Team:Southampton/Notebook
From 2009.igem.org
(Difference between revisions)
m |
m |
||
(55 intermediate revisions not shown) | |||
Line 6: | Line 6: | ||
<title>University of Southampton | <title>University of Southampton | ||
Wiki</title> | Wiki</title> | ||
+ | <link rel="stylesheet" href="http://www.personal.soton.ac.uk/jeh2v07/css/menu.css" type="text/css"/> | ||
+ | <link rel="stylesheet" href="http://www.personal.soton.ac.uk/jeh2v07/css/style.css" type="text/css"/> | ||
<style type= "text/css"> | <style type= "text/css"> | ||
+ | /*TOOLBAR*/ | ||
+ | #toolbar { | ||
+ | position:absolute; | ||
+ | left:-2px; | ||
+ | top:0px; | ||
+ | width:978px; | ||
+ | height:27px; | ||
+ | z-index:100; | ||
+ | } | ||
/*FOOTERPUSH*/ | /*FOOTERPUSH*/ | ||
#footerpush { | #footerpush { | ||
width:1px; | width:1px; | ||
- | height: | + | height:2073px; |
} | } | ||
html, body { | html, body { | ||
Line 18: | Line 29: | ||
/*LAYOUT BEGINS*/ | /*LAYOUT BEGINS*/ | ||
#notebooktable { | #notebooktable { | ||
- | + | width: 95%; | |
- | + | ||
- | + | ||
- | width: | + | |
} | } | ||
- | + | #maincont{ | |
- | + | ||
- | # | + | |
position:absolute; | position:absolute; | ||
left:0px; | left:0px; | ||
- | top: | + | top:296px; |
width:975px; | width:975px; | ||
- | height: | + | height:1850px; |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
background-image:url('http://www.personal.soton.ac.uk/jeh2v07/images/bg.gif'); | background-image:url('http://www.personal.soton.ac.uk/jeh2v07/images/bg.gif'); | ||
background-repeat:repeat-y; | background-repeat:repeat-y; | ||
Line 127: | Line 42: | ||
padding: 0; | padding: 0; | ||
} | } | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
#sufooter { | #sufooter { | ||
position:absolute; | position:absolute; | ||
left:113px; | left:113px; | ||
- | top: | + | top:2115px; |
z-index:150; | z-index:150; | ||
color: #333; | color: #333; | ||
Line 409: | Line 52: | ||
position:absolute; | position:absolute; | ||
left:0px; | left:0px; | ||
- | top: | + | top:2085px; |
width:975px; | width:975px; | ||
height:61px; | height:61px; | ||
z-index:100; | z-index:100; | ||
+ | } | ||
</style> | </style> | ||
</head> | </head> | ||
<body style="color: rgb(0, 0, 0); background-color: #919191;" | <body style="color: rgb(0, 0, 0); background-color: #919191;" | ||
- | link="#000099" alink="#000099" vlink="#990099"> | + | link="#000099" alink="#000099" vlink="#990099"><div id="footerpush"></div> |
<div id="banner"><img style="width: 975px; height: 276px;" id="banner" | <div id="banner"><img style="width: 975px; height: 276px;" id="banner" | ||
- | src="http://www.personal.soton.ac.uk/jeh2v07/images/ | + | src="http://www.personal.soton.ac.uk/jeh2v07/images/grey.jpg" alt="" /></div> |
<div id="toolbar"> | <div id="toolbar"> | ||
<ul class="cssMenu cssMenum"> | <ul class="cssMenu cssMenum"> | ||
- | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton">Home</a></li> | |
- | href="https://2009.igem.org/Team:Southampton"> | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> |
- | + | <ul class=" cssMenum"> | |
- | href="https://2009.igem.org/Team:Southampton/Project">Project</a></li> | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Aims">Project Aims</a></li> |
- | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Intro">Project Introduction</a></li> | |
- | href="https://2009.igem.org/Team:Southampton/ | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li> |
- | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Achievements">Project Achievements</a></li> | |
- | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Projection">Project Projection</a></li> | |
- | href="https://2009.igem.org/Team:Southampton/Team">< | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Safety">Project Safety</a></li> |
- | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Refer">References and Links</a></li> | |
- | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> | |
- | href="https://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li> | + | <ul class=" cssMenum"> |
- | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li> | |
- | <!--[if lte IE 6]></td></tr></table></a><![endif]--></li> | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1">Protocol Theory</a></li> |
- | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li> | |
- | href="https://2009.igem.org/Team:Southampton/Modeling">Modelling</a></li> | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/ColonyPCR">Colony PCR</a></li> |
- | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/DoubleDigestion">DNA Double Digestion</a></li> | |
- | href="https://2009.igem.org/Team:Southampton/Notebook">Notebook</a></li> | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Gel">Gel Electrophoresis</a></li> |
- | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Hfpcr">High Fidelity PCR</a></li> | |
- | href="https://2009.igem.org/Team:Southampton/Sponsors">Sponsors</a></li> | + | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/LBplates">LB Plates</a></li> |
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligation">Ligation</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li> | ||
+ | </ul> | ||
+ | <!--[if lte IE 6]></td></tr></table></a><![endif]--></li> | ||
+ | </ul> | ||
+ | <!--[if lte IE 6]></td></tr></table></a><![endif]--></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="#">Biobrick Parts<![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> | ||
+ | <ul class=" cssMenum"> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li> | ||
+ | </ul></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Team"><span>Team</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> | ||
+ | <ul class=" cssMenum"> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Southampton">Southampton Profile</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Team/University">University Profile</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li> | ||
+ | </ul> | ||
+ | <!--[if lte IE 6]></td></tr></table></a><![endif]--></li> | ||
+ | |||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling"><span>Modelling</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> | ||
+ | <ul class=" cssMenum"> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling">Modelling Overview</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/GoL">Game Of Life</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/RPS">Rock Paper Scissors</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/Open">Open Access</a></li> | ||
+ | </ul> | ||
+ | <!--[if lte IE 6]></td></tr></table></a><![endif]--></li> | ||
+ | |||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/HP"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> | ||
+ | <ul class=" cssMenum"> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/HP">Overview</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Outreach">Outreach</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/HP/igemexper">iGEM Experience</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Political">Political Awareness</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Legacy">Our Legacy</a></li> | ||
+ | </ul> | ||
+ | <!--[if lte IE 6]></td></tr></table></a><![endif]--></li> | ||
+ | |||
+ | |||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook">Notebook</a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> | ||
+ | <ul class=" cssMenum"> | ||
+ | |||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook">Notebook</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook/Labbook">Lab Book</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook/Advisorblog">Advisor Blog</a></li> | ||
+ | </ul> | ||
+ | <!--[if lte IE 6]></td></tr></table></a><![endif]--></li> | ||
+ | |||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors"><span>Acknowledgments</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> | ||
+ | <ul class=" cssMenum"> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors">Sponsors</a></li> | ||
+ | <li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Southampton">Team Southampton</a></li> | ||
+ | </ul> | ||
+ | <!--[if lte IE 6]></td></tr></table></a><![endif]--></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
Line 448: | Line 150: | ||
<img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /></div> | <img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /></div> | ||
<div id="projectheading"> | <div id="projectheading"> | ||
- | < | + | <h1>Notebook</h1> |
</div> | </div> | ||
<div id="maincont"> | <div id="maincont"> | ||
Line 461: | Line 163: | ||
<tbody> | <tbody> | ||
<tr> | <tr> | ||
- | <td width=" | + | <td width="30%"><h4>Week |
Commencing</h4></td> | Commencing</h4></td> | ||
- | <td width=" | + | <td width="70%"><h4>Summary Of The Week</h4></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Line 547: | Line 249: | ||
Digest…ligate…transform<br /> | Digest…ligate…transform<br /> | ||
Digest…ligate…transform<br /> | Digest…ligate…transform<br /> | ||
- | Shame none of them worked… | + | Shame none of them worked… |
- | On the plus side, our simulations are | + | On the plus side, our simulations are |
looking good!</h3></td> | looking good!</h3></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><h3>31st August 2009</h3></td> | <td><h3>31st August 2009</h3></td> | ||
- | <td><h3>The team begins to feel pretty | + | <td><h3>The team begins to feel pretty |
