From 2009.igem.org
Wet
GVP Cluster
TODO Restrict GVP biobrick with XbaI/PstI and purify both vector and GVP fragment
TODO Restict GVP fragment and pMA-gvpL with MvaI/XhoI and purify 310bp. 2200bp, and 3300bp fragments
TODO Ligate the three fragments into the vector and transform E.coli DB3 cells
TODO Find out wath the problem is with RBS??
- Is it one of the restriction enzymes (SpeI/PstI) failing work
- Something wrong with biobrick in the sense that there is a red colour where none was expected (where did the RFP come from, and why is it activated so strongly)
340px
- → From left to right: 1kB ladder, Sample Steven, SpeI/PstI control with BBa_K190017, Empty Slot, GVP (6x)
- → For GVP two fragments of ~6000bp and ~3000bp are expected, and for BBa_K190017 fragments of ~900bp and ~2000bp are expected.
Transporters
Metal Accumulation
Vectors
Dry