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Latest revision as of 20:43, 21 October 2009

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> University of Southampton Wiki

Biobrick Parts: Game of Life

For a list of all our parts, please click here.

To view the parts used in our Rock Paper Scissors sub-project, click here.

Part Name	Type	Description	Team Favourite?	Length
K180000	Regulatory	Hybrid promoter (trp & lac regulated – tac pR) A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.	Yes	1447
K180001	Coding	GFP-LVA fluorescent protein GFPmut3b (green fluorescent protein) with an LVA degradation tag for shortened fluorescence compared to part E0040.	Yes	754
K180002	Composite	tac pR testing plasmid (GFP) Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.	Yes	2330
K180004	Composite	Game of Life – Primary Plasmid Primary plasmid for Game of Life. Must be used in conjunction with part K180007.	Yes	3138
K180007	Composite	Game of Life – Secondary Plasmid [tac pR, LuxI generator] Secondary plasmid for Game of Life; generates LuxI under tac pR regulation. Must be used in conjunction with K180004.	Yes	2195

K180003	Composite	tac pR testing plasmid (GFP) – basic Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.		2173
K180005	Composite	Game of Life – Primary plasmid (part 1) [LuxR generator] Part of the Game of Life primary plasmid; constitutively produces LuxR.		979
K180006	Composite	Game of Life – Primary plasmid (part 2) [lux pR, GFP and LacI generator] Part of the Game of Life primary plasmid; generates GFP and LacI under lux pR regulation.		2151

