Team:Groningen/Notebook/20 August 2009
From 2009.igem.org
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+ | Due to the mutation. restarted cloning again with PCR on pBAD plasmid with primers F2 rev | ||
===Metal Accumulation=== | ===Metal Accumulation=== |
Revision as of 15:53, 20 August 2009
Meeting with iGEM team TU delft
Presentation given in Delft: http://docs.google.com/present/edit?id=0Ac_ofk2hDJzCZGNya25wYmpfNTBoaGh6Z2NkNQ&hl=en
Wet
GVP Cluster
Trying to get a nice picture of the tubes but they are not very photogenic.
- Zn seems to work in a gradient accept for the tube without Zn.
- Ars all are floating (could be very leaky/constituitive)
- Cu shows a gradient effect
- pNL29 shows a clear floating difference
photos comming asap.
Transporters
Due to the mutation. restarted cloning again with PCR on pBAD plasmid with primers F2 rev
Metal Accumulation
Vectors
Dry
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