Team:Paris/10 August 2009
From 2009.igem.org
Contents |
NoteBook
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Brain work
edit please ^^
Lab work
PCR
Mini-Prep for Plac
Same Protocol as usual ...
Do
Dilution for Plasmids 1,2&3 | ||||||||||
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Plasmid | D0(000) | D0(266/280) | D0(320) | Dilution Rule | Water Addition | |||||
P1 | 3.10 | 1.72 | 0.004 | 600/310 = 1.93 ~ 2.0 µL | 2.45/10.55 ~ 13 µL | |||||
P2 | 1.34 | 1.34 | 0.000 | 600/134 = 4.48 (~ 4.5 µL) | 2.45/8.05 ~ 10.5 µL | |||||
P3 | 0.70 | 3.39 | 0.000 | 600/70 = 8.57 ~ 8.6 µL | 2.45/3.95 ~ 6.4 µL |
Gel Migration
Purification
purification with promega kits gel purification -> Very good
To do list
Matricule | TODO |
Luc | |
Romain | |
Charlotte | |
Stoff | |
Chris | |
Lisa | |
Caroline | |
Souf | |
Vicard | |
Pierre | |
Sylvain | |
Guillaume |