Team:Southampton

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   <title>University of Southampton
   <title>University of Southampton
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iGEM 2009 Wiki</title>
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Wiki</title>
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<ul class="cssMenu cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton">Home</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Aims">Project Aims</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Intro">Project Introduction</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Achievements">Project Achievements</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Projection">Project Projection</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Safety">Project Safety</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Refer">References and Links</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1">Protocol Theory</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/ColonyPCR">Colony PCR</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/DoubleDigestion">DNA Double Digestion</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Gel">Gel Electrophoresis</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Hfpcr">High Fidelity PCR</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/LBplates">LB Plates</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligation">Ligation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Biobrick Parts<![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li>
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</ul></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team"><span>Team</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Southampton">Southampton Profile</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/University">University Profile</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li>
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</ul>
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            <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling"><span>Modelling</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling">Modelling Overview</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/GoL">Game Of Life</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/RPS">Rock Paper Scissors</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/Open">Open Access</a></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
 +
             
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP">Overview</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Outreach">Outreach</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/igemexper">iGEM Experience</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Political">Political Awareness</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Legacy">Our Legacy</a></li>
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</ul>
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 +
   
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook">Notebook</a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<!-- Save for Web Slices (index(master).psd) -->
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook">Notebook</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook/Labbook">Lab Book</a></li>
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<div id="index-master--01"><img
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook/Advisorblog">Advisor Blog</a></li>
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    <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors"><span>Acknowledgments</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Southampton">Team Southampton</a></li>
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<div id="bg"><img style="width: 975px; height: 1005px;" id="bg"
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<img
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  src="http://www.personal.soton.ac.uk/jeh2v07/images/BGHOME.gif" alt="" /></div>
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<div id="logobox">
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<img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /></div>
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<div id="projectheading">
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<h1>Project Description</h1>
 +
 
</div>
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<div id="bluesubtitle">
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<h2>01/08/2009 - Project</h2>
</div>
</div>
-
<div id="wrap">
+
<div id="projdesc">
-
<div class="pagewrapper">
+
<h3>As a new team to the iGEM
-
<div class="innerpagewrapper">
+
competition, Southampton hopes to bring
-
<div class="page">
+
an innovative application of quorum sensing to the 2009 Jamboree. Using
-
<div class="content"><!-- CONTENT -->
+
-
<h3> </h3>
+
-
<table style="text-align: left; width: 100%;"
+
-
border="0" cellpadding="2" cellspacing="2">
+
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  <tbody>
+
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    <tr>
+
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      <td>
+
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      <h3><img
+
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style="width: 100px; height: 100px;" alt="iGEM"
+
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src="http://img34.imageshack.us/img34/4945/igembox1.jpg" /></h3>
+
-
      </td>
+
-
      <td>
+
-
      <h3>&gt;&gt; Welcome</h3>
+
-
      <p>Welcome to the University
+
-
of
+
-
Southampton 2009 Wiki page</p>
+
-
      </td>
+
-
    </tr>
+
-
  </tbody>
+
-
</table>
+
-
<h3>Project Description</h3>
+
-
<p>As a new team to the iGEM
+
-
competition, Southampton hopes to bring an
+
-
innovative application of quorum sensing to the 2009 Jamboree. Using
+
this method of bacterial communication, we have designed two systems,
this method of bacterial communication, we have designed two systems,
which demonstrate the classic games
which demonstrate the classic games
&lsquo;Rock-Paper-Scissors&rsquo;
&lsquo;Rock-Paper-Scissors&rsquo;
-
and &lsquo;Conway&rsquo;s
+
and &lsquo;Conway&rsquo;s Game of Life&rsquo;.We aim for
-
Game of Life&rsquo;. We aim for these complementary systems to
+
these
-
exhibit
+
complementary systems to exhibit complex intercellular behaviours
-
complex intercellular behaviours arising from the application of simple
+
arising from the application of simple &lsquo;game&rsquo;
-
&lsquo;game&rsquo; rules.</p>
+
rules.
-
<p>John Conway&rsquo;s Game of
+
<p>John
-
Life is a popular representation of
+
Conway&rsquo;s Game of Life is a popular representation of cellular
-
cellular
+
automata. As a zero player game, it proceeds without further input once
automata. As a zero player game, it proceeds without further input once
initiated. We intend to utilize the Game&rsquo;s basic rules to
initiated. We intend to utilize the Game&rsquo;s basic rules to
develop
develop
-
a
+
a system on two separate plasmids that fluoresces in an imitation of
-
system on two separate plasmids that fluoresces in an imitation of the
+
the Game. Thereby we aim to initiate and thus control the creation of
-
Game. Thereby we aim to initiate and thus control the creation of
+
patterns produced by the oscillating bacteria.</p>
-
patterns produced by the oscillating bacteria.<br />
+
 
