Team:Southampton

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<head>
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  <meta content="text/html; charset=ISO-8859-1"
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  <title>University of Southampton
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Wiki</title>
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<ul class="cssMenu cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton">Home</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Aims">Project Aims</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Intro">Project Introduction</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Achievements">Project Achievements</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Projection">Project Projection</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Safety">Project Safety</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Refer">References and Links</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1">Protocol Theory</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/ColonyPCR">Colony PCR</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/DoubleDigestion">DNA Double Digestion</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Gel">Gel Electrophoresis</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Hfpcr">High Fidelity PCR</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/LBplates">LB Plates</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligation">Ligation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Biobrick Parts<![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li>
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</ul></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team"><span>Team</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Southampton">Southampton Profile</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/University">University Profile</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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            <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling"><span>Modelling</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling">Modelling Overview</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/GoL">Game Of Life</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/RPS">Rock Paper Scissors</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/Open">Open Access</a></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP"><span>Human Practices</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP">Overview</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Outreach">Outreach</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/igemexper">iGEM Experience</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Political">Political Awareness</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Legacy">Our Legacy</a></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook">Notebook</a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<html>
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook">Notebook</a></li>
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<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook/Labbook">Lab Book</a></li>
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Notebook/Advisorblog">Advisor Blog</a></li>
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This is a template page. READ THESE INSTRUCTIONS.
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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    <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors"><span>Acknowledgments</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Sponsors">Sponsors</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Southampton">Team Southampton</a></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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</ul>
</div>
</div>
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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<div id="bg"><img style="width: 975px; height: 1005px;" id="bg"
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
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src="http://www.personal.soton.ac.uk/jeh2v07/images/BGHOME.gif" alt="" /></div>
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<div id="logobox">
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<img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /></div>
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<div id="projectheading">
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<h1>Project Description</h1>
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</div>
</div>
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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<div id="bluesubtitle">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook.  PLEASE keep all of your pages within your teams namespace. 
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<h2>01/08/2009 - Project</h2>
</div>
</div>
 +
<div id="projdesc">
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<h3>As a new team to the iGEM
 +
competition, Southampton hopes to bring
 +
an innovative application of quorum sensing to the 2009 Jamboree. Using
 +
this method of bacterial communication, we have designed two systems,
 +
which demonstrate the classic games
 +
&lsquo;Rock-Paper-Scissors&rsquo;
 +
and &lsquo;Conway&rsquo;s Game of Life&rsquo;.We aim for
 +
these
 +
complementary systems to exhibit complex intercellular behaviours
 +
arising from the application of simple &lsquo;game&rsquo;
 +
rules.
 +
<p>John
 +
Conway&rsquo;s Game of Life is a popular representation of cellular
 +
automata. As a zero player game, it proceeds without further input once
 +
initiated. We intend to utilize the Game&rsquo;s basic rules to
 +
develop
 +
a system on two separate plasmids that fluoresces in an imitation of
 +
the Game. Thereby we aim to initiate and thus control the creation of
 +
patterns produced by the oscillating bacteria.</p>
 +
 +
<p>Rock-Paper-Scissors&rsquo;
 +
is a well-known two-person hand game
 +
whereby
 +
the players seek to produce a gesture that will defeat that of their
 +
opponent. By coordinating the promoters and operators of three
 +
different bacterial cells, we have designed a system where each
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bacterium can disable the primary plasmid of specific opponent cells,
 +
whilst simultaneously enduring the attacks of their rival cells. Our
 +
aim is to have no bias within the system so that the end result is not
 +
predetermined.</p>
 +
Learn more about the iGEM
 +
competition <a href="https://2009.igem.org/About">here</a> and about synthetic biology
 +
<a href="http://en.wikipedia.org/wiki/Synthetic_biology">here.</a></h3>
</div>
</div>
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</html>
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<div id="updateheader">
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<h4>Updates</h4>
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</div>
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<div id="updatedate">
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<h2>21/09/2009</h2>
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</div>
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<div id="updatedate2">
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<h2></h2>
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<!-- *** End of the alert box *** -->
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</div>
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<div id="teamheader">
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<h1>Team</h1>
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</div>
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<div id="teampic">
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<img style="width: 296px; height: 164px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/team.jpg" /></div>
 +
<div id="scene"> <a href="http://www.wessexscene.co.uk/features/3394"><img src="https://static.igem.org/mediawiki/2009/0/04/Scene.png" alt="" width="329" height="108" border="0"/></a></div>
 +
<div id="facebook"> <a href="http://www.facebook.com/home.php?#/group.php?gid=107692312882"><img style="width: 97px; height: 100px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/facebook.jpg" /></a></div>
 +
<div id="teamcaption">
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<h6>From left :- Steph, Danie,
 +
Tom, Jon, Jo, Paul, Karolina, Claire, Richmond. </h6>
 +
</div>
 +
<div id="projfind">
 +
<h6>For more information on our
 +
project, <a href="https://2009.igem.org/Team:Southampton/Project">Click Here.</a></h6>
 +
</div>
 +
<div id="findout">
 +
<h6>To find out more about us, go
 +
to the Team Page
 +
or look us up on <a href="http://www.facebook.com/home.php?#/group.php?gid=107692312882">facebook</a>.</h6>
 +
</div>
 +
<div id="updatecontent"><br><br><br>
 +
<h3>Final week, and what do we have to show for our iGEM experience?
 +
We performed TA cloning on our novel biobricks; pTac and GFP. It is the first (and only) successful ligation so far… <br>
 +
We have a final meeting and farewell with a few refreshments… For more click</h3>
 +
<div id="notebooklink"><a href="https://2009.igem.org/Team:Southampton/Notebook" style="font-family: Arial, Helvetica, sans-serif; font-size: 13px;">here.</a></div>
 +
</div>
 +
</div>
 +
<div id="experiencebox"><a href="https://2009.igem.org/Team:Southampton/HP/igemexper"><img src="https://static.igem.org/mediawiki/2009/9/98/Experience.jpg" width="134" height="105" border="0" /></a></div>
 +
<div id="linksemi"> <a href="http://www.semibiotics.org/"><img style="width: 145px; height: 56px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/sb.jpg" /></a></div>
 +
<div id="linklsi"> <a href="http://www.lsi.soton.ac.uk/"><img style="width: 145px; height: 56px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/lsi.jpg" /></a></div>
 +
<div id="linksoton"> <a href="http://www.soton.ac.uk"><img style="width: 145px; height: 56px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/soton.jpg" /></a></div>
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{|align="justify"
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<div id="linkheader">
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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<h4>Sponsors
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|[[Image:Example_logo.png|200px|right|frame]]
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</h4>
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|-
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</div>
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|
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Team.png|right|frame|Your team picture]]
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|-
+
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|
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|align="center"|[[Team:Southampton | Team Example]]
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|}
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<!--- The Mission, Experiments --->
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<div id="clustr"><a
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 +
id="clustrMapsLink"><img
 +
src="http://www3.clustrmaps.com/counter/index2.php?url=https://2009.igem.org/Team:Southampton"
 +
style="border: 0px none ;"
 +
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 +
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 +
</a>
 +
<script type="text/javascript">
 +
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 +
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 +
<div id="logobox2">
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<img style="width: 440px; height: 398px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/ecoli.jpg" /></div>
 +
<div id="flickriver"><a
 +
href="http://www.flickriver.com/photos/sotonigem/popular-interesting/">
 +
<img
 +
src="http://www.flickriver.com/badge/user/all/interesting/shuffle/medium-horiz/323232/ebebeb/41427059@N03.jpg"
 +
alt="Southampton iGEM 2009 - View my most interesting photos on Flickriver"
 +
title="Southampton iGEM 2009 - View my most interesting photos on Flickriver"
 +
border="0" /></a></div>
 +
<div id="flickriver2"><a
 +
href="http://www.flickriver.com/photos/sotonigem/">
 +
<img
 +
src="http://www.flickriver.com/badge/user/all/recent/shuffle/medium-horiz/323232/ebebeb/41427059@N03.jpg"
 +
alt="Southampton iGEM 2009 - View my recent photos on Flickriver"
 +
title="Southampton iGEM 2009 - View my recent photos on Flickriver"
 +
border="0" /></a></div>
 +
<div id="blanker">
 +
<img style="width: 238px; height: 23px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/blank.gif" /></div>
 +
<div id="blanker2">
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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<img style="width: 238px; height: 23px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/blank.gif" /></div>
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!align="center"|[[Team:Southampton|Home]]
+
<div id="sufooter">
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!align="center"|[[Team:Southampton/Team|The Team]]
+
<h5>| <a href="#">Top</a> |&nbsp; &nbsp;&nbsp;&nbsp;
-
!align="center"|[[Team:Southampton/Project|The Project]]
+
&nbsp;&nbsp;&nbsp;
-
!align="center"|[[Team:Southampton/Parts|Parts Submitted to the Registry]]
+
University of
-
!align="center"|[[Team:Southampton/Modeling|Modeling]]
+
Southampton iGEM 2009</h5>
-
!align="center"|[[Team:Southampton/Notebook|Notebook]]
+
</div>
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|}
+
</body>
-
(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
+
</html>

