Team:Paris/10 August 2009

From 2009.igem.org

(Difference between revisions)
(→Lab work)
(→Lab work)
Line 119: Line 119:
<center>[[Image:Digestion060809.png]][[Image:Wiki_gel_purif_070809_P15P16P17.png]]</center>
<center>[[Image:Digestion060809.png]][[Image:Wiki_gel_purif_070809_P15P16P17.png]]</center>
 +
 +
*legend to edit
 +
 +
<center>[[Image:Wiki_gel_purif_070809_P18P19.png]][[Image:Wikidigestion060809.png]]</center>
 +
 +
</div>
</div>

Revision as of 22:04, 10 August 2009

Contents

NoteBook

June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

← Yesterday - Tomorrow →

August 10th

Brain work

edit please ^^


Lab work

PCR

  • For A8 and A9
    • 1:98°C 1min
    • 2:98°C 10s (Not sure to modify guys!)
    • 3:59°C (for A8) /65°C (for A9) 30s
    • 4:72°C 8s
    • 5:72°C 10min
    • 6: 4°C ~

Mini-Prep for Plac

Same Protocol as usual ...

Do

Dilution for Plasmids 1,2&3
Plasmid D0(000) D0(266/280) D0(320) Dilution Rule Water Addition
P1 3.10 1.72 0.004 600/310 = 1.93 ~ 2.0 µL 2.45/10.55 ~ 13 µL
P2 1.34 1.34 0.000 600/134 = 4.48 (~ 4.5 µL) 2.45/8.05 ~ 10.5 µL
P3 0.70 3.39 0.000 600/70 = 8.57 ~ 8.6 µL 2.45/3.95 ~ 6.4 µL

Gel Migration

  • legend to edit....
0708 wiki digestion P18XP.png0708 wiki digestion P18XP extract.png


  • legend to edit
0708 wiki digestion P15161719.png0708 wiki digestion P15161719 extract.png


  • legend to edit
0708 wiki purif P19.png0708 wiki purif P15161718.png


  • legend to edit
0808 gel verif multiple.png0808 wiki PCR070809.png


  • legend to edit
Digestion060809.pngWiki gel purif 070809 P15P16P17.png
  • legend to edit
Wiki gel purif 070809 P18P19.pngWikidigestion060809.png


Purification

purification with promega kits gel purification -> Very good

To do list

Matricule TODO
Luc
Romain
Charlotte
Stoff
Chris
Lisa
Caroline
Souf
Vicard
Pierre
Sylvain
Guillaume


← Yesterday - Tomorrow →

Retrieved from "http://2009.igem.org/Team:Paris/10_August_2009"