Team:Southampton/Parts/GoL

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Revision as of 19:28, 21 October 2009

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> University of Southampton Wiki

Biobrick Parts: Rock Paper Scissors

 

 

 

 

For a list of all our parts, please click here.


To view the parts used in our Game of Life sub-project, click here.

 

Part Name

Type

Description

Team Favourite?

Length

K180000

Regulatory

Hybrid promoter (trp & lac regulated – tac pR)
A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.

Yes

1447

K180002

Composite

tac pR testing plasmid (GFP)
Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.

Yes

2330

K180010

Composite

Rock-paper-scissors – Rock primary plasmid
Primary plasmid for rock part of rock-paper-scissors game. Must be used in conjunction with part K180013.

Yes

3888

K180013

Composite

Rock-paper-scissors – Rock secondary plasmid
[rhl pR, LacI generator]
Secondary plasmid for rock part of rock-paper-scissors game. Must be used in conjunction with part K180010.

Yes

1369

K180014

Composite

Rock-paper-scissors – Paper primary plasmid
Primary plasmid for paper part of rock-paper-scissors game. Must be used in conjunction with K180016.

Yes

3952

K180016

Composite

Rock-paper-scissors – Paper secondary plasmid
[lux pR, LacI generator]
Secondary plasmid for paper part of rock-paper-scissors game. Must be used in conjunction with K180014.

Yes

1371

K180017

Composite

Rock-paper-scissors – Scissors primary plasmid
Primary plasmid for scissors part of rock-paper-scissors game. Must be used in conjunction with K180020.

Yes

3858

K180020

Composite

Rock-paper-scissors – Scissors secondary plasmid
[las pR, LacI generator]
Primary plasmid for scissors part of rock-paper-scissors game. Must be used in conjunction with K180017.

Yes

1473

 

 

 

 

 

K180003

Composite

tac pR testing plasmid (GFP) – basic
Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.

 

2173

K180005

Composite

Paper – Primary plasmid (part 1)
[LuxR generator]
Part of the paper primary plasmid; constitutively produces LuxR.

 

979

K180008

Coding

mCherry (rights owned by Clontech)
mCherry fluorescent protein – a derivative of the DsRed fluorescent protein with a short maturation time but lower relative brightness.

 

708

K180009

Coding

mBanana (rights owned by Clontech)
mBanana fluorescent protein – a derivative of the DsRed fluorescent protein with a longer maturation time and lower relative brightness than mCherry.

 

708

K180011

Composite

Rock – Primary plasmid (part 1)
[RhlR generator]
Part of the rock primary plasmid; constitutively produces RhlR.

 

927

K180012

Composite

Rock – Primary plasmid (part 2)
[tac pR, mCherry and LasI generator]
Part of the rock primary plasmid; generates mCherry and LasI under tac pR regulation.

 

2953

K180015

Composite

Paper – Primary plasmid (part 2)
[tac pR, GFP and RhlI generator]
Part of the paper primary plasmid; generates GFP and RhlI under tac pR regulation.

 

2965

K180018

Composite

Scissors – Primary plasmid (part 1)
[LasR generator]
Part of the scissors primary plasmid; constitutively produces LasR.

 

921

K180019

Composite

Scissors – Primary plasmid (part 2)
[tac pR, mBanana and LuxI generator]
Part of the scissors primary plasmid; generates mBanana and LuxI under tac pR regulation.

 

2929

 
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