Team:Southampton/Project/Protocols

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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Intro">Project Introduction</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Intro">Project Introduction</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Achievements">Project Achievements</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Projection">Project Projection</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Projection">Project Projection</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Safety">Project Safety</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Refer">References and Links</a></li>
         <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
         <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1">Protocol Theory</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/ColonyPCR">Colony PCR</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/ColonyPCR">Colony PCR</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts">Biobrick Parts</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="#">Biobrick Parts<![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team"><span>Team</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team"><span>Team</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Southampton">Southampton Profile</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/University">University Profile</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling">Modelling Overview</a></li>
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         <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/GoL">Game Of Life</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/RPS">Rock Paper Scissors</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/Open">Open Access</a></li>
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         <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
         <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/igemexper">iGEM Experience</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/igemexper">iGEM Experience</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Political">Political Awareness</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Political">Political Awareness</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Legacy">Our Legacy</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors">Sponsors</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors">Sponsors</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Southampton">Southampton</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Southampton">Team Southampton</a></li>
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   <h3>As part of the preparation for iGEM 2009, we have peformed a number of experimental processes which are detailed in protocols. We are making these protocols available on our wiki to help future teams in their synthetic approach. These protocols can be accesed together with an explanation of the theoretical basis using the links embedded in the diagram below:</h3>
   <h3>As part of the preparation for iGEM 2009, we have peformed a number of experimental processes which are detailed in protocols. We are making these protocols available on our wiki to help future teams in their synthetic approach. These protocols can be accesed together with an explanation of the theoretical basis using the links embedded in the diagram below:</h3>
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         <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/trans.gif"  width="338" height="84" rel="lightbox[pro]" title="Transformation - Single plasmids are introduced into recipient bacterial cells. The cells are allowed to replicate in order to achieve high yields of plasmid DNA."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_03.gif" width="338" height="84" border="0" /></a></td>
        
        
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         <img id="Untitled_1_14" src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_14.gif" width="75" height="498" alt="" /></td>
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         <img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_14.gif" alt="" name="Untitled_1_14" width="75" height="498" border="0" id="Untitled_1_14" /></td>
        
        
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         <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/gel.gif"  width="186" height="174" rel="lightbox" title="Gel Electrophoresis - Distance travelled by the DNA helps identify the DNA molecules present."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_15.gif" width="186" height="174" border="0" /></td>
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         <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/gel.gif"  width="186" height="174" rel="lightbox[pro]" title="Gel Electrophoresis - Distance travelled by the DNA helps identify the DNA molecules present."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_15.gif" width="186" height="174" border="0" /></td>
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       <td colspan="4" rowspan="3" bgcolor="#5D5D5D">
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         <img id="Untitled_1_16" src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_16.gif" width="178" height="85" alt="" /></td>
+
         <img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_16.gif" alt="" name="Untitled_1_16" width="178" height="85" border="0" id="Untitled_1_16" /></td>
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       <td bgcolor="#5D5D5D">
         <img src="http://www.personal.soton.ac.uk/jeh2v07/images/spacer.gif" width="1" height="14" alt="" /></td>
         <img src="http://www.personal.soton.ac.uk/jeh2v07/images/spacer.gif" width="1" height="14" alt="" /></td>
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       </tr>
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       <td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#pcr"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_17.gif" alt="" name="Untitled_1_17" width="171" height="35" border="0" id="Untitled_1_17" /></a></td>
+
       <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#pcr"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_17.gif" alt="" name="Untitled_1_17" width="171" height="35" border="0" id="Untitled_1_17" /></a></td>
-
       <td><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Hfpcr"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_18.gif" alt="" name="Untitled_1_18" width="167" height="35" border="0" id="Untitled_1_18" /></a></td>
+
       <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Hfpcr"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_18.gif" alt="" name="Untitled_1_18" width="167" height="35" border="0" id="Untitled_1_18" /></a></td>
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         <img id="Untitled_1_19" src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_19.gif" width="338" height="60" alt="" /></td>
         <img id="Untitled_1_19" src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_19.gif" width="338" height="60" alt="" /></td>
        
