Team:Paris/20 July 2009

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Contents

NoteBook

June
MTWTFSS
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8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

Summary

We just started lab work, but nobody knows what was done...

meeting : membrane

  • snare = cool
  • modelling of vesicles formation = not cool
  • luckily snare plasmid will be provided to our team , re-cool.

cool : 2 pas cool : 1

Mr.lloubès will probably provide us with cute Tol/pal system

cool : 3 pas cool : 1

Wetlab

Récupération à la souchotèque des souches:

  • [S1] DH5alpha
  • [S2] K12 MG 1655 delta recA::Kan

Ensemencement par frottage sur milieux LB solide Mise à 37°C. Culture overnight

====Préparation de 10ml Kanamycin à 100µg/µl [Protocol]====