Team:Paris/20 July 2009

From 2009.igem.org

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==NoteBook==
==NoteBook==
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{{Paris2009_Calendar}}
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{| style="color:#1b2c8a;background-color:#0;" cellpadding="0" cellspacing="1" border="0" bordercolor="#fff" width="100%" align="left"
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{{Paris2009_Calendar_Link|19_July_2009|21_July_2009}}
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!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=06 }}
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<center> '''July 20th''' </center>
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!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=07 }}
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!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=08 }}
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!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=09 }}
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!align="center"|{{ #calendar: title=Team:Paris |year=2009 | month=10 }}
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==Summary==
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===Brain work===
We just started lab work, but nobody knows what was done...
We just started lab work, but nobody knows what was done...
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==Wetlab==
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===Lab work===
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*1st strain tests.
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**[[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S1 S1]] DH5alpha
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**[[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S2 S2]] K12 MG 1655 delta recA::Kan
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*Préparation Kanamycin
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**10ml de Kanamycin à 100µg/µl
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<html>
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</div>
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<div id="paris_content_boxtop">
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</div>
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<div id="paris_content">
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</html>
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===To do list===
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*LB medium for [S1] and [S2]
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====Récupération à la souchotèque des souches:====
 
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*[[https://2009.igem.org/Team:Paris/Strains S1]] DH5alpha
 
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*[[https://2009.igem.org/Team:Paris/Strains S2]] K12 MG 1655 delta recA::Kan
 
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Ensemencement par frottage sur milieux LB solide
 
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Mise à 37°C. Culture overnight
 
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====Préparation de 10ml Kanamycin à 100µg/µl====
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{{Paris2009_Calendar_Link|19_July_2009|21_July_2009}}

Latest revision as of 18:54, 9 August 2009

Contents

NoteBook

June
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

← Yesterday - Tomorrow →

July 20th

Brain work

We just started lab work, but nobody knows what was done...

meeting : membrane

  • snare = cool
  • modelling of vesicles formation = not cool
  • luckily snare plasmid will be provided to our team , re-cool.

cool : 2 pas cool : 1

Mr.lloubès will probably provide us with cute Tol/pal system

cool : 3 pas cool : 1

Lab work

  • 1st strain tests.
    • [S1] DH5alpha
    • [S2] K12 MG 1655 delta recA::Kan
  • Préparation Kanamycin
    • 10ml de Kanamycin à 100µg/µl

To do list

  • LB medium for [S1] and [S2]


← Yesterday - Tomorrow →