Team:Groningen/Notebook/17 July 2009
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All colonies (transformants vector + promoter ligated with gvp) and o/n culture became red, so probably RFP is still in there... | All colonies (transformants vector + promoter ligated with gvp) and o/n culture became red, so probably RFP is still in there... | ||
- | What might be the problem? The vector with promoter self-ligated due to uncomplete digestion [https://2009.igem.org/Team:Groningen/Notebook/15_July_2009 15 July 2009] leading to the following fragments: | + | What might be the problem? The vector with promoter self-ligated due to uncomplete digestion (done [https://2009.igem.org/Team:Groningen/Notebook/15_July_2009 15 July 2009]) leading to the following fragments: |
*vector+RFP linear | *vector+RFP linear | ||
*vector | *vector |
Revision as of 08:43, 17 July 2009
Wet
GVP Cluster
Discussion:
All colonies (transformants vector + promoter ligated with gvp) and o/n culture became red, so probably RFP is still in there... What might be the problem? The vector with promoter self-ligated due to uncomplete digestion (done 15 July 2009) leading to the following fragments:
- vector+RFP linear
- vector
- RFP
When the upper fragment (~2kb) is contaminated with linear vector this would lead high efficiency self-ligation instead of ligating gvp with the emtpy vector. -->Therefore the restriction/purification/ligation will be redone and selection of non-red colonies will be made! -->Also plasmid from the o/n cultures will be purified and analysed by restriction analysis.
Transporters
Metal Accumulation
Vectors
Dry
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