Team:Groningen/Notebook/15 July 2009
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Wet
GVP Cluster
Restriction for Assembly
The vector containing GVP cluster was cut with PstI and XbaI to create correct ends for insert in promotor vector.
- 10μL MQ
- 6μL plasmid in MQ (2μg)
- 2μL Fast digest buffer
- 1μL PstI fast digest enzyme
- 1μL XbaI fast digest enzyme
The vector containing promotor BBa_J23100 was cut with SpeI and PstI.
- 4μL MQ
- 10μL plasmid in MQ
- 2μL Fast digest buffer
- 1μL SpeI fast digest enzyme
- 1μL PstI fast digest enzyme
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