Team:Groningen/Notebook/2 July 2009
From 2009.igem.org
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Yesterday we did a restriction digest (.5ug digested with 1U enzyme) on pBAD-HmtA (AgeI and PstI) and on pUC19 (PvuI), and tested the BioBrick gvp construct and terminator by PCR(protocol Quality Control iGEM 2008).
Contents of the gel:
- =empty
- =1kb marker (Fermentas)
- =T1 (expected size...)
enz.. Conclusion:
- PCR products were to large, maybe one of the primers had gone bad...
Or the construct is not correct..
- Restriction fragments were ok, only PvuI did not completely cut pUC19 as there is an extra band with the size of single cut plasmid.