User contributions
From 2009.igem.org
- 03:52, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling (→The System) (top)
- 03:42, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Vision (→Our Vision) (top)
- 03:40, 22 October 2009 (diff | hist) N File:IGEM Uppsala09.pdf (top)
- 03:39, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Vision (→Our Vision)
- 03:18, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/pir (→Progress) (top)
- 03:17, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Butanol (→Progress) (top)
- 03:16, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Ethanol (→Progress) (top)
- 03:14, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling (→Utilization)
- 03:13, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling (→The System)
- 03:10, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Vision (→Our Vision)
- 03:02, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling
- 02:49, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling (→Modelling of Ethanol Production)
- 02:46, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling (→Modelling of Ethanol Production)
- 02:28, 22 October 2009 (diff | hist) N File:Schematic View uppsala.png (top)
- 02:25, 22 October 2009 (diff | hist) N File:Systemuppsala.png (top)
- 02:24, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling (→Modelling of Ethanol Production)
- 02:24, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling (→Modelling of Ethanol Production)
- 02:22, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling
- 01:15, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling (→Modelling of Ethanol Production)
- 01:13, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling (→Modelling of Ethanol Production)
- 01:06, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling
- 00:59, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling
- 00:52, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling (→Modelling of Ethanol Production)
- 00:36, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/Modelling
- 00:24, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/rna (→Preliminary Results) (top)
- 00:05, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/rna
- 00:03, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/rna (→Status)
- 00:02, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/rna (→Status)
- 00:02, 22 October 2009 (diff | hist) Team:Uppsala-Sweden/rna (→Status)
- 23:59, 21 October 2009 (diff | hist) Team:Uppsala-Sweden/rna
- 23:58, 21 October 2009 (diff | hist) Team:Uppsala-Sweden/rna (→Status)
- 23:58, 21 October 2009 (diff | hist) N File:Progressbar RNA.png (top)
- 23:57, 21 October 2009 (diff | hist) Team:Uppsala-Sweden/rna
- 18:50, 21 October 2009 (diff | hist) Team:Uppsala-Sweden/Team
- 18:49, 21 October 2009 (diff | hist) Team:Uppsala-Sweden/Team
- 18:49, 21 October 2009 (diff | hist) Team:Uppsala-Sweden/Team
- 18:45, 21 October 2009 (diff | hist) File:Upp erik.jpg (uploaded a new version of "Image:Upp erik.jpg") (top)
- 18:43, 21 October 2009 (diff | hist) N File:Upp anders.jpg (top)
- 18:32, 21 October 2009 (diff | hist) Team:Uppsala-Sweden/Team
- 18:31, 21 October 2009 (diff | hist) Team:Uppsala-Sweden/Team
- 18:28, 21 October 2009 (diff | hist) Team:Uppsala-Sweden/Team
- 18:27, 21 October 2009 (diff | hist) N File:Upp erik.jpg
- 18:17, 21 October 2009 (diff | hist) Uppsala-Sweden/17 October 2009 (top)
- 18:14, 21 October 2009 (diff | hist) N Uppsala-Sweden/17 October 2009 (New page: {{Uppsala-Sweden Template}} ==Sending constructs to the Parts registry== ==Plasmid prep== The ePRPRYT and ePRKRYT constructs with pLac were preped today. {{Uppsala-Sweden_Footer}})
- 18:12, 21 October 2009 (diff | hist) Uppsala-Sweden/16 October 2009 (top)
- 18:10, 21 October 2009 (diff | hist) Uppsala-Sweden/15 October 2009 (top)
- 18:07, 21 October 2009 (diff | hist) N Uppsala-Sweden/16 October 2009 (New page: {{Uppsala-Sweden Template}} ==Triparental mating== The {{Uppsala-Sweden_Footer}})
- 18:06, 21 October 2009 (diff | hist) Uppsala-Sweden/15 October 2009
- 18:05, 21 October 2009 (diff | hist) N File:Colony pcr 091015 r2.png (top)
- 18:05, 21 October 2009 (diff | hist) Uppsala-Sweden/15 October 2009
- 18:01, 21 October 2009 (diff | hist) N File:Colony pcr 091015.png (top)
- 18:01, 21 October 2009 (diff | hist) N Uppsala-Sweden/15 October 2009 (New page: {{Uppsala-Sweden Template}} The colonies that were picked yesterday were evaluated on a gel. Image:colony_pcr_091015.png (From top left) 1. Fermentas 1kb ladder 2-6. PRPRYT(AC) 1-5 7...)