- | demoralised as the second week of | + | demoralised as the second week of |
- | ‘digest…ligate…transform…FAIL’ | + | ‘digest…ligate…transform…FAIL’ |
- | commences. | + | commences. |
- | We decide to momentarily focus on building our ‘novel’ biobrick. | + | We decide to momentarily focus on building our ‘novel’ biobrick. |
- | Good news though, our simulations | + | Good news though, our simulations |
- | specific to RPS/GoL are complete. Now | + | specific to RPS/GoL are complete. Now |
- | to make them applicable to | + | to make them applicable to |
- | anything/everything else! | + | anything/everything else! |
- | Work starts on our synthetic biology | + | Work starts on our synthetic biology |
- | video…soon to be the most demanded | + | video…soon to be the most demanded |
- | on YouTube. | + | on YouTube. |
- | Our wiki page has been transformed. | + | Our wiki page has been transformed. |
- | Just you wait for the launch next week. | + | Just you wait for the launch next week. |
- | Mesmerising. | + | Mesmerising. |
- | We are warned about round two of | + | We are warned about round two of |
- | abandonment by team supervisor. | + | abandonment by team supervisor. |
- | ‘Climbing’ is the new excuse. Not even | + | ‘Climbing’ is the new excuse. Not even |
blaming the family this time!</h3></td> | blaming the family this time!</h3></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><h3>7th September 2009</h3></td> | <td><h3>7th September 2009</h3></td> | ||
- | <td><h3>We think we have finally optimised our | + | <td><h3>We think we have finally optimised our |
- | digest…ligate technique. However, | + | digest…ligate technique. However, |
- | when we come to transform, we have a | + | when we come to transform, we have a |
- | distinct lack of competent cells. Whose | + | distinct lack of competent cells. Whose |
- | authorisation do we need? The project | + | authorisation do we need? The project |
- | supervisor. And where is he? Turkey. | + | supervisor. And where is he? Turkey. |
- | Splendid. | + | Splendid. |
- | Instead, we concentrate on more | + | Instead, we concentrate on more |
- | ‘behind the scenes’ such as content for | + | ‘behind the scenes’ such as content for |
- | our wiki. It’s going well, and we are | + | our wiki. It’s going well, and we are |
- | collecting information fast. | + | collecting information fast. |
- | Progress is also being made with | + | Progress is also being made with |
- | regard to the video, the script is almost | + | regard to the video, the script is almost |
- | complete, and filming is due to start | + | complete, and filming is due to start |
- | early next week. Now, who wants to be | + | early next week. Now, who wants to be |
- | in it? | + | in it? |
- | To fill the lack of time spent in the labs, | + | To fill the lack of time spent in the labs, |
- | we decided to do a ‘fun’ side project, to | + | we decided to do a ‘fun’ side project, to |
- | optimize SYBR gold gels. It failed. Yes, | + | optimize SYBR gold gels. It failed. Yes, |
- | an experiment with no use other than | + | an experiment with no use other than |
to fill time, failed. Time to go home…</h3></td> | to fill time, failed. Time to go home…</h3></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><h3>14th September 2009</h3></td> | ||
+ | <td><h3>The competent cells finally arrive and we are all frantically busy in labs again. Has anything been successful yet? No. | ||
+ | One of our post grad supervisors makes some dodgy minimal media. As in, it was cloudy and brown. ‘It’s okay he said, they will set clear…’ | ||
+ | Meanwhile, our other supervisor threw our ligation products out, only to root around the bin and find that they had kept the right ones in the first place… we look up to our supervisors, we really do. Our first iGEM romance becomes apparent. We’re just surprised it’s taken this long to occur! | ||
+ | Our project supervisor returns sporting the most fantastic suit. Just imagine, beige chinos, brown shoes, white shirt, gold tie and black, velvet jacket. Stunning. | ||
+ | |||
+ | </h3></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td><h3>21st September 2009</h3></td> | ||
+ | <td><h3>So, onto the final week, and what do we have to show for our iGEM experience? | ||
+ | We performed TA cloning on our novel biobricks; pTac and GFP. This is good news, as it is the first (and only) successful ligation so far… | ||
+ | We have a final meeting and farewell with a few refreshments…it starts off well as one of our supervisors walks in and immediately says ‘so, we’re celebrating failure now are we?!’ That didn’t hurt at all. Much. | ||