-
<br />
+
<p>Rock-Paper-Scissors&rsquo;
-
&lsquo;Rock-Paper-Scissors&rsquo; is a well-known two-person
+
is a well-known two-person hand game
-
hand game
+
whereby
-
whereby the
+
the players seek to produce a gesture that will defeat that of their
-
players seek to produce a gesture that will defeat that of their
+
opponent. By coordinating the promoters and operators of three
opponent. By coordinating the promoters and operators of three
different bacterial cells, we have designed a system where each
different bacterial cells, we have designed a system where each
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aim is to have no bias within the system so that the end result is not
aim is to have no bias within the system so that the end result is not
predetermined.</p>
predetermined.</p>
-
<p></p>
+
Learn more about the iGEM
-
<p></p>
+
competition <a href="https://2009.igem.org/About">here</a> and about synthetic biology
-
<h3>Team Mugshot</h3>
+
<a href="http://en.wikipedia.org/wiki/Synthetic_biology">here.</a></h3>
-
<br />
+
-
<img style="width: 650px; height: 359px;"
+
-
alt="mugshot"
+
-
src="http://img401.imageshack.us/img401/7182/su001.jpg" /><br />
+
-
<p>From The Left; Steph, Dani,
+
-
Tom, Jon, Jo, Paul, Karolina, Claire, Rich.</p>
+
-
<br />
+
-
<!-- END CONTENT --> </div>
+
-
<div class="sidebar"><!-- SIDEBAR -->
+
-
<h4>Search &nbsp;&nbsp;<img
+
-
style="width: 94px; height: 37px;" alt=""
+
-
src="http://img196.imageshack.us/img196/9328/googlezgn.jpg" /></h4>
+
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<form method="get"
+
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action="http://www.google.com/search">
+
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+
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style="border: 0px solid black; padding: 4px; width: 25em;">
+
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+
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border="0" cellpadding="0">
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+
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        <td><input
+
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name="q" size="15" maxlength="180" type="text" />
+
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        <input value="Go"
+
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type="submit" /></td>
+
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      </tr>
+
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      <tr>
+
-
        <td style="font-size: 75%;"
+
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align="center"><input
+
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name="sitesearch" value="https://2009.igem.org/Team:Southampton"
+
-
checked="checked" type="checkbox" /> only
+
-
search Southampton iGEM wiki<br />
+
-
        </td>
+
-
      </tr>
+
-
    </tbody>
+
-
  </table>
+
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  </div>
+
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</form>
+
-
<h4>Statistics</h4>
+
-
<br />
+
-
<a
+
-
href="http://www3.clustrmaps.com/counter/maps.php?url=https://2009.igem.org/Team:Southampton"
+
-
id="clustrMapsLink"><img
+
-
src="index_files/index2.jpeg" style="border: 0px none ;"
+
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alt="Locations of visitors to this page"
+
-
title="Locations of visitors to this page" id="clustrMapsImg"
+
-
onerror="this.onerror=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com';" /></a>
+
-
<h4>Links</h4>
+
-
<br />
+
-
<a href="http://www.semibiotics.org"><img
+
-
style="border: 0px solid ; width: 143px; height: 54px;" alt=""
+
-
src="http://img406.imageshack.us/img406/1584/semibio.jpg" /></a><br />
+
-
<br />
+
-
<a
+
-
href="http://www.facebook.com/group.php?gid=107692312882"><img
+
-
style="border: 0px solid ; width: 143px; height: 54px;" alt=""
+
-
src="http://img194.imageshack.us/img194/6637/94031861.jpg" /></a><br />
+
-
<div style="margin-left: 80px;"><br />
+
</div>
</div>
-
<a href="http://www.soton.ac.uk"><img
+
<div id="updateheader">
-
style="border: 0px solid ; width: 143px; height: 54px;" alt=""
+
<h4>Updates</h4>
-
src="http://img222.imageshack.us/img222/1320/soton.jpg" /></a>
+
</div>
</div>
-
<div class="footer">
+
<div id="updatedate">
-
<p>Home | The Team | The Project |
+
<h2>21/09/2009</h2>
-
Biobrick Parts | Modelling |
+
-
Notebook&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;
+
-
&nbsp;&nbsp;&nbsp; &nbsp;
+
-
University of Southampton <span
+
-
style="font-weight: bold;">iGEM</span>
+
-
2009
+
-
&nbsp;&nbsp;&nbsp; <br />
+
-
</p>
+
</div>
</div>
 +