- 17:50, 21 October 2009 (diff | hist) N Uppsala-Sweden/14 October 2009 (New page: {{Uppsala-Sweden Template}} ==Evaluating Yesterdays Transformation== Colonies were picked for the constructs PE1B3T, PRPRYT, PRKRYT and PRPRAT. {{Uppsala-Sweden_Footer}}) (top)
- 17:48, 21 October 2009 (diff | hist) N Uppsala-Sweden/13 October 2009 (New page: {{Uppsala-Sweden Template}} ==Evaluating transformation== One colony were picked for PRKRYT on XAK, inoculated and validated through a colony PCR. ==Antisense RNA== The antisense RNA c...) (top)
- 22:40, 20 October 2009 (diff | hist) m Team:Uppsala-Sweden/Butanol (→The Pathway)
- 22:31, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/Ethanol (→The Constructs)
- 22:29, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/Ethanol (→Progress)
- 22:29, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/Ethanol (→The Constructs)
- 22:17, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/pir (→Progress)
- 22:16, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/pir (→Progress)
- 22:11, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/Butanol (→Progress)
- 22:07, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/Ethanol (→Progress)
- 21:48, 20 October 2009 (diff | hist) File:Progressbar pirAB.png (uploaded a new version of "Image:Progressbar pirAB.png") (top)
- 21:44, 20 October 2009 (diff | hist) N File:Progressbar pirAB.png
- 21:43, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/pir
- 21:30, 20 October 2009 (diff | hist) N File:Progressbar butanol.png (top)
- 21:30, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/Butanol
- 21:23, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/Ethanol (→Progress)
- 21:22, 20 October 2009 (diff | hist) N File:Progressbar ethanol.jpg (top)
- 21:22, 20 October 2009 (diff | hist) Team:Uppsala-Sweden/Ethanol (→Progress)
- 20:00, 20 October 2009 (diff | hist) File:Progressbar ethanol.png (uploaded a new version of "Image:Progressbar ethanol.png") (top)
- 19:58, 20 October 2009 (diff | hist) File:Progressbar ethanol.png (uploaded a new version of "Image:Progressbar ethanol.png")
- 19:58, 20 October 2009 (diff | hist) File:Progressbar ethanol.png (uploaded a new version of "Image:Progressbar ethanol.png")
- 21:29, 19 October 2009 (diff | hist) Uppsala-Sweden/19 September 2009 (top)
- 21:28, 19 October 2009 (diff | hist) N File:Experiment 090919.png (top)
- 21:28, 19 October 2009 (diff | hist) Uppsala-Sweden/19 September 2009 (→Transformation protocol evaluation)
- 21:27, 19 October 2009 (diff | hist) Uppsala-Sweden/19 September 2009 (→Transformation protocol evaluation)
- 21:23, 19 October 2009 (diff | hist) Uppsala-Sweden/19 September 2009 (→Transformation protocol evaluation)
- 21:16, 19 October 2009 (diff | hist) N Uppsala-Sweden/19 September 2009 (New page: ==Transformation evaluation== Crappy transformations for some reason, only three colonies for the pUC19 control. Good transformation for the PRYT construct. Re transformation of R-KRYT, ...)
