Team:Paris/Freezer Digestion Products

From 2009.igem.org

(Difference between revisions)
(Digestion Products)
(Digestion Products)
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| Yes
| Yes
| [https://2009.igem.org/Team:Paris/7_August_2009 11/07]
| [https://2009.igem.org/Team:Paris/7_August_2009 11/07]
 +
|- style="background: #ccccff; text-align: center;"
 +
|style="background:#FFFF00"| D15
 +
| double terminator
 +
| [[https://2009.igem.org/Team:Paris/Freezer_Plasmids#P4 P4]]BBa-B0014
 +
| P4
 +
| EcoRI
 +
| SpeI
 +
| 95pb
 +
| Yes
 +
| ???
 +
| [https://2009.igem.org/Team:Paris/11_August_2009 /11/08]
|}
|}

Revision as of 15:27, 12 August 2009

Freezer

Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock) - Construction in progress


Color code for sticker
Vector Insert PCR product Plasmid Strains Kanamycin Ampicilin Chloranphenicol

Digestion Products

Red = Vector
Yellow = Insert
Digestion Products
Number Name Description Matrix ID Enz. 1 Enz. 2 Size (bp) Digested? Purified? [ng/µL]
D1 P3 [P3]BBa-J32015 XbaI PstI 66pb PelB leader sequence 47  ??? 08/04
D2 P4 [P4]BBa-B0014 XbaI PstI 95pb Double terminator bidirectionnal (B0012-B0011) 47  ??? 08/04
D3 P5 [P5]BBa-I14032 XbaI PstI 37pb pLac 47  ??? 08/04
D4 P7 [P7]Bba-R0040 XbaI PstI 54pb TetR repressible promote 47  ??? 08/04
D5 P8 [P8]BBa-I0500 XbaI PstI 1210pb pBad/araC 47  ??? 08/04
D6 P2 [P2] BBa-J61002 XbaI PstI 2053bp (and 895bp) Plasmid backbone, it allows us to change promoter RFP as promoter. Purification of 2053bp band (vecter with pTet). 47  ??? 08/04
D7 pSB2K3  ??? P15 XbaI PstI Size Yes Yes 08/07
D8 pSB1A3  ??? P16 XbaI PstI Size Yes Yes 08/07
D9 plbb RFP  ??? P17 EcoRI SpeI Size Yes Yes 08/07
D10 ptet  ??? P18 XbaI PstI Size Yes Yes 08/07
D11 RBS-tet  ??? P19 XbaI PstI Size Yes Yes 08/07
D12 pLac In order to open the vector to add RBS-tet (XbaI/PstI) P21 SpeI PstI Size Yes Yes 08/10
D13 OmpAsignal  ??? A11 XbaI PstI ~135 Yes Yes 11/07
D14 TolRII  ??? A13 XbaI PstI ~279 Yes Yes 11/07
D15 double terminator [P4]BBa-B0014 P4 EcoRI SpeI 95pb Yes  ??? /11/08