Team:Groningen/Notebook/9 July 2009
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JolandaWitteveen (Talk | contribs)
(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== '''Purification of HmtA (PA Q9I147 6xHis Tag)''' * SDS-gel electrophosis * Make a 12% SDS-gel * Fill gel with samples Wash 1-2 20mM imidazo...)
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(New page: {{Team:Groningen/Notebook/Day/Header}} ==Wet== '''Purification of HmtA (PA Q9I147 6xHis Tag)''' * SDS-gel electrophosis * Make a 12% SDS-gel * Fill gel with samples Wash 1-2 20mM imidazo...)
Newer edit →
Revision as of 10:19, 9 July 2009
Wet
Purification of HmtA (PA Q9I147 6xHis Tag)
- SDS-gel electrophosis
- Make a 12% SDS-gel
- Fill gel with samples Wash 1-2 20mM imidazole, Wash1-2 40mM imidazole, Elute1-3 and ladders
- Run 1h at 100V
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