- 20:58, 19 October 2009 (diff | hist) File:Es eva 090917 t.jpg (uploaded a new version of "Image:Es eva 090917 t.jpg") (top)
- 20:56, 19 October 2009 (diff | hist) N File:Es eva 090917 t.jpg
- 20:56, 19 October 2009 (diff | hist) Uppsala-Sweden/17 September 2009 (→Colony PCR of yesterdays transformations) (top)
- 20:34, 19 October 2009 (diff | hist) m Uppsala-Sweden/17 September 2009 (→Colony PCR of yesterdays transformations)
- 20:30, 19 October 2009 (diff | hist) m Uppsala-Sweden/16 September 2009 (→Plasmid prep evaluation) (top)
- 20:21, 19 October 2009 (diff | hist) N File:20090915 digest evaluation2.jpg (top)
- 20:20, 19 October 2009 (diff | hist) Uppsala-Sweden/15 September 2009 (→Picking colonies and colony PCR) (top)
- 19:58, 19 October 2009 (diff | hist) Uppsala-Sweden/15 September 2009 (→Picking colonies and colony PCR)
- 20:07, 18 October 2009 (diff | hist) N File:Progressbar ethanol.png
- 20:06, 18 October 2009 (diff | hist) Team:Uppsala-Sweden/Ethanol
- 17:15, 11 October 2009 (diff | hist) N File:Triparental+Mating.txt (top)
- 17:15, 11 October 2009 (diff | hist) Uppsala-Sweden/11 October 2009 (→Triparentalmating)
- 17:09, 11 October 2009 (diff | hist) N File:091011 Plasmidprep.xls (top)
- 17:09, 11 October 2009 (diff | hist) N Uppsala-Sweden/11 October 2009 (New page: ==Plasmid prep of PRPRAT and PRKRYT== data shown here:Media:091011_Plasmidprep.xls ==Triparentalmating== Today we put our antisense-RNA constructs in to Synecocystis via triparental m...)
- 13:14, 28 September 2009 (diff | hist) m Uppsala-Sweden/28 September 2009
- 13:13, 28 September 2009 (diff | hist) m Uppsala-Sweden/28 September 2009
- 13:12, 28 September 2009 (diff | hist) m Uppsala-Sweden/28 September 2009
- 13:09, 28 September 2009 (diff | hist) N File:Construct+confirm+090929 2.jpg (top)
- 13:09, 28 September 2009 (diff | hist) N Uppsala-Sweden/28 September 2009 (New page: {{Template Uppsala-Sweden}} After having tonnes of problemes with our constructs we were in yhe situation of not trusting any of the constructs. We therefore decided to prform a validatio...)
- 15:13, 21 September 2009 (diff | hist) N File:20090917 colonypc.jpg (top)
- 15:11, 21 September 2009 (diff | hist) Uppsala-Sweden/17 September 2009
- 14:24, 21 September 2009 (diff | hist) N File:20090921 colony PCR R-PpetE-RPRA Pdc-YAdh2 R-KRYT.jpg.jpg (top)
- 14:23, 21 September 2009 (diff | hist) N Uppsala-Sweden/21 September 2009 (New page: {{Uppsala-Sweden Template}} ==Colony PCR of saturdays transformations== Quite a decent transformation all considered, all constructs seem to be working. [[Image:20090921 colony PCR R-Pp...)
- 10:26, 18 September 2009 (diff | hist) N Uppsala-Sweden/17 September 2009 (New page: {{Uppsala-Sweden Template}} ==Colony PCR of yesterdays transformations== The colony PCR was this time performed utilizing dream taq. Weseem to have good transformations of R-Kivd (2), K...)
