Team:Groningen/Modelling/Characterization

From 2009.igem.org

(Difference between revisions)
(A little more independent of varsToMutate)
Line 91: Line 91:
<script type="text/javascript" src="/Team:Groningen/Modelling/Model.js?action=raw"></script>
<script type="text/javascript" src="/Team:Groningen/Modelling/Model.js?action=raw"></script>
<script type="text/javascript">
<script type="text/javascript">
-
var experiments = [{constants:{Vc:0.0073e-3,Vs:(1.1-0.0073)*1e-3,beta4:0},AsT:10e-6*1e-3,
+
var experiments = [{constants:{Vc:0.0073,Vs:(1.1-0.0073),beta4:0},AsT:10e-6,
                     AsinT:[101.917808219178e-6,394.520547945205e-6,723.287671232877e-6,
                     AsinT:[101.917808219178e-6,394.520547945205e-6,723.287671232877e-6,
                           1111.23287671233e-6,1229.58904109589e-6],
                           1111.23287671233e-6,1229.58904109589e-6],
                     time:[60,600,1200,2400,3600]}];
                     time:[60,600,1200,2400,3600]}];
 +
                  [{constants:{Vc:0.004545455,Vs:(1-0.004545455)},AsT:0.467154987e-6,
 +
                    AsinT:[10.49961532e-6,16.62049351e-6,19.44416512e-6,23.07460003e-6,
 +
                            29.40009211e-6,32.06299566e-6],
 +
                    time:[1.127*60,4.993*60,9.986*60,20.159*60,30.181*60,60.035*60]}]; 
var varsToMutate = ['v5K5','K5'];
var varsToMutate = ['v5K5','K5'];

Revision as of 10:38, 30 September 2009

Igemhomelogo.png


TODO: Talk about the devices we have and in what way we want to characterize them.

Uptake measurements

Sampling scheme
Time (min)
0 10 20 40 60
As(III)exT(0)
(µM)
0 x
10 x x x x x
20 x
50 x
100 x

To efficiently look at both time and concentration dependent processes we took samples as in the table on the right. Below we list all results, which have been used for fitting all necessary parameters.

TODO: List results. Take conversion from nmol/mg and mg/ml to µM and Vc/Vs into account.

best cur gradient solved
v5/K5
v5
K5
E