Team:Southampton/Parts/GoL

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Biobrick Parts: Game of Life

 

 

 

 

For a list of all our parts, please click here.


To view the parts used in our Rock Paper Scissors sub-project, click here.

 

Part Name

Type

Description

Team Favourite?

Length

K180000

Regulatory

Hybrid promoter (trp & lac regulated – tac pR)
A functional, hybrid promoter, derived from the trp and lac promoters, regulated by trp and lac. tac pR has a stronger binding affinity than either of its parent promoters.

Yes

1447

K180001

Coding

tac pR testing plasmid (GFP)
GFPmut3b (green fluorescent protein) with an LVA degradation tag for shortened fluorescence compared to part E0040.

Yes

754

K180002

Composite

tac pR testing plasmid (GFP)
Ptac promoter testing plasmid – green fluorescent protein is produced if IPTG successfully induces the promoter.

Yes

2330

K180004

Composite

Game of Life – Primary Plasmid
Primary plasmid for Game of Life. Must be used in conjunction with part K180007.

Yes

3138

K180007

Composite

Game of Life – Secondary Plasmid
[tac pR, LuxI generator]
Secondary plasmid for Game of Life; generates LuxI under tac pR regulation. Must be used in conjunction with K180004.

Yes

2195

 

 

 

 

 

K180003

Composite

tac pR testing plasmid (GFP) – basic
Basic version of K180002; no RBS or stop BioBrick parts. Allows to see the efficiency of GFP production with no RBS preceding the GFP sequence.

 

2173

K180005

Composite

Game of Life – Primary plasmid (part 1)
[LuxR generator]
Part of the Game of Life primary plasmid; constitutively produces LuxR.

 

979

K180006

Composite

Game of Life – Primary plasmid (part 2)
[lux pR, GFP and LacI generator]
Part of the Game of Life primary plasmid; generates GFP and LacI under lux pR regulation.

 

2151

 

 
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