- 16:43, 8 September 2009 (diff | hist) N File:Assembly volume calcuator 090908.xls (top)
- 16:29, 8 September 2009 (diff | hist) N Uppsala-Sweden/8 September 2009 (New page: {{Uppsala-Sweden Template}} ==Ligation and transformatioin of antisense RNA and other stuff== Performed accordingly: Media:Assembly volume calcuator 090908.xls --~~~~)
- 17:50, 7 September 2009 (diff | hist) N File:Plasmidprep 090907.txt (top)
- 17:49, 7 September 2009 (diff | hist) Uppsala-Sweden/7 September 2009
- 16:12, 31 August 2009 (diff | hist) N File:Ligation voulume calcuator 090831 1.xls (top)
- 16:12, 31 August 2009 (diff | hist) N File:Assembly volume calcuator090831 1.xls (top)
- 16:11, 31 August 2009 (diff | hist) Uppsala-Sweden/31 August 2009 (→Transformation)
- 15:11, 31 August 2009 (diff | hist) N File:Digest purification measur 09831.txt (top)
- 15:11, 31 August 2009 (diff | hist) Uppsala-Sweden/31 August 2009
- 13:20, 31 August 2009 (diff | hist) N File:Digestion(excert)090831.xls (top)
- 13:20, 31 August 2009 (diff | hist) Uppsala-Sweden/31 August 2009
- 13:05, 31 August 2009 (diff | hist) N File:20090831 Adh2Y.jpg (top)
- 13:04, 31 August 2009 (diff | hist) N Uppsala-Sweden/31 August 2009 (New page: {{Uppsala-Sweden Template}} ==Adh2Y PCR== Gel picture, one nice band. Image:20090831_Adh2Y.jpg to be annotated --~~~~)
- 21:42, 30 August 2009 (diff | hist) Team:Uppsala-Sweden/Team
- 14:07, 30 August 2009 (diff | hist) N File:Assembly volume calcuator 090830 2.xls (top)
- 14:07, 30 August 2009 (diff | hist) N File:Assembly volume calcuator 090830 1.xls (top)
- 14:06, 30 August 2009 (diff | hist) N Uppsala-Sweden/30 August 2009 (New page: {{Uppsala-Sweden Template}} ==Assembly== Today we well assemble the constructs RBS-pdc_RBS-adh2Z, RBS-pirB-Terminator, PpetE-RBS. Volumes in these files [[Media:Assembly volume calcuator...)
- 16:11, 29 August 2009 (diff | hist) N File:Digestion 090829.txt (top)
- 16:11, 29 August 2009 (diff | hist) Uppsala-Sweden/29 August 2009 (top)
- 16:10, 29 August 2009 (diff | hist) Uppsala-Sweden/29 August 2009
- 13:06, 29 August 2009 (diff | hist) N File:Plasmidprep 090829 2.txt (top)
- 13:05, 29 August 2009 (diff | hist) N File:Plasmidprep 090829.txt (top)
- 13:05, 29 August 2009 (diff | hist) N Uppsala-Sweden/29 August 2009 (New page: {{Uppsala-Sweden Template}} ==Plasmid prep of RBS + PDC construct== Measurement of the plasmid prep from the RBS + PDC construct, 50 times dilution. Media:Plasmidprep_090829.txt ==Te...)
- 10:41, 28 August 2009 (diff | hist) Uppsala-Sweden/27 August 2009 (top)
- 17:29, 27 August 2009 (diff | hist) Uppsala-Sweden/27 August 2009
- 17:28, 27 August 2009 (diff | hist) N Uppsala-Sweden/27 August 2009 (New page: {{Uppsala-Sweden Template}} ==Assembly evaluation== Here are he images of the evaluation gel f yesterdays assembly: Upper Image:20090827 colonypcr_1 Lower [[Image:20090827 colony...)
- 17:28, 27 August 2009 (diff | hist) N File:20090827 colonypcr 2.jpg (top)
- 17:27, 27 August 2009 (diff | hist) N File:20090827 colonypcr 1.jpg (top)
- 20:33, 26 August 2009 (diff | hist) Uppsala-Sweden/26 August 2009 (top)
- 10:33, 25 August 2009 (diff | hist) N Uppsala-Sweden/24 August 2009 (New page: {{Uppsala-Sweden Template}} ==Preparaing competent cells== Today 160 2 ml tubes with 50 µl top ten cells each were prepared and put in the - 80 freezer according to the [http://openwetwa...) (top)
- 19:34, 17 August 2009 (diff | hist) N Uppsala-Sweden/17 August 2009 (New page: {{Uppsala-Sweden Template}} ==Top10 competent cells, making== As our earlier competent cells weren't working very well we decided to make new ones and in the process switch to the seeming...)
