Team:Southampton

From 2009.igem.org

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<ul class="cssMenu cssMenum">
<ul class="cssMenu cssMenum">
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton">Home</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton">Home</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="#"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Aims">Project Aims</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Intro">Project Introduction</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li>
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         <li class=" cssMenui"><a class="  cssMenui" href="#"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Achievements">Project Achievements</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Projection">Project Projection</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Safety">Project Safety</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Refer">References and Links</a></li>
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         <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1">Protocol Theory</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/ColonyPCR">Colony PCR</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts">Biobrick Parts</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Biobrick Parts<![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team"><span>Team</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Southampton">Southampton Profile</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/University">University Profile</a></li>
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling">Modelling</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling">Modelling Overview</a></li>
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         <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/GoL">Game Of Life</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/Open">Open Access</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP">Overview</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Outreach">Outreach</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/igemexper">iGEM Experience</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Political">Political Awareness</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Legacy">Our Legacy</a></li>
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    <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors"><span>Acknowledgments</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Southampton">Team Southampton</a></li>
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or look us up on <a href="http://www.facebook.com/home.php?#/group.php?gid=107692312882">facebook</a>.</h6>
or look us up on <a href="http://www.facebook.com/home.php?#/group.php?gid=107692312882">facebook</a>.</h6>
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<h5>The competent cells finally arrive and we are all frantically busy in labs again. Has anything been successful yet? No.
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<h3>Final week, and what do we have to show for our iGEM experience?
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One of our post grad supervisors makes some dodgy minimal media. As in, it was cloudy and brown. ‘It’s okay he said, they will set clear…’
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We performed TA cloning on our novel biobricks; pTac and GFP. It is the first (and only) successful ligation so far… <br>
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Meanwhile, our other supervisor threw our ligation products out, only to root around the bin and find that they had kept the right ones in the first place… we look up to our supervisors, we really do. Our first iGEM romance becomes apparent. We’re just surprised it’s taken this long to occur!
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We have a final meeting and farewell with a few refreshments… For more click</h3>
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Our project supervisor returns sporting the most fantastic suit. Just imagine, beige chinos, brown shoes, white shirt, gold tie and black, velvet jacket. Stunning.
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<div id="notebooklink"><a href="https://2009.igem.org/Team:Southampton/Notebook" style="font-family: Arial, Helvetica, sans-serif; font-size: 13px;">here.</a></div>
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<h5>Home | Project | Biobrick
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<h5>| <a href="#">Top</a> |&nbsp; &nbsp;&nbsp;&nbsp;
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Parts | Team | Modelling | Notebook | Sponsors
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University of
University of

Latest revision as of 20:04, 21 October 2009

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> University of Southampton Wiki

Project Description

01/08/2009 - Project

As a new team to the iGEM competition, Southampton hopes to bring an innovative application of quorum sensing to the 2009 Jamboree. Using this method of bacterial communication, we have designed two systems, which demonstrate the classic games ‘Rock-Paper-Scissors’ and ‘Conway’s Game of Life’.We aim for these complementary systems to exhibit complex intercellular behaviours arising from the application of simple ‘game’ rules.

John Conway’s Game of Life is a popular representation of cellular automata. As a zero player game, it proceeds without further input once initiated. We intend to utilize the Game’s basic rules to develop a system on two separate plasmids that fluoresces in an imitation of the Game. Thereby we aim to initiate and thus control the creation of patterns produced by the oscillating bacteria.

Rock-Paper-Scissors’ is a well-known two-person hand game whereby the players seek to produce a gesture that will defeat that of their opponent. By coordinating the promoters and operators of three different bacterial cells, we have designed a system where each bacterium can disable the primary plasmid of specific opponent cells, whilst simultaneously enduring the attacks of their rival cells. Our aim is to have no bias within the system so that the end result is not predetermined.

Learn more about the iGEM competition here and about synthetic biology here.

Updates

21/09/2009

Team

From left :- Steph, Danie, Tom, Jon, Jo, Paul, Karolina, Claire, Richmond.
For more information on our project, Click Here.
To find out more about us, go to the Team Page or look us up on facebook.



Final week, and what do we have to show for our iGEM experience? We performed TA cloning on our novel biobricks; pTac and GFP. It is the first (and only) successful ligation so far…
We have a final meeting and farewell with a few refreshments… For more click

Sponsors

Locations of visitors to this page
| Top |          University of Southampton iGEM 2009

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