Team:Paris/27 August 2009
From 2009.igem.org
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(→Lab work) |
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<u>'''''Pcr of''''':</u> | <u>'''''Pcr of''''':</u> | ||
- | FecR part A & B | + | *FecR part A & B |
- | Tg3p | + | *Tg3p |
Line 92: | Line 92: | ||
<u>'''''Purif of''''':</u> | <u>'''''Purif of''''':</u> | ||
- | OmpA linker (SacI/P) | + | *OmpA linker (SacI/P) |
- | FecI (X/P) | + | *FecI (X/P) |
- | pBad (S/P) | + | *pBad (S/P) |
Revision as of 13:34, 27 August 2009
Contents |
NoteBook
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Brain work
edit please ^^
Lab work
PCR on OmpA signal/g3p colony
Total volume = 25ul
2XSuper mix : 12,5 ul
Diluted bacteria : 5 ul
VF2 (10 uM) : 0,5 uL
VR (10uL) : 0,5 uL
H2O : 6,5 uL
gel purification
gel purif using promega kit
PCR
Pcr of:
- FecR part A & B
- Tg3p
purification on column
Purif of:
- OmpA linker (SacI/P)
- FecI (X/P)
- pBad (S/P)
To do list
edit please ^^