Team:Paris/10 August 2009
From 2009.igem.org
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*For [https://2009.igem.org/Team:Paris/Freezer_PCR_products#P1 P1] /Freezer_PCR_products#A9 A9] | *For [https://2009.igem.org/Team:Paris/Freezer_PCR_products#P1 P1] /Freezer_PCR_products#A9 A9] | ||
- | 600/310 = 1.93 ~ 2 | + | 600/310 = 1.93 ~ 2 [mu] |
**2:98°C 10s (Not sure to modify guys!) | **2:98°C 10s (Not sure to modify guys!) | ||
**3:59°C (for A8) /65°C (for A9) 30s | **3:59°C (for A8) /65°C (for A9) 30s |
Revision as of 20:50, 10 August 2009
Contents |
NoteBook
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Brain work
edit please ^^
Lab work
PCR
Do
- For P1 /Freezer_PCR_products#A9 A9]
600/310 = 1.93 ~ 2 [mu]
- 2:98°C 10s (Not sure to modify guys!)
- 3:59°C (for A8) /65°C (for A9) 30s
- 4:72°C 8s
- 5:72°C 10min
- 6: 4°C ~
Purification
purification with promega kits gel purification -> Very good
To do list
Matricule | TODO |
Luc | |
Romain | |
Charlotte | |
Stoff | |
Chris | |
Lisa | |
Caroline | |
Souf | |
Vicard | |
Pierre | |
Sylvain | |
Guillaume |