Team:Groningen/Modelling/Characterization

From 2009.igem.org

(Difference between revisions)
m (Rounded sample times.)
m (The concentrations of the second experiment should also be off by a factor of 0.3, fixed that.)
Line 145: Line 145:
                     time:[60,600,1200,2400,3600]},
                     time:[60,600,1200,2400,3600]},
                   {constants:{Vc:(0.004545455/0.3),Vs:(1-(0.004545455/0.3)),pro:0,ars2T:0},AsT:0.467154987e-6,
                   {constants:{Vc:(0.004545455/0.3),Vs:(1-(0.004545455/0.3)),pro:0,ars2T:0},AsT:0.467154987e-6,
-
                     AsinT:[10.49961532e-6,16.62049351e-6,19.44416512e-6,23.07460003e-6,
+
                     AsinT:[10.49961532e-6*0.3,16.62049351e-6*0.3,19.44416512e-6*0.3,23.07460003e-6*0.3,
-
                             29.40009211e-6,32.06299566e-6],
+
                             29.40009211e-6*0.3,32.06299566e-6*0.3],
                     time:[60,300,600,1200,1800,3600]
                     time:[60,300,600,1200,1800,3600]
                       /*[1.127*60,4.993*60,9.986*60,20.159*60,30.181*60,60.035*60]*/}];   
                       /*[1.127*60,4.993*60,9.986*60,20.159*60,30.181*60,60.035*60]*/}];   

Revision as of 16:03, 2 October 2009

Igemhomelogo.png


TODO: Talk about the devices we have and in what way we want to characterize them.

Uptake measurements

Sampling scheme
Time (min)
0 10 20 40 60
As(III)exT(0)
(µM)
0 x
10 x x x x x
20 x
50 x
100 x

To efficiently look at both time and concentration dependent processes we took samples as in the table on the right. Below we list all results, which have been used for fitting all necessary parameters.

TODO: List results. Take conversion from nmol/mg and mg/ml to µM and Vc/Vs into account.

best cur gradient solved
v5/K5
v5
K5
k8/K7
k8
K7
tauBbeta4
tauB
beta4
tauR
beta1
E