Team:Groningen/Notebook/14 September 2009
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(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== www.sigmaaldrich.com '''Plasmid isolation''' Plasmid iso...)
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(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== ===GVP Cluster=== www.sigmaaldrich.com '''Plasmid isolation''' Plasmid iso...)
Newer edit →
Revision as of 07:33, 14 September 2009
Wet
GVP Cluster
Plasmid isolation
Plasmid isolation was performed on the pellets of E.coli TOP10 plasmids (made on saterday and stored in -20box) with the "Sygma-Aldrich™ GenElute™ Plasmid Miniprep Kit".
- From each tube 5mL of culture was collected in a 2.0mL cup, and the cells were pelleted by centrifugation for 1 min. at max. speed and the supernatant discarded.
- Plasmids were eluted with 30μL MQ and stored in the fridge
Concentrations
Plasmid | Conc. ng/μL | 260/280 | 260/230 | -20 box (michael | Restriction Control |
pSB2K3-pZntR-GVP (12L, no.1) | x | x | x | x | Yes (EcoRI/PstI) |
pSB2K3-pZntR-GVP (12L, no.2) | x | x | x | x | Yes (EcoRI/PstI) |
pSB2K3-pCueO-GVP (12L, no.1) | x | x | x | x | Yes (EcoRI/PstI) |
pSB2K3-pCueO-GVP (12L, no.2) | x | x | x | x | Yes (EcoRI/PstI) |
Transporters
Metal Accumulation
Vectors
Dry
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