Team:Southampton

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   <title>University of Southampton
   <title>University of Southampton
Wiki</title>
Wiki</title>
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<div id="banner"><img style="width: 975px; height: 276px;" id="banner"
<div id="banner"><img style="width: 975px; height: 276px;" id="banner"
  src="http://www.personal.soton.ac.uk/jeh2v07/images/blue.jpg" alt="" /></div>
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<div id="toolbar">
<div id="toolbar">
<ul class="cssMenu cssMenum">
<ul class="cssMenu cssMenum">
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  <li class="cssMenui"><a class="cssMenui"
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton">Home</a></li>
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  href="https://2009.igem.org/Team:Southampton">Home</a></li>
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<li class=" cssMenui"><a class=" cssMenui" href="https://2009.igem.org/Team:Southampton/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Aims">Project Aims</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Intro">Project Introduction</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project">Project Description</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Achievements">Project Achievements</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Projection">Project Projection</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Safety">Project Safety</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Refer">References and Links</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols"><span>Protocols</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols">Protocol Overview</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Theory1">Protocol Theory</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Transformation">Biobrick Transformation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/ColonyPCR">Colony PCR</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/DoubleDigestion">DNA Double Digestion</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Gel">Gel Electrophoresis</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Hfpcr">High Fidelity PCR</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/LBplates">LB Plates</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligation">Ligation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li>
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li>
 +
</ul>
 +
<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Biobrick Parts<![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
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<ul class=" cssMenum">
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li>
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</ul></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team"><span>Team</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
 +
<ul class=" cssMenum">
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Southampton">Southampton Profile</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/University">University Profile</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]--></li>
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 +
            <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling"><span>Modelling</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]-->
 +
<ul class=" cssMenum">
 +
<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling">Modelling Overview</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/GoL">Game Of Life</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/RPS">Rock Paper Scissors</a></li>
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        <li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/Modeling/Open">Open Access</a></li>
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<li class=" cssMenui"><a class="  cssMenui" href="https://2009.igem.org/Team:Southampton/HP/Legacy">Our Legacy</a></li>
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predetermined.</p>
predetermined.</p>
Learn more about the iGEM
Learn more about the iGEM
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competition here and about synthetic biology
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competition <a href="https://2009.igem.org/About">here</a> and about synthetic biology
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here or here.</h3>
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<a href="http://en.wikipedia.org/wiki/Synthetic_biology">here.</a></h3>
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<h2>21/09/2009</h2>
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<div id="scene"> <a href="http://www.wessexscene.co.uk/features/3394"><img src="https://static.igem.org/mediawiki/2009/0/04/Scene.png" alt="" width="329" height="108" border="0"/></a></div>
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<h6>From left :- Steph, Danie,
<h6>From left :- Steph, Danie,
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<h6>For more information on our
<h6>For more information on our
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project, Click Here.</h6>
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project, <a href="https://2009.igem.org/Team:Southampton/Project">Click Here.</a></h6>
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<h6>To find out more about us, go
<h6>To find out more about us, go
to the Team Page
to the Team Page
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or look us up on facebook.</h6>
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or look us up on <a href="http://www.facebook.com/home.php?#/group.php?gid=107692312882">facebook</a>.</h6>
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<h6>We perfect a pattern:
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<h3>Final week, and what do we have to show for our iGEM experience?
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Digest&hellip;ligate&hellip;transform
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We performed TA cloning on our novel biobricks; pTac and GFP. It is the first (and only) successful ligation so far… <br>
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Digest&hellip;ligate&hellip;transform
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We have a final meeting and farewell with a few refreshments… For more click</h3>
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Digest&hellip;ligate&hellip;transform
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<div id="notebooklink"><a href="https://2009.igem.org/Team:Southampton/Notebook" style="font-family: Arial, Helvetica, sans-serif; font-size: 13px;">here.</a></div>
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Shame none of them worked&hellip;
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Digest&hellip;ligate&hellip;transform
 
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Digest&hellip;ligate&hellip;transform
 
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Digest&hellip;ligate&hellip;transform
 
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Shame none of them worked&hellip;
 
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On the plus side, our simulations are looking pretty good!
 
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</h6>
 
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<h5>Home | Project | Biobrick
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<h5>| <a href="#">Top</a> |&nbsp; &nbsp;&nbsp;&nbsp;
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Parts | Team | Modelling | Notebook | Sponsors
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University of
University of

Latest revision as of 20:04, 21 October 2009

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> University of Southampton Wiki

Project Description

01/08/2009 - Project

As a new team to the iGEM competition, Southampton hopes to bring an innovative application of quorum sensing to the 2009 Jamboree. Using this method of bacterial communication, we have designed two systems, which demonstrate the classic games ‘Rock-Paper-Scissors’ and ‘Conway’s Game of Life’.We aim for these complementary systems to exhibit complex intercellular behaviours arising from the application of simple ‘game’ rules.

John Conway’s Game of Life is a popular representation of cellular automata. As a zero player game, it proceeds without further input once initiated. We intend to utilize the Game’s basic rules to develop a system on two separate plasmids that fluoresces in an imitation of the Game. Thereby we aim to initiate and thus control the creation of patterns produced by the oscillating bacteria.

Rock-Paper-Scissors’ is a well-known two-person hand game whereby the players seek to produce a gesture that will defeat that of their opponent. By coordinating the promoters and operators of three different bacterial cells, we have designed a system where each bacterium can disable the primary plasmid of specific opponent cells, whilst simultaneously enduring the attacks of their rival cells. Our aim is to have no bias within the system so that the end result is not predetermined.

Learn more about the iGEM competition here and about synthetic biology here.

Updates

21/09/2009

Team

From left :- Steph, Danie, Tom, Jon, Jo, Paul, Karolina, Claire, Richmond.
For more information on our project, Click Here.
To find out more about us, go to the Team Page or look us up on facebook.



Final week, and what do we have to show for our iGEM experience? We performed TA cloning on our novel biobricks; pTac and GFP. It is the first (and only) successful ligation so far…
We have a final meeting and farewell with a few refreshments… For more click

Sponsors

Locations of visitors to this page
| Top |          University of Southampton iGEM 2009

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