+ | The wiki is nearly completed, the outreach projects have been successful, and the simulations are ready to be uploaded for the world to see. It’s a shame about the lab work, it really is. | ||
+ | Last ever cluster map update: 26 different countries totalling nearly 400 visits…we just won’t mention how many of those were from the UK (ahem….Southampton). | ||
+ | Finally, our project supervisor drops one last bombshell…he’s not joining us in America. | ||
+ | Guess the excuse this time… | ||
+ | </h3></td> | ||
</tr> | </tr> | ||
</tbody> | </tbody> | ||
Line 601: | Line 323: | ||
</div> | </div> | ||
<div id="sufooter"> | <div id="sufooter"> | ||
- | + | <h5>| <a href="#">Top</a> | | |
- | + | ||
- | + | ||
| | ||
University of | University of | ||
- | Southampton iGEM 2009 | + | Southampton iGEM 2009</h5> |
</div> | </div> | ||
<div id="contfoot"> | <div id="contfoot"> | ||
<img src="http://www.personal.soton.ac.uk/jeh2v07/images/mainfoot.gif" width="975" height="61" /></div></body> | <img src="http://www.personal.soton.ac.uk/jeh2v07/images/mainfoot.gif" width="975" height="61" /></div></body> | ||
</html> | </html> |
Latest revision as of 19:58, 21 October 2009
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
Notebook
Week Commencing |
Summary Of The Week |
20th July 2009 |
‘Brainstorming’
becomes our key word. |
27th July 2009 |
Work begins on our wiki
page and logo design. |
3rd August 2009 |
The hard works
begins… |
10th August 2009 |
Man back :) , he just
could not stay away. |
17th August 2009 |
We are granted
permission to start an outreach project …hold tight folks,
more details coming soon. |
25th August 2009 |
We perfect a pattern: |
31st August 2009 |
The team begins to feel pretty demoralised as the second week of ‘digest…ligate…transform…FAIL’ commences. We decide to momentarily focus on building our ‘novel’ biobrick. Good news though, our simulations specific to RPS/GoL are complete. Now to make them applicable to anything/everything else! Work starts on our synthetic biology video…soon to be the most demanded on YouTube. Our wiki page has been transformed. Just you wait for the launch next week. Mesmerising. We are warned about round two of abandonment by team supervisor. ‘Climbing’ is the new excuse. Not even blaming the family this time! |
7th September 2009 |
We think we have finally optimised our digest…ligate technique. However, when we come to transform, we have a distinct lack of competent cells. Whose authorisation do we need? The project supervisor. And where is he? Turkey. Splendid. Instead, we concentrate on more ‘behind the scenes’ such as content for our wiki. It’s going well, and we are collecting information fast. Progress is also being made with regard to the video, the script is almost complete, and filming is due to start early next week. Now, who wants to be in it? To fill the lack of time spent in the labs, we decided to do a ‘fun’ side project, to optimize SYBR gold gels. It failed. Yes, an experiment with no use other than to fill time, failed. Time to go home… |
14th September 2009 |
The competent cells finally arrive and we are all frantically busy in labs again. Has anything been successful yet? No. One of our post grad supervisors makes some dodgy minimal media. As in, it was cloudy and brown. ‘It’s okay he said, they will set clear…’ Meanwhile, our other supervisor threw our ligation products out, only to root around the bin and find that they had kept the right ones in the first place… we look up to our supervisors, we really do. Our first iGEM romance becomes apparent. We’re just surprised it’s taken this long to occur! Our project supervisor returns sporting the most fantastic suit. Just imagine, beige chinos, brown shoes, white shirt, gold tie and black, velvet jacket. Stunning. |
21st September 2009 |
So, onto the final week, and what do we have to show for our iGEM experience? We performed TA cloning on our novel biobricks; pTac and GFP. This is good news, as it is the first (and only) successful ligation so far… We have a final meeting and farewell with a few refreshments…it starts off well as one of our supervisors walks in and immediately says ‘so, we’re celebrating failure now are we?!’ That didn’t hurt at all. Much. The wiki is nearly completed, the outreach projects have been successful, and the simulations are ready to be uploaded for the world to see. It’s a shame about the lab work, it really is. Last ever cluster map update: 26 different countries totalling nearly 400 visits…we just won’t mention how many of those were from the UK (ahem….Southampton). Finally, our project supervisor drops one last bombshell…he’s not joining us in America. Guess the excuse this time… |