Team:Groningen/Notebook/17 July 2009
From 2009.igem.org
(Difference between revisions)
(→Transporters) |
(→Transporters) |
||
Line 26: | Line 26: | ||
| align="center" style="background:#f0f0f0;"|'''PCR 16-07''' | | align="center" style="background:#f0f0f0;"|'''PCR 16-07''' | ||
| align="center" style="background:#f0f0f0;"|'''Sample names''' | | align="center" style="background:#f0f0f0;"|'''Sample names''' | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
|- | |- | ||
| Component||B1||B2||C1||C2||A1||A2||E1||E2||F1||F2 | | Component||B1||B2||C1||C2||A1||A2||E1||E2||F1||F2 |
Revision as of 10:37, 17 July 2009
Wet
GVP Cluster
Discussion:
All colonies (transformants vector + promoter ligated with gvp) and o/n culture became red, so probably RFP is still in there... What might be the problem? The vector with promoter self-ligated due to uncomplete digestion (done 15 July 2009) leading to the following fragments:
- vector+RFP linear
- vector
- RFP
When the upper fragment (~2kb) is contaminated with linear vector this would lead high efficiency self-ligation instead of ligating gvp with the emtpy vector.
- →TODO Therefore the restriction/purification/ligation will be redone and selection of non-red colonies will be made!
- →DONE Also plasmid from the o/n cultures will be purified and analysed by restriction analysis.
- Used Sigma Plasmid isolation kit.
- DNA concentrations:
Transporters
|
Metal Accumulation
Vectors
Dry
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|