Team:Paris/27 August 2009
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gel purif using promega kit | gel purif using promega kit | ||
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+ | </div> | ||
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+ | <div class="romain"> | ||
+ | PCR | ||
+ | </div> | ||
+ | <div class="experience"> | ||
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+ | <u>'''''Pcr of''''':</u> | ||
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+ | FecR part A & B | ||
+ | Tg3p | ||
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+ | </div> | ||
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+ | <div class="romain"> | ||
+ | purification on column | ||
+ | </div> | ||
+ | <div class="experience"> | ||
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+ | <u>'''''Purif of''''':</u> | ||
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+ | OmpA linker (SacI/P) | ||
+ | FecI (X/P) | ||
+ | pBad (S/P) | ||
</div> | </div> |
Revision as of 13:33, 27 August 2009
Contents |
NoteBook
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Brain work
edit please ^^
Lab work
PCR on OmpA signal/g3p colony
Total volume = 25ul
2XSuper mix : 12,5 ul
Diluted bacteria : 5 ul
VF2 (10 uM) : 0,5 uL
VR (10uL) : 0,5 uL
H2O : 6,5 uL
gel purification
gel purif using promega kit
PCR
Pcr of:
FecR part A & B Tg3p
purification on column
Purif of:
OmpA linker (SacI/P) FecI (X/P) pBad (S/P)
To do list
edit please ^^