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-<h1>Biobrick Parts: Rock Paper Scissors</h1>
+<h1>Biobrick Parts: Game of Life</h1>
 <h4>&nbsp;</h4>
 </div>
@@ Line 164: / Line 164: @@
        <td colspan="2" align="left" valign="top" bgcolor="#5D5D5D"><h3>For  a list of all our parts, please click <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&amp;group=Southampton&amp;Done=1">here</a>.</h3>
          <h3><br />
-           To  view the parts used in our Game of Life sub-project, click <a href="https://2009.igem.org/Team:Southampton/Parts/GoL">here</a>.</h3>
+           To  view the parts used in our Rock Paper Scissors sub-project, click <a href="https://2009.igem.org/Team:Southampton/Parts/RPS">here</a>.</h3>
          <p>&nbsp;</p>
          <table width="95%" border="0" align="center" cellpadding="0" cellspacing="0">
@@ Line 177: / Line 177: @@
              <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180000">K180000</a></h3></td>
              <td width="98" bgcolor="#5D5D5D"><h3 align="center">Regulatory<br />
-            <img src="https://static.igem.org/mediawiki/2009/1/1f/Clip_image002_0004.jpg" alt="" width="49" height="33" border="0" /></h3></td>
+              <img src="https://static.igem.org/mediawiki/2009/1/1f/Clip_image002_0004.jpg" alt="" width="49" height="33" border="0" /></h3></td>
              <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Hybrid    promoter (trp &amp; lac regulated &ndash; tac pR)</strong><br />
-            A functional, hybrid promoter, derived from the trp and    lac promoters, regulated by trp and lac. tac pR has a stronger binding    affinity than either of its parent promoters.</h3></td>
+              A functional, hybrid promoter, derived from the trp and    lac promoters, regulated by trp and lac. tac pR has a stronger binding    affinity than either of its parent promoters.</h3></td>
-             <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
+             <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes </h3></td>
              <td width="72" bgcolor="#5D5D5D"><h3 align="right">1447</h3></td>
+          </tr>
+          <tr>
+            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180001">K180001</a></h3></td>
+            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Coding<br />
+              <img src="https://static.igem.org/mediawiki/2009/9/91/Clip_image007_0000.jpg" alt="" width="49" height="33" border="0" /></h3></td>
+            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>GFP-LVA fluorescent protein</strong><br />
+              GFPmut3b (green fluorescent protein) with an LVA    degradation tag for shortened fluorescence compared to part <a href="http://partsregistry.org/Part:BBa_E0040">E0040</a>.</h3></td>
+            <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
+            <td width="72" bgcolor="#5D5D5D"><h3 align="right">754</h3></td>
            </tr>
            <tr>
              <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180002">K180002</a></h3></td>
              <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
+              <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
              <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>tac pR    testing plasmid (GFP)</strong><br />
-            Ptac promoter testing plasmid &ndash; green fluorescent    protein is produced if IPTG successfully induces the promoter.</h3></td>
+              Ptac promoter testing plasmid &ndash; green fluorescent    protein is produced if IPTG successfully induces the promoter.</h3></td>
              <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
              <td width="72" bgcolor="#5D5D5D"><h3 align="right">2330</h3></td>
            </tr>
            <tr>
-             <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180010">K180010</a></h3></td>
+             <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180004">K180004</a></h3></td>
              <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
+              <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-             <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors    &ndash; Rock primary plasmid</strong><br />
+             <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Game of    Life &ndash; Primary Plasmid</strong><br />
-            Primary plasmid for rock part of rock-paper-scissors    game. Must be used in conjunction with part <a href="http://partsregistry.org/Part:BBa_K180013">K180013</a>.</h3></td>
+              Primary plasmid for Game of Life. Must be used in    conjunction with part <a href="http://partsregistry.org/Part:BBa_K180007">K180007</a>.</h3></td>
              <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
-             <td width="72" bgcolor="#5D5D5D"><h3 align="right">3888</h3></td>
+             <td width="72" bgcolor="#5D5D5D"><h3 align="right">3138</h3></td>
            </tr>
            <tr>
-             <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180013">K180013</a></h3></td>
+             <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180007">K180007</a></h3></td>
              <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
+              <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-             <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors    &ndash; Rock secondary plasmid</strong><br />
+             <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Game of    Life &ndash; Secondary Plasmid</strong><br />
-               <strong>[rhl pR,    LacI generator]</strong><br />
+               <strong>[tac pR,    LuxI generator]</strong><br />
-            Secondary plasmid for rock part of rock-paper-scissors    game. Must be used in conjunction with part <a href="http://partsregistry.org/Part:BBa_K180010">K180010</a>.</h3></td>
+              Secondary plasmid for Game of Life; generates LuxI    under tac pR regulation. Must be used in conjunction with <a href="http://partsregistry.org/Part:BBa_K180004">K180004</a>.</h3></td>
              <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
-             <td width="72" bgcolor="#5D5D5D"><h3 align="right">1369</h3></td>
+             <td width="72" bgcolor="#5D5D5D"><h3 align="right">2195</h3></td>
-          </tr>
-          <tr>
-            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180014">K180014</a></h3></td>
-            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors    &ndash; Paper primary plasmid</strong><br />
-            Primary plasmid for paper part of rock-paper-scissors    game. Must be used in conjunction with <a href="http://partsregistry.org/Part:BBa_K180016">K180016</a>.</h3></td>
-            <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes</h3></td>
-            <td width="72" bgcolor="#5D5D5D"><h3 align="right">3952</h3></td>
-          </tr>
-          <tr>
-            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180016">K180016</a></h3></td>
-            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors    &ndash; Paper secondary plasmid</strong><br />
-              <strong>[lux pR,    LacI generator]</strong><br />
-            Secondary plasmid for paper part of rock-paper-scissors    game. Must be used in conjunction with <a href="http://partsregistry.org/Part:BBa_K180014">K180014</a>.</h3></td>
-            <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes </h3></td>
-            <td width="72" bgcolor="#5D5D5D"><h3 align="right">1371</h3></td>
-          </tr>
-          <tr>
-            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180017">K180017</a></h3></td>
-            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors    &ndash; Scissors primary plasmid</strong><br />
-            Primary plasmid for scissors part of    rock-paper-scissors game. Must be used in conjunction with <a href="http://partsregistry.org/Part:BBa_K180020">K180020</a>.</h3></td>
-            <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes </h3></td>
-            <td width="72" bgcolor="#5D5D5D"><h3 align="right">3858</h3></td>
