Team:Paris/5 August 2009

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Contents

NoteBook

June
MTWTFSS
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8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31

← Yesterday - Tomorrow →

August 5th

Brain work

edit...

Lab work

FM4-64 dying (wash then stain)
n°1 n°2 n°3 n°4
500µl cells - - -
centri 4000rpm 3min - - -
resuspension 500µl MgSO4 - - -
1µl de colorant 1µl de colorant 2µl 2µl
incubation 30min 37°C incubation 60min 37°C incubation 30min 37°C incubation 60min 37°C
depot sur lame - - -
nothing all cell fluo so much dye so much dye
FM4-64 dying (stain then wash)
n°5 n°6 n°9 n°10 n°13 n°14
500µl cells - - - 500µl MgSO4 500µl Medium
1µl de colorant 1µl de colorant 1µl 1µl 1µl 1µl
incubation 30min 37°C incubation 60min 37°C incubation 30min 37°C incubation 60min 37°C incubation 60min 37°C incubation 60min 37°C
centri 4000rpm 3min - - -
resuspension 500µl MgSO4 resuspension 500µl MgSO4 + filtrage + filtrage
depot sur lame - - - - -
nothing some cell fluo noise noise noise noise



100bp.png1kb.gifPCR 050809.png

To do list

Matricule TODO
Luc bacteria coloration microscope protocol
Romain Look for his feet
Charlotte bibliography on SNAREs/Autotransporters/Jun and Fos complex
Stoff merge algo protocol/ Wiki
Chris modeling: TeTR
Lisa WTF !!!
Caroline bacteria coloration microscope protocol
Souf if(!oligo){return Wiki;} return PCR;
Vicard Lab : Gel / purification / insers
Pierre Alice / Tol/Pal modelisation / geophysics
Sylvain if(!oligo){return Maltose;} return PCR;
Guillaume Miniprep for P(1/2/3/4/5/7/8) / New P7 digestion? / Glycerol Stock (DH5α/Kaio strains)