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Team:TUDelft/7 August 2009
From 2009.igem.org
Lab Notebook
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7 August 2009
Tim Weenink
Did the rest of the assembly transformations. This time TSS and RubidiumCloride chemically competent cells were transformed with all ten constructs. LB medium was used instead of SOC, because all the SOC was used yesterday.
Calin
Designed short knockout primers. CA had colonies. But not all expressed GFP:
| Set | GFP colonies | Non-GFP colonies |
| RbCl | 3 | 140 |
| TSS | 1? | 7 |
| ElectroPoration | 6 | 54 |
The long knockout primers and trbK gene arrived. Made a backup AMP plate for trbK. Rehydrated and electroporated BBa_J13002 (pTet-RBS).
| Set | Time Constant |
| Assembly 1 | 4.4 |
| BBa_J13002 | 4.6 |
Daniel made CAM control plates at both high and low concentrations.
