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Team:Groningen/Notebook/21 July 2009
From 2009.igem.org
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Therefor we will redesign the cloning. The Forward primer will be modified into 2 part. which will require an additional PCR but increases the chance of getting product. | Therefor we will redesign the cloning. The Forward primer will be modified into 2 part. which will require an additional PCR but increases the chance of getting product. | ||
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===Metal Accumulation=== | ===Metal Accumulation=== | ||
Revision as of 12:16, 21 July 2009
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Wet
GVP Cluster
Transporters
HmtA
The band at ~1150 kb was cut out of the gel and used for pcr described yesterday gave poor results. No band of expected size and strong primerdimer bands Therefor we will redesign the cloning. The Forward primer will be modified into 2 part. which will require an additional PCR but increases the chance of getting product.
Metal Accumulation
Vectors
Dry
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