Team:Groningen/Notebook/4 August 2009
From 2009.igem.org
(Difference between revisions)
m (→GVP Cluster) |
(→Transporters) |
||
Line 14: | Line 14: | ||
===Transporters=== | ===Transporters=== | ||
+ | GlpF | ||
+ | |||
+ | Restriction, ligation and transformation. Done as noted at [https://2009.igem.org/Team:Groningen/Notebook/31_July_2009| 31 July] | ||
+ | . | ||
===Metal Accumulation=== | ===Metal Accumulation=== |
Revision as of 14:20, 4 August 2009
Wet
GVP Cluster
All precultures containing a plasmid with a promoter have grown to the expected density! Time for some plasmid isolation to be carried out...
- → TODO isolate plasmids from precultures
- → TODO perform a restriction on the medium promoter containing plasmids with SpeI and PstI
- → TODO purify cut vectors with miniprep (11bp fragment should be lost)
- → TODO perform a ligation between cut vector and GVP fragment
- → TODO transform E.coli TOP10 competent cells with ligation product
Transporters
GlpF
Restriction, ligation and transformation. Done as noted at 31 July .
Metal Accumulation
Vectors
Dry
|
|
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|