<div id="updatedate2">
 +
<h2></h2>
 +
</div>
</div>
 +
<div id="teamheader">
 +
<h1>Team</h1>
</div>
</div>
 +
<div id="teampic">
 +
<img style="width: 296px; height: 164px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/team.jpg" /></div>
 +
<div id="scene"> <a href="http://www.wessexscene.co.uk/features/3394"><img src="https://static.igem.org/mediawiki/2009/0/04/Scene.png" alt="" width="329" height="108" border="0"/></a></div>
 +
<div id="facebook"> <a href="http://www.facebook.com/home.php?#/group.php?gid=107692312882"><img style="width: 97px; height: 100px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/facebook.jpg" /></a></div>
 +
<div id="teamcaption">
 +
<h6>From left :- Steph, Danie,
 +
Tom, Jon, Jo, Paul, Karolina, Claire, Richmond. </h6>
</div>
</div>
 +
<div id="projfind">
 +
<h6>For more information on our
 +
project, <a href="https://2009.igem.org/Team:Southampton/Project">Click Here.</a></h6>
 +
</div>
 +
 +
<div id="findout">
 +
<h6>To find out more about us, go
 +
to the Team Page
 +
or look us up on <a href="http://www.facebook.com/home.php?#/group.php?gid=107692312882">facebook</a>.</h6>
 +
</div>
 +
<div id="updatecontent"><br><br><br>
 +
<h3>Final week, and what do we have to show for our iGEM experience?
 +
We performed TA cloning on our novel biobricks; pTac and GFP. It is the first (and only) successful ligation so far… <br>
 +
We have a final meeting and farewell with a few refreshments… For more click</h3>
 +
<div id="notebooklink"><a href="https://2009.igem.org/Team:Southampton/Notebook" style="font-family: Arial, Helvetica, sans-serif; font-size: 13px;">here.</a></div>
 +
</div>
 +
</div>
 +
<div id="experiencebox"><a href="https://2009.igem.org/Team:Southampton/HP/igemexper"><img src="https://static.igem.org/mediawiki/2009/9/98/Experience.jpg" width="134" height="105" border="0" /></a></div>
 +
 +
<div id="linksemi"> <a href="http://www.semibiotics.org/"><img style="width: 145px; height: 56px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/sb.jpg" /></a></div>
 +
<div id="linklsi"> <a href="http://www.lsi.soton.ac.uk/"><img style="width: 145px; height: 56px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/lsi.jpg" /></a></div>
 +
<div id="linksoton"> <a href="http://www.soton.ac.uk"><img style="width: 145px; height: 56px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/soton.jpg" /></a></div>
 +
 +
<div id="linkheader">
 +
<h4>Sponsors
 +
</h4>
 +
</div>
 +
 +
<div id="clustr"><a
 +
href="http://www3.clustrmaps.com/counter/maps.php?url=https://2009.igem.org/Team:Southampton"
 +
id="clustrMapsLink"><img
 +
src="http://www3.clustrmaps.com/counter/index2.php?url=https://2009.igem.org/Team:Southampton"
 +
style="border: 0px none ;"
 +
alt="Locations of visitors to this page"
 +
title="Locations of visitors to this page" id="clustrMapsImg">
 +
</a>
 +
<script type="text/javascript">
 +
function cantload() {
 +
img = document.getElementById("clustrMapsImg");
 +
img.onerror = null;
 +
img.src = "http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg";
 +
document.getElementById("clustrMapsLink").href = "http://www2.clustrmaps.com";
 +
}
 +
img = document.getElementById("clustrMapsImg");
 +
img.onerror = cantload;
 +
</script></div>
 +
<div id="logobox2">
 +
<img style="width: 440px; height: 398px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/ecoli.jpg" /></div>
 +
<div id="flickriver"><a
 +
href="http://www.flickriver.com/photos/sotonigem/popular-interesting/">
 +
<img
 +
src="http://www.flickriver.com/badge/user/all/interesting/shuffle/medium-horiz/323232/ebebeb/41427059@N03.jpg"
 +
alt="Southampton iGEM 2009 - View my most interesting photos on Flickriver"
 +
title="Southampton iGEM 2009 - View my most interesting photos on Flickriver"
 +
border="0" /></a></div>
 +
<div id="flickriver2"><a
 +
href="http://www.flickriver.com/photos/sotonigem/">
 +
<img
 +
src="http://www.flickriver.com/badge/user/all/recent/shuffle/medium-horiz/323232/ebebeb/41427059@N03.jpg"
 +
alt="Southampton iGEM 2009 - View my recent photos on Flickriver"
 +
title="Southampton iGEM 2009 - View my recent photos on Flickriver"
 +
border="0" /></a></div>
 +
<div id="blanker">
 +
<img style="width: 238px; height: 23px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/blank.gif" /></div>
 +
<div id="blanker2">
 +
 +
<img style="width: 238px; height: 23px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/blank.gif" /></div>
 +
<div id="sufooter">
 +
<h5>| <a href="#">Top</a> |&nbsp; &nbsp;&nbsp;&nbsp;
 +
&nbsp;&nbsp;&nbsp;
 +
University of
 +
Southampton iGEM 2009</h5>
</div>
</div>
</body>
</body>
</html>
</html>

