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Team:Groningen/Notebook/20 August 2009
From 2009.igem.org
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(New page: {{Team:Groningen/Notebook/Day/Header}} Meting with iGEM team TU delft. ==Wet== ===GVP Cluster=== ===Transporters=== ===Metal Accumulation=== ===Vectors=== ==Dry== {{Team:Groningen/...) |
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{{Team:Groningen/Notebook/Day/Header}} | {{Team:Groningen/Notebook/Day/Header}} | ||
| - | + | =Meeting iGEM teams at TU delft= | |
| - | + | [http://docs.google.com/present/edit?id=0Ac_ofk2hDJzCZGNya25wYmpfNTBoaGh6Z2NkNQ&hl=en Presentation] given in Delft. | |
==Wet== | ==Wet== | ||
===GVP Cluster=== | ===GVP Cluster=== | ||
| + | Trying to get a nice picture of the tubes but they are not very photogenic. | ||
| + | |||
| + | :Zn seems to work in a gradient accept for the tube without Zn. | ||
| + | :Ars all are floating (could be very leaky/constituitive) | ||
| + | :Cu shows a gradient effect | ||
| + | :pNL29 shows a clear floating difference | ||
| + | [[Image:Zn_IMG_3817.JPG|200px|thumb|left|increase concentration, red culture negative contole]] | ||
| + | |||
| + | [[Image:Ars_IMG_3820.JPG|200px|thumb|left|increase concentration, full floating. red culture negative contol]] | ||
| + | |||
| + | [[Image:Cu_IMG_3814.JPG|200px|thumb|left|increase concentration, red culture negative contole]] | ||
| + | |||
| + | [[Image:pNL29 IMG_3823.JPG|200px|thumb|left|Red is negtive controle, 1mm full floating and 0,1 mm same level alscontrole]] | ||
===Transporters=== | ===Transporters=== | ||
| + | |||
| + | Due to the mutation. restarted cloning again with PCR on pBAD plasmid with primers F2 rev | ||
===Metal Accumulation=== | ===Metal Accumulation=== | ||
Latest revision as of 13:00, 21 August 2009
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Meeting iGEM teams at TU delft
Presentation given in Delft.
Wet
GVP Cluster
Trying to get a nice picture of the tubes but they are not very photogenic.
- Zn seems to work in a gradient accept for the tube without Zn.
- Ars all are floating (could be very leaky/constituitive)
- Cu shows a gradient effect
- pNL29 shows a clear floating difference
Transporters
Due to the mutation. restarted cloning again with PCR on pBAD plasmid with primers F2 rev
Metal Accumulation
Vectors
Dry
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