- 15:45, 13 August 2009 (diff | hist) m Uppsala-Sweden/13 August 2009
- 15:44, 13 August 2009 (diff | hist) N Uppsala-Sweden/13 August 2009 (New page: {{Template Uppsala-Sweden}} ==Transformation of ADH2 from z.mobilis, continued== Unfortunatly we only got two (!!) colonies from Adh2 z. These are most likely to be contamination as one w...)
- 15:28, 13 August 2009 (diff | hist) m Uppsala-Sweden/12 August 2009
- 15:35, 11 August 2009 (diff | hist) N Uppsala-Sweden/11 August 2009 (New page: {{Uppsala-Sweden Template}} ==Evaluation of competent cells== Due to the run of bad transformations we've experienced we decided to evaluate the quality of our cells. We used our previosl...)
- 15:20, 11 August 2009 (diff | hist) m Uppsala-Sweden/10 August 2009
- 20:00, 1 August 2009 (diff | hist) Uppsala-Sweden/31 July 2009 (top)
- 19:47, 1 August 2009 (diff | hist) Uppsala-Sweden/30 July 2009 (top)
- 19:45, 1 August 2009 (diff | hist) Uppsala-Sweden/31 July 2009
- 19:38, 1 August 2009 (diff | hist) Uppsala-Sweden/30 July 2009
- 13:57, 30 July 2009 (diff | hist) N Uppsala-Sweden/29 July 2009 (New page: {{Uppsala-Sweden Template}} '''Competent cell preperation''' We made CCMB80 buffer and inoculated four 2 ml cultures of competent E.coli as given by: [http://openwetware.org/wiki/TOP10_...) (top)
- 13:49, 30 July 2009 (diff | hist) N Uppsala-Sweden/30 July 2009 (New page: {{Uppsala-Sweden Template}} '''kivd adhII pdc PCR''' Today we begun the trials to extract the kivd gene from Lactococcus lactis, and adhII and pdc from S.cerivase. We will try to extrac...)
- 11:53, 23 July 2009 (diff | hist) N Uppsala-Sweden/23 July 2009 (New page: {{Uppsala-Sweden Template}} '''Butanol survival''' OD Mesurment 11:48 Marked growt in 0% Degeneration of OD in higher concentrations --~~~~) (top)
- 11:51, 23 July 2009 (diff | hist) Uppsala-Sweden/22 July 2009 (top)
- 11:50, 23 July 2009 (diff | hist) N Uppsala-Sweden/22 July 2009 (New page: {{Uppsala-Sweden Template}} '''Butanol Survival''' OD Measurment 13:31 Growth of 0% others at stand still --~~~~)
- 11:47, 23 July 2009 (diff | hist) Uppsala-Sweden/21 July 2009
- 11:43, 23 July 2009 (diff | hist) N File:Butanol+survival+test+conc090721.xls (Experiment set up butanol suvival 090721) (top)
- 11:40, 23 July 2009 (diff | hist) Uppsala-Sweden/21 July 2009
- 11:29, 23 July 2009 (diff | hist) Uppsala-Sweden/20 July 2009 (top)
- 12:03, 19 July 2009 (diff | hist) Uppsala-Sweden/15 July 2009 (top)
- 12:02, 19 July 2009 (diff | hist) N Uppsala-Sweden/15 July 2009 (New page: '''Determination of linear range OD culture measurement.''' Start culture was diluted 1 2 4 8 16 32 64 128 parts to final volume 200 µl on 96-well plate with BG-11 growth media, triplica...)
- 11:55, 19 July 2009 (diff | hist) Uppsala-Sweden/17 July 2009
- 11:21, 19 July 2009 (diff | hist) Uppsala-Sweden/16 July 2009
- 11:20, 19 July 2009 (diff | hist) N Uppsala-Sweden/16 July 2009 (Start of ethanol survival experiment.)