        
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       <td rowspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_20.gif" alt="" name="Untitled_1_20" width="143" height="38" border="0" id="Untitled_1_20" /></a></td>
+
       <td width="143" rowspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_20.gif" alt="" name="Untitled_1_20" width="143" height="38" border="0" id="Untitled_1_20" /></a></td>
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         <img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_21.gif" alt="" name="Untitled_1_21" width="35" height="413" border="0" id="Untitled_1_21" /></td>
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         <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/digest.gif"  width="338" height="84" rel="lightbox" title="Digestion - DNA strands are cut to either remove a sequence of base pairs or open up a plasmid."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_22.gif" width="338" height="150" border="0" /></td>
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         <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/digest.gif"  width="338" height="84" rel="lightbox[pro]" title="Digestion - DNA strands are cut to either remove a sequence of base pairs or open up a plasmid."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_22.gif" width="338" height="150" border="0" /></td>
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       <td><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_23.gif" alt="" name="Untitled_1_23" width="143" height="51" border="0" id="Untitled_1_23" /></a></td>
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       <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Gel"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_23.gif" alt="" name="Untitled_1_23" width="143" height="51" border="0" id="Untitled_1_23" /></a></td>
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       <td bgcolor="#5D5D5D">
         <img src="http://www.personal.soton.ac.uk/jeh2v07/images/spacer.gif" width="1" height="51" alt="" /></td>
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         <img id="Untitled_1_24" src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_24.gif" width="329" height="324" alt="" /></td>
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         <img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_24.gif" alt="" name="Untitled_1_24" width="329" height="324" border="0" id="Untitled_1_24" /></td>
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       <td><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#digestion"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_25.gif" alt="" name="Untitled_1_25" width="162" height="40" border="0" id="Untitled_1_25" /></a></td>
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       <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#digestion"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_25.gif" alt="" name="Untitled_1_25" width="162" height="40" border="0" id="Untitled_1_25" /></a></td>
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       <td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/DoubleDigestion"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_26.gif" alt="" name="Untitled_1_26" width="176" height="40" border="0" id="Untitled_1_26" /></a></td>
+
       <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/DoubleDigestion"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_26.gif" alt="" name="Untitled_1_26" width="176" height="40" border="0" id="Untitled_1_26" /></a></td>
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         <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/ligate.gif"  width="338" height="88" rel="lightbox" title="Ligation - Two samples of DNA are joined together."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_28.gif" width="338" height="88" border="0" /></td>
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         <a href="http://www.personal.soton.ac.uk/jeh2v07/images/ProtocolL/ligate.gif"  width="338" height="88" rel="lightbox[pro]" title="Ligation - Two samples of DNA are joined together."><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_28.gif" width="338" height="88" border="0" /></td>
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       <td colspan="2"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#ligation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_29.gif" alt="" name="Untitled_1_29" width="171" height="36" border="0" id="Untitled_1_29" /></a></td>
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       <td colspan="2" bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1#ligation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_29.gif" alt="" name="Untitled_1_29" width="171" height="36" border="0" id="Untitled_1_29" /></a></td>
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       <td><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_30.gif" alt="" name="Untitled_1_30" width="167" height="36" border="0" id="Untitled_1_30" /></a></td>
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       <td bgcolor="#5D5D5D"><a href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligation"><img src="http://www.personal.soton.ac.uk/jeh2v07/images/Untitled-1_30.gif" alt="" name="Untitled_1_30" width="167" height="36" border="0" id="Untitled_1_30" /></a></td>
        
        
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Southampton iGEM 2009
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Southampton iGEM 2009</h5>
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Latest revision as of 19:36, 20 October 2009

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> University of Southampton Wiki

Protocol Map

 

 

As part of the preparation for iGEM 2009, we have peformed a number of experimental processes which are detailed in protocols. We are making these protocols available on our wiki to help future teams in their synthetic approach. These protocols can be accesed together with an explanation of the theoretical basis using the links embedded in the diagram below:

   
                   
| Top |          University of Southampton iGEM 2009

Retrieved from "http://2009.igem.org/Team:Southampton/Project/Protocols"