-          </tr>
-          <tr>
-            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180020">K180020</a></h3></td>
-            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock-paper-scissors    &ndash; Scissors secondary plasmid </strong><br />
-              <strong>[las pR,    LacI generator]</strong><br />
-            Primary plasmid for scissors part of    rock-paper-scissors game. Must be used in conjunction with <a href="http://partsregistry.org/Part:BBa_K180017">K180017</a>.</h3></td>
-            <td width="164" bgcolor="#5D5D5D"><h3 align="center">Yes </h3></td>
-            <td width="72" bgcolor="#5D5D5D"><h3 align="right">1473</h3></td>
            </tr>
            <tr>
@@ Line 259: / Line 230: @@
              <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180003">K180003</a></h3></td>
              <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
+              <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
              <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>tac pR    testing plasmid (GFP) &ndash; basic</strong><br />
-            Basic version of <a href="http://partsregistry.org/Part:BBa_K180002">K180002</a>; no RBS or stop    BioBrick parts. Allows to see the efficiency of GFP production with no RBS    preceding the GFP sequence. </h3></td>
+              Basic version of <a href="http://partsregistry.org/Part:BBa_K180002">K180002</a>; no RBS or stop    BioBrick parts. Allows to see the efficiency of GFP production with no RBS    preceding the GFP sequence. </h3></td>
              <td width="164" bgcolor="#5D5D5D"><h3 align="center">&nbsp;</h3></td>
              <td width="72" bgcolor="#5D5D5D"><h3 align="right">2173</h3></td>
@@ Line 268: / Line 239: @@
              <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180005">K180005</a></h3></td>
              <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
+              <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-             <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Paper &ndash;    Primary plasmid (part 1)</strong><br />
+             <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Game of    Life &ndash; Primary plasmid (part 1)</strong><br />
                <strong>[LuxR    generator]</strong><br />
-            Part of the paper primary plasmid; constitutively    produces LuxR.</h3></td>
+              Part of the Game of Life primary plasmid;    constitutively produces LuxR.</h3></td>
              <td width="164" bgcolor="#5D5D5D"><h3 align="center">&nbsp;</h3></td>
              <td width="72" bgcolor="#5D5D5D"><h3 align="right">979</h3></td>
            </tr>
            <tr>
-             <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180008">K180008</a></h3></td>
+             <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180006">K180006</a></h3></td>
-            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Coding<br />
-            <img src="https://static.igem.org/mediawiki/2009/9/91/Clip_image007_0000.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>mCherry    (rights owned by Clontech)</strong><br />
-            mCherry fluorescent protein &ndash; a derivative of the DsRed    fluorescent protein with a short maturation time but lower relative    brightness.</h3></td>
-            <td width="164" bgcolor="#5D5D5D"><h3 align="center">&nbsp;</h3></td>
-            <td width="72" bgcolor="#5D5D5D"><h3 align="right">708</h3></td>
-          </tr>
-          <tr>
-            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180009">K180009</a></h3></td>
-            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Coding<br />
-            <img src="https://static.igem.org/mediawiki/2009/9/91/Clip_image007_0000.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>mBanana    (rights owned by Clontech)</strong><br />
-            mBanana fluorescent protein &ndash; a derivative of the DsRed    fluorescent protein with a longer maturation time and lower relative    brightness than <a href="http://partsregistry.org/Part:BBa_K180008">mCherry</a>.</h3></td>
-            <td width="164" bgcolor="#5D5D5D"><h3 align="center">&nbsp;</h3></td>
-            <td width="72" bgcolor="#5D5D5D"><h3 align="right">708</h3></td>
-          </tr>
-          <tr>
-            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180011">K180011</a></h3></td>
              <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
+              <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-             <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock &ndash;    Primary plasmid (part 1)</strong><br />
+             <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Game of    Life &ndash; Primary plasmid (part 2)</strong><br />
-               <strong>[RhlR    generator]</strong><br />
+               <strong>[lux pR,    GFP and LacI generator]</strong><br />
-            Part of the rock primary plasmid; constitutively    produces RhlR.</h3></td>
+              Part of the Game of Life primary plasmid; generates GFP    and LacI under lux pR regulation.</h3></td>
              <td width="164" bgcolor="#5D5D5D"><h3 align="center">&nbsp;</h3></td>
-             <td width="72" bgcolor="#5D5D5D"><h3 align="right">927</h3></td>
+             <td width="72" bgcolor="#5D5D5D"><h3 align="right">2151</h3></td>
-          </tr>
-          <tr>
-            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180012">K180012</a></h3></td>
-            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Rock &ndash;    Primary plasmid (part 2)</strong><br />
-              <strong>[tac pR,    mCherry and LasI generator]</strong><br />
-            Part of the rock primary plasmid; generates mCherry and    LasI under tac pR regulation.</h3></td>
-            <td width="164" bgcolor="#5D5D5D"><h3 align="center">&nbsp;</h3></td>
-            <td width="72" bgcolor="#5D5D5D"><h3 align="right">2953</h3></td>
-          </tr>
-          <tr>
-            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180015">K180015</a></h3></td>
-            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Paper &ndash;    Primary plasmid (part 2)</strong><br />
-              <strong>[tac pR,    GFP and RhlI generator]</strong><br />
-            Part of the paper primary plasmid; generates GFP and    RhlI under tac pR regulation.</h3></td>
-            <td width="164" bgcolor="#5D5D5D"><h3 align="center">&nbsp;</h3></td>
-            <td width="72" bgcolor="#5D5D5D"><h3 align="right">2965</h3></td>
-          </tr>
-          <tr>
-            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180018">K180018</a></h3></td>
-            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Scissors    &ndash; Primary plasmid (part 1)</strong><br />
-              <strong>[LasR    generator]</strong><br />
-            Part of the scissors primary plasmid; constitutively    produces LasR.</h3></td>
-            <td width="164" valign="top" bgcolor="#5D5D5D"><h3>&nbsp;</h3></td>
-            <td width="72" bgcolor="#5D5D5D"><h3 align="right">921</h3></td>
-          </tr>
-          <tr>
-            <td width="108" bgcolor="#5D5D5D"><h3><a href="http://partsregistry.org/Part:BBa_K180019">K180019</a></h3></td>
-            <td width="98" bgcolor="#5D5D5D"><h3 align="center">Composite<br />
-            <img src="https://static.igem.org/mediawiki/2009/f/fc/Clip_image004_0003.jpg" alt="" width="49" height="33" border="0" /></h3></td>
-            <td width="509" valign="top" bgcolor="#5D5D5D"><h3><strong>Scissors    &ndash; Primary plasmid (part 2)</strong><br />
-              <strong>[tac pR,    mBanana and LuxI generator]</strong><br />
-            Part of the scissors primary plasmid; generates mBanana    and LuxI under tac pR regulation.</h3></td>
-            <td width="164" valign="top" bgcolor="#5D5D5D"><h3>&nbsp;</h3></td>
-            <td width="72" bgcolor="#5D5D5D"><h3 align="right">2929</h3></td>
            </tr>
-      </table><h3>&nbsp;</h3></td>
+        </table>
+      <p>&nbsp;</p><h3>&nbsp;</h3></td>
      </tr>
    </table>

