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From 2009.igem.org
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- 11:19, 21 October 2009 (diff | hist) File:BLPresult1.png (uploaded a new version of "Image:BLPresult1.png") (top)
- 11:18, 21 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 22:50, 20 October 2009 (diff | hist) File:BLPResult2.png (uploaded a new version of "Image:BLPResult2.png") (top)
- 22:49, 20 October 2009 (diff | hist) Team:KULeuven/Wetlab/Vanillin Production (→Steps) (top)
- 22:49, 20 October 2009 (diff | hist) Team:KULeuven/Wetlab/Vanillin Production (→Steps)
- 22:48, 20 October 2009 (diff | hist) Team:KULeuven/Wetlab/Vanillin Production (→Steps)
- 22:35, 20 October 2009 (diff | hist) Team:KULeuven/Wetlab/Vanillin Production (→Goal)
- 12:20, 20 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 12:19, 20 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 12:18, 20 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 12:18, 20 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 12:17, 20 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 23:28, 19 October 2009 (diff | hist) Template:Team:KULeuven/6 October 2009/VanillinProduction (top)
- 23:27, 19 October 2009 (diff | hist) N Template:Team:KULeuven/12 October 2009/VanillinProduction (New page: * Brought the many many tubes from the vanilla experiment to HPLC measurements) (top)
- 23:25, 19 October 2009 (diff | hist) N Template:Team:KULeuven/18 October 2009/VanillinProduction (New page: * Vanilla experiment take 3! ** Transfered 1:100 to new LB cultures containing tyrosine or ferrulic acid or vanillin *** All had a concentration of 5 mg/L, vanillin was used to determine i...) (top)
- 23:20, 19 October 2009 (diff | hist) N Template:Team:KULeuven/17 October 2009/VanillinProduction (New page: * Last vanilla experiment showed unclear results, repeat the experiment but now wait a longer time to see if anything happens * Liquid cultures from PEFT, EFT, PAMSCOT and SAMS) (top)
- 23:19, 19 October 2009 (diff | hist) Template:Team:KULeuven/10 October 2009/VanillinProduction (top)
- 23:18, 19 October 2009 (diff | hist) N Template:Team:KULeuven/10 October 2009/VanillinProduction (New page: * Vanilla experiment take 2 ** From the grown cultures (EFT, PEFT, PAMSCOT, SAMS) transfer 1:100 to a new LB medium ** Add 50 mg/L Tyrosine to 1 falcon and 5 mg/L to another test tube ** ...)
- 22:57, 19 October 2009 (diff | hist) N Template:Team:KULeuven/7 October 2009/VanillinProduction (New page: * Attempts to start another vanilla experiment failed due to lack of materials ** gathered materials and next attempt will be saturday) (top)
- 22:56, 19 October 2009 (diff | hist) N Template:Team:KULeuven/9 October 2009/VanillinProduction (New page: * Liquid cultures (for sending DNA to the registry) made from: ** EF ** EFT ** PEFT ** SAMS ** PAMS ** PAMSCO ** PAMSCOT **SAM5 **SAM8 **Ech **Fcs *Liquid cultures for the next vanilla exp...) (top)
- 22:52, 19 October 2009 (diff | hist) N Template:Team:KULeuven/6 October 2009/VanillinProduction (New page: * No colonies on both plates ** best to give up on PAMSCOEFT ligation at this point since the end is nearing)
- 22:52, 19 October 2009 (diff | hist) N Template:Team:KULeuven/5 October 2009/VanillinProduction (New page: * Double Electroporation of PAMSCOEFT (used extra high volume of ligation mixture, 5 μl instead of 2-3 μl)) (top)
- 22:51, 19 October 2009 (diff | hist) N Template:Team:KULeuven/3 October 2009/VanillinProduction (New page: * Miniprep of EFT and PAMSCO * Restriction digest of 4 EFT overnight * Restriction digest of 4 PAMSCO overnight) (top)
- 22:50, 19 October 2009 (diff | hist) N Template:Team:KULeuven/2 October 2009/VanillinProduction (New page: * Reattempt to ligate PAMSCOEFT ** made liquid cultures of PAMSCO ** made liquid cultures of EFT) (top)
- 22:49, 19 October 2009 (diff | hist) N Template:Team:KULeuven/4 October 2009/VanillinProduction (New page: * Gel electrophorese of EFT and PAMSCO * Gel extraction of EFT and PAMSCO {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | EFT || 1,5 || -7,...) (top)
- 22:46, 19 October 2009 (diff | hist) N Template:Team:KULeuven/5 october 2009/VanillinProduction (New page: * Double Electroporation of PAMSCOEFT (used extra high volume of ligation mixture, 5 μl instead of 2-3 μl)) (top)
- 22:41, 19 October 2009 (diff | hist) Template:Team:KULeuven/4 october 2009/VanillinProduction (top)
- 22:41, 19 October 2009 (diff | hist) N Template:Team:KULeuven/4 october 2009/VanillinProduction (New page: * Gel electrophorese of EFT and PAMSCO * Gel extraction of EFT and PAMSCO {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | EFT || 1,5 || -7,...)
