|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|style="background:#FFFF00"| D7
|style="background:#FFFF00"| D7
-
| P15
+
| pSB2K3
| ???
| ???
 +
| P15
| XbaI
| XbaI
| PstI
| PstI
| Size
| Size
-
| pSB2K3
 
| ???
| ???
| ???
| ???
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|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|style="background:#FFFF00"| D8
|style="background:#FFFF00"| D8
-
| P16
+
| pSB1A3
| ???
| ???
 +
| P16
| XbaI
| XbaI
| PstI
| PstI
| Size
| Size
-
| pSB1A3
 
| ???
| ???
| ???
| ???
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|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|style="background:#FFFF00"| D9
|style="background:#FFFF00"| D9
-
| P17
+
| plbb RFP
| ???
| ???
 +
| P17
| EcoRI
| EcoRI
| SpeI
| SpeI
| Size
| Size
-
| plbb RFP
 
| ???
| ???
| ???
| ???
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|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|style="background:#FFFF00"| D10
|style="background:#FFFF00"| D10
 +
| ptet
 +
| ???
| P18
| P18
-
| ???
 
| XbaI
| XbaI
| PstI
| PstI
| Size
| Size
-
| ptet
 
| ???
| ???
| ???
| ???
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|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|style="background:#FFFF00"| D11
|style="background:#FFFF00"| D11
-
| P19
+
| RBS-tet
| ???
| ???
 +
| P19
| XbaI
| XbaI
| PstI
| PstI
| Size
| Size
-
| RBS-tet
+
| Yes
-
| ???
+
| Yes
-
| ???
+
| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
|- style="background: #ccccff; text-align: center;"
|- style="background: #ccccff; text-align: center;"
|style="background:#FFFF00"| D12
|style="background:#FFFF00"| D12
 +
| pLac
 +
| In order to open the vector to add RBS-tet (XbaI/PstI)
| P21
| P21
-
| ???
 
| SpeI
| SpeI
| PstI
| PstI
| Size
| Size
-
| ???
+
| Yes
-
| ???
+
| No
-
| ???
+
| [https://2009.igem.org/Team:Paris/10_August_2009 08/10]
-
| [https://2009.igem.org/Team:Paris/7_August_2009 08/07]
+
|}
|}

Revision as of 20:11, 10 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Digestion Products

Red = Vector
Yellow = Insert
Digestion Products
Number Name Description Matrix ID Enz. 1 Enz. 2 Size (bp) Digested? Purified? [ng/µL]
D1 P3 [P3]BBa-J32015 XbaI PstI 66pb PelB leader sequence 47  ??? 08/04
D2 P4 [P4]BBa-B0014 XbaI PstI 95pb Double terminator bidirectionnal (B0012-B0011) 47  ??? 08/04
D3 P5 [P5]BBa-I14032 XbaI PstI 37pb pLac 47  ??? 08/04
D4 P7 [P7]Bba-R0040 XbaI PstI 54pb TetR repressible promote 47  ??? 08/04
D5 P8 [P8]BBa-I0500 XbaI PstI 1210pb pBad/araC 47  ??? 08/04
D6 P2 [P2] BBa-J61002 XbaI PstI 2053bp (and 895bp) Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). 47  ??? 08/04
D7 pSB2K3  ??? P15 XbaI PstI Size  ???  ??? 08/07
D8 pSB1A3  ??? P16 XbaI PstI Size  ???  ??? 08/07
D9 plbb RFP  ??? P17 EcoRI SpeI Size  ???  ??? 08/07
D10 ptet  ??? P18 XbaI PstI Size  ???  ??? 08/07
D11 RBS-tet  ??? P19 XbaI PstI Size Yes Yes 08/07
D12 pLac In order to open the vector to add RBS-tet (XbaI/PstI) P21 SpeI PstI Size Yes No 08/10

Retrieved from "http://2009.igem.org/Team:Paris/Freezer_Digestion_Products"