Team:Southampton/Parts/GoL

From 2009.igem.org

Latest revision as of 20:43, 21 October 2009

Biobrick Parts: Game of Life

For a list of all our parts, please click here.

To view the parts used in our Rock Paper Scissors sub-project, click here.

Part Name

Type

Description

Team Favourite?

Length

Regulatory

Hybrid promoter (trp & lac regulated – tac pR) A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.

Yes

1447

Coding

GFP-LVA fluorescent protein GFPmut3b (green fluorescent protein) with an LVA degradation tag for shortened fluorescence compared to part E0040.

Yes

754

Composite

tac pR testing plasmid (GFP) Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.

Yes

2330

Composite

Game of Life – Primary Plasmid Primary plasmid for Game of Life. Must be used in conjunction with part K180007.

Yes

3138

Composite

Game of Life – Secondary Plasmid [tac pR, LuxI generator] Secondary plasmid for Game of Life; generates LuxI under tac pR regulation. Must be used in conjunction with K180004.

Yes

2195

Composite

tac pR testing plasmid (GFP) – basic Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.

2173

Composite

Game of Life – Primary plasmid (part 1) [LuxR generator] Part of the Game of Life primary plasmid; constitutively produces LuxR.

979

Composite

Game of Life – Primary plasmid (part 2) [lux pR, GFP and LacI generator] Part of the Game of Life primary plasmid; generates GFP and LacI under lux pR regulation.

2151

| Top | University of Southampton iGEM 2009

Hybrid promoter (trp & lac regulated – tac pR)
A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.

GFP-LVA fluorescent protein
GFPmut3b (green fluorescent protein) with an LVA degradation tag for shortened fluorescence compared to part E0040.

tac pR testing plasmid (GFP)
Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.

Game of Life – Primary Plasmid
Primary plasmid for Game of Life. Must be used in conjunction with part K180007.

Game of Life – Secondary Plasmid
[tac pR, LuxI generator]
Secondary plasmid for Game of Life; generates LuxI under tac pR regulation. Must be used in conjunction with K180004.

tac pR testing plasmid (GFP) – basic
Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.

Game of Life – Primary plasmid (part 1)
[LuxR generator]
Part of the Game of Life primary plasmid; constitutively produces LuxR.

Game of Life – Primary plasmid (part 2)
[lux pR, GFP and LacI generator]
Part of the Game of Life primary plasmid; generates GFP and LacI under lux pR regulation.