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Team:Paris/Freezer PCR products
From 2009.igem.org
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O17 O17 ] | |width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O17 O17 ] | ||
|width=5%| ? (63.5) | |width=5%| ? (63.5) | ||
| - | |width=10%| | + | |width=10%| ? |
|width=10%| 274 | |width=10%| 274 | ||
|width=25%| Amplification of N-term domain of M13's g3p | |width=25%| Amplification of N-term domain of M13's g3p | ||
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|width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O17 O17 ] | |width=10%| [https://2009.igem.org/Team:Paris/Freezer_Primers#O17 O17 ] | ||
|width=5%| ? (63.5) | |width=5%| ? (63.5) | ||
| - | |width=10%| | + | |width=10%| ? |
|width=10%| 274 | |width=10%| 274 | ||
|width=25%| Amplification of N-term domain of M13's g3p | |width=25%| Amplification of N-term domain of M13's g3p | ||
Revision as of 09:51, 10 August 2009
Freezer
Primers - PCR products - Plasmids - Digestion Products - Unexpected digestion results - Strains (Glycerol stock)
| Color code for sticker | |||||||
|---|---|---|---|---|---|---|---|
| Vector | Insert | PCR product | Plasmid | Strains | Kanamycin | Ampicilin | Chloranphenicol |
PCR products
| PCR products | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ref | Name | Oligo Fw | Oligo Rev | Tm used(Tm cal.) (°C) | Matrix | Length (bp) | Comments | Quantity | [ ] (ng/µL) | Date |
| A1 | Tg3p_fusion N term | O18 | O17 | ? (63.5) | ? | 274 | Amplification of N-term domain of M13's g3p | ? | ? | 08/05 |
| A2 | TE3_fusion N term | O15 | O16 | ? | ??? | 1036 | Amplification of N-term domain of colicin E3 | ? | ? | 08/05 |
| A3 | clyA_fusion C term | O31 | O10 | ?(55.5) | F4 | 987 | Amplification of clyA with RBS in the primer | ? | ? | 08/05 |
| A4 | OmpAsignal_fusion C term | O11 | O12 | ?(70.4) | F4 | 135 | Amplification of OmpA signal for export to periplasm | ? | ? | 08/05 |
| A5 | tolRII_fusion N term | O13 | O1 | (73.5) | F4 | 279 | Amplification of tolR | ? | ? | 08/05 |
| A6 | tatABC_RBS | O14 | O15 | (63.5) | F4 | 1634 | Amplification of tatA with RBS | ? | ? | 08/05 |
| A7 | g3p_std | O19 | O20 | (52) | P12 | 1287 | For standardisation of g3p and adding SacI site | ? | ? | 08/08 |
| A8 | OmpA_L_std | O21 | O22 | (59.5) | P6 | 525 | For standardisation of OmpA linker (BBaK103006) | ? | ? | ? |
| A9 | PFec A | O29 | O30 | (65.1) | ? | 163 | For signal transduction | ? | ? | 08/07 |
| A10 | clyA_fusion C term | O31 | O10 | ?(55.5) | F4 | 987 | Amplification of clyA with RBS in the primer | ? | ? | ? |
| A11 | OmpAsignal_fusion C term | O11 | O12 | ?(70.4) | F4 | 135 | Amplification of OmpA signal for export to periplasm | ? | ? | ? |
| A12 | Tg3p_fusion N term | O18 | O17 | ? (63.5) | ? | 274 | Amplification of N-term domain of M13's g3p | ? | ? | 08/07 |
| A13 | tolRII_fusion N term | O13 | O1 | (73.5) | F4 | 279 | Amplification of tolR | ? | ? | ? |
| A14 | tatABC_RBS | O14 | O15 | (63.5) | F4 | 1634 | Amplification of tatA with RBS | ? | ? | 08/07 |
http://parts.mit.edu/r/parts/images/partbypart/icon_terminator.png
http://parts.mit.edu/images/partbypart/icon_regulatory.png
http://parts.mit.edu/r/parts/images/partbypart/icon_rbs.png
http://parts.mit.edu/r/parts/images/partbypart/icon_reporter.png
http://parts.mit.edu/registry/images/7/7b/Part_icon_cds.png
http://parts.mit.edu/registry/images/9/9f/Part_icon_dna.png
http://parts.mit.edu/registry/images/2/2e/Part_icon_generator.png
http://parts.mit.edu/registry/images/a/ab/Part_icon_plasmid.png
