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Team:Paris/21 July 2009
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==NoteBook== | ==NoteBook== | ||
| - | { | + | {{Paris2009_Calendar}} |
| - | + | {{Paris2009_Calendar_Link|20_July_2009|22_July_2009}} | |
| - | + | <center> '''July 21th''' </center> | |
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<html> | <html> | ||
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| - | + | ===Brain work=== | |
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Waiting for the return of Mr. Labs and his signature for the commisions. | Waiting for the return of Mr. Labs and his signature for the commisions. | ||
| - | + | Meeting at 14: | |
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*redistribution of the role and global progress : | *redistribution of the role and global progress : | ||
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**fecA: demanding construction but still in slow progression | **fecA: demanding construction but still in slow progression | ||
**TMG: new idea to bypass our transduction problem .... let's say that the research begin. | **TMG: new idea to bypass our transduction problem .... let's say that the research begin. | ||
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| + | <div id="paris_content_boxtop"> | ||
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| + | <div id="paris_content"> | ||
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| + | ===Lab work=== | ||
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| + | *Medium for [[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S1 S1]] DH5alpha and [[https://2009.igem.org/Team:Paris/Freezer_Strains_(Glycerol_stock)#S2 S2]] K12 MG 1655 delta recA::Kan | ||
| + | ** 5ml of LB + Clone of [S1] | ||
| + | ** 5ml of LB + 5µl of Kan 1000x + Clone of [S2] | ||
| + | Overnight at 37°C, 200rpm | ||
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| + | <html> | ||
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| + | <div id="paris_content_boxtop"> | ||
| + | </div> | ||
| + | <div id="paris_content"> | ||
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| + | ===To do list=== | ||
| + | *Glycerol stock for [S1] and [S2] | ||
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| + | {{Paris2009_Calendar_Link|20_July_2009|22_July_2009}} | ||
Latest revision as of 19:08, 9 August 2009
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Brain work
Waiting for the return of Mr. Labs and his signature for the commisions.
Meeting at 14:
- redistribution of the role and global progress :
- g3p: raiders of the lost phage ... We will maybe amplify the g3p by ourself with a phage m13.
- snare: there is still hope with the future plasmide to come.
- fecA: demanding construction but still in slow progression
- TMG: new idea to bypass our transduction problem .... let's say that the research begin.
Lab work
- Medium for [S1] DH5alpha and [S2] K12 MG 1655 delta recA::Kan
- 5ml of LB + Clone of [S1]
- 5ml of LB + 5µl of Kan 1000x + Clone of [S2]
Overnight at 37°C, 200rpm
