Team:Paris/10 August 2009

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Revision as of 21:41, 10 August 2009 by PierreE (Talk | contribs)

Contents

NoteBook

June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

← Yesterday - Tomorrow →

August 10th

Brain work

edit please ^^


Lab work

PCR

  • For A8 and A9
    • 1:98°C 1min
    • 2:98°C 10s (Not sure to modify guys!)
    • 3:59°C (for A8) /65°C (for A9) 30s
    • 4:72°C 8s
    • 5:72°C 10min
    • 6: 4°C ~

Mini-Prep for Plac

Same Protocol as usual ...

Do

Dilution for Plasmids 1,2&3
Plasmid D0(000) D0(266/280) D0(320) Dilution Rule Water Addition
P1 3.10 1.72 0.004 600/310 = 1.93 ~ 2.0 µL 2.45/10.55 ~ 13 µL
P2 1.34 1.34 0.000 600/134 = 4.48 (~ 4.5 µL) 2.45/8.05 ~ 10.5 µL
P3 0.70 3.39 0.000 600/70 = 8.57 ~ 8.6 µL 2.45/3.95 ~ 6.4 µL

Gel Migration

    • legend to edit....
KR000634.JPGA3 cut.JPG


  • legend to edit
KR000637.JPGA5 coupe.JPG


Purification

purification with promega kits gel purification -> Very good

To do list

Matricule TODO
Luc
Romain
Charlotte
Stoff
Chris
Lisa
Caroline
Souf
Vicard
Pierre
Sylvain
Guillaume


← Yesterday - Tomorrow →

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