Latest revision as of 20:04, 21 October 2009

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> University of Southampton Wiki

Project Description

01/08/2009 - Project

As a new team to the iGEM competition, Southampton hopes to bring an innovative application of quorum sensing to the 2009 Jamboree. Using this method of bacterial communication, we have designed two systems, which demonstrate the classic games ‘Rock-Paper-Scissors’ and ‘Conway’s Game of Life’.We aim for these complementary systems to exhibit complex intercellular behaviours arising from the application of simple ‘game’ rules.

John Conway’s Game of Life is a popular representation of cellular automata. As a zero player game, it proceeds without further input once initiated. We intend to utilize the Game’s basic rules to develop a system on two separate plasmids that fluoresces in an imitation of the Game. Thereby we aim to initiate and thus control the creation of patterns produced by the oscillating bacteria.

Rock-Paper-Scissors’ is a well-known two-person hand game whereby the players seek to produce a gesture that will defeat that of their opponent. By coordinating the promoters and operators of three different bacterial cells, we have designed a system where each bacterium can disable the primary plasmid of specific opponent cells, whilst simultaneously enduring the attacks of their rival cells. Our aim is to have no bias within the system so that the end result is not predetermined.

Learn more about the iGEM competition here and about synthetic biology here.

Updates

21/09/2009

Team

From left :- Steph, Danie, Tom, Jon, Jo, Paul, Karolina, Claire, Richmond.
For more information on our project, Click Here.
To find out more about us, go to the Team Page or look us up on facebook.



Final week, and what do we have to show for our iGEM experience? We performed TA cloning on our novel biobricks; pTac and GFP. It is the first (and only) successful ligation so far…
We have a final meeting and farewell with a few refreshments… For more click

Sponsors

Locations of visitors to this page
| Top |          University of Southampton iGEM 2009

Retrieved from "http://2009.igem.org/Team:Southampton"