Latest revision as of 20:04, 21 October 2009

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> University of Southampton Wiki

Project Description

01/08/2009 - Project

As a new team to the iGEM competition, Southampton hopes to bring an innovative application of quorum sensing to the 2009 Jamboree. Using this method of bacterial communication, we have designed two systems, which demonstrate the classic games ‘Rock-Paper-Scissors’ and ‘Conway’s Game of Life’.We aim for these complementary systems to exhibit complex intercellular behaviours arising from the application of simple ‘game’ rules.

John Conway’s Game of Life is a popular representation of cellular automata. As a zero player game, it proceeds without further input once initiated. We intend to utilize the Game’s basic rules to develop a system on two separate plasmids that fluoresces in an imitation of the Game. Thereby we aim to initiate and thus control the creation of patterns produced by the oscillating bacteria.

Rock-Paper-Scissors’ is a well-known two-person hand game whereby the players seek to produce a gesture that will defeat that of their opponent. By coordinating the promoters and operators of three different bacterial cells, we have designed a system where each bacterium can disable the primary plasmid of specific opponent cells, whilst simultaneously enduring the attacks of their rival cells. Our aim is to have no bias within the system so that the end result is not predetermined.

Learn more about the iGEM competition here and about synthetic biology here.

Updates

21/09/2009

Team

From left :- Steph, Danie, Tom, Jon, Jo, Paul, Karolina, Claire, Richmond.
For more information on our project, Click Here.
To find out more about us, go to the Team Page or look us up on facebook.



Final week, and what do we have to show for our iGEM experience? We performed TA cloning on our novel biobricks; pTac and GFP. It is the first (and only) successful ligation so far…
We have a final meeting and farewell with a few refreshments… For more click

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