- 22:33, 19 October 2009 (diff | hist) Template:Team:KULeuven/3 october 2009/VanillinProduction (top)
- 22:30, 19 October 2009 (diff | hist) N Template:Team:KULeuven/2 october 2009/VanillinProduction (New page: * Reattempt to ligate PAMSCOEFT ** made liquid cultures of PAMSCO ** made liquid cultures of EFT) (top)
- 22:30, 19 October 2009 (diff | hist) N Template:Team:KULeuven/3 october 2009/VanillinProduction (New page: * Miniprep of EFT and PAMSCO * Restriction digest of EFT overnight * Restriction digest of PAMSCO overnight)
- 22:28, 19 October 2009 (diff | hist) N Template:Team:KULeuven/29 September 2009/VanillinProduction (New page: * Nanodrop of the six cultures of PAMSCOEFT {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | PAMSCOEFT1 || 147,4 || || |- align="center" | ...) (top)
- 22:25, 19 October 2009 (diff | hist) Template:Team:KULeuven/28 September 2009/VanillinProduction (top)
- 22:24, 19 October 2009 (diff | hist) Template:Team:KULeuven/26 September 2009/VanillinProduction (top)
- 22:23, 19 October 2009 (diff | hist) N Template:Team:KULeuven/27 September 2009/VanillinProduction (New page: * Made liquid cultures of the 4 transfered cultures of PAMSCOT * Made liquid cultures of the 4 transfered cultures of PAMSCOEFT) (top)
- 22:23, 19 October 2009 (diff | hist) N Template:Team:KULeuven/26 September 2009/VanillinProduction (New page: * Transfered 4 colonies from the electroporation of PAMSCO to a new plate * Transfered 4 colonies from the electroporation of PAMSCOT to a new plate)
- 22:22, 19 October 2009 (diff | hist) Template:Team:KULeuven/28 September 2009/VanillinProduction
- 22:21, 19 October 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Production (top)
- 22:20, 19 October 2009 (diff | hist) N Template:Team:KULeuven/28 September 2009/VanillinProduction (New page: * Miniprep of PEFT, PAMSCO electroporation, PAMSCOEFT electroporation {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | PEFT1 || 240,3 || ||...)
- 21:51, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 21:43, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 21:42, 19 October 2009 (diff | hist) N File:BLPResult2.png
- 21:39, 19 October 2009 (diff | hist) File:BLPresult1.png (uploaded a new version of "Image:BLPresult1.png")
- 21:38, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 21:36, 19 October 2009 (diff | hist) N File:BLPresult1.png
- 21:07, 19 October 2009 (diff | hist) N Template:Team:KULeuven/25 September 2009/VanillinProduction (New page: * Electroporation of PAMSCOT * Electroporation of PAMSCOEFT * Miniprep EFT {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | EFT1 || 79,8 || ...) (top)
- 21:02, 19 October 2009 (diff | hist) N Template:Team:KULeuven/24 September 2009/VanillinProduction (New page: * Made motherplate of PAMSCO * restriction digest of PAMSCO with E/S * Gel extraction PAMSCO restriction digest * Restriction digest of Terminator and EFT with E/X * Ligation of PAMSCO wit...) (top)
- 20:55, 19 October 2009 (diff | hist) N Template:Team:KULeuven/22 September 2009/VanillinProduction (New page: * Made 12 liquid cultures of PEFT cells * Made 12 liquid cultures of EFT cells * Liquid cultures of 6 PAMSCO ligation cells and transfered to a plate. * Glycerol stock of EFT, PEFT and SAM...) (top)
- 20:53, 19 October 2009 (diff | hist) N Template:Team:KULeuven/23 September 2009/VanillinProduction (New page: * Miniprep of PAMS&Co {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | PAMSCO1 || 115,6 || || |- align="center" | PAMSCO2 || 102,2 || || |...) (top)
- 20:39, 19 October 2009 (diff | hist) N Template:Team:KULeuven/21 September 2009/VanillinProduction (New page: *Plating of Pams&co *Miniprepped electroporated PEFT {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | PEFT1 1 UV || 155,4 || 1,99|| |- ali...) (top)
- 15:00, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 14:49, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 14:48, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 14:44, 19 October 2009 (diff | hist) Team:KULeuven/Project Results (→Project Results)
- 10:51, 24 September 2009 (diff | hist) N Team:KULeuven/21 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 10:51, 24 September 2009 (diff | hist) N Team:KULeuven/22 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 10:50, 24 September 2009 (diff | hist) N Team:KULeuven/23 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 10:50, 24 September 2009 (diff | hist) N Team:KULeuven/24 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = Weekly mee...) (top)
- 10:49, 24 September 2009 (diff | hist) N File:IGEM 24-09.pdf (top)
- 12:10, 21 September 2009 (diff | hist) N Template:Team:KULeuven/20 September 2009/VanillinProduction (New page: * Transfered colonies to liquid culture, there were no more Ap plates so only liquid cultures. ** from PEFT 1 UV 8 colonies ** from PEFT 2 UV 4 colonies ** From PEFT 1 4 colonies ** From P...) (top)
- 12:08, 21 September 2009 (diff | hist) N Template:Team:KULeuven/19 September 2009/VanillinProduction (New page: * Miniprep PAMS * Restriction digest PAMS with S/P * Restriction digest COMT 13, 11, 21, 31 , 41, 42 with X/P * Ligation PAMS&CO (Promotor, Sam5, Sam8, ComT) ** Again one part from gel ext...) (top)
- 12:06, 21 September 2009 (diff | hist) N Template:Team:KULeuven/18 September 2009/VanillinProduction (New page: * Miniprep EFT * Restriction digest EFT with X/P * Gel electrophoresis EFT for 1,5 hour, because the difference between insert (2600bp) and vector (3100bp) is small. ** EFT is present in ...) (top)
- 12:03, 21 September 2009 (diff | hist) N Template:Team:KULeuven/17 September 2009/VanillinProduction (New page: * EFT Ligation transfered colonies to a new plate and to liquid culture * Electroporation of PAMS (Promotor, Sam5, Sam8)) (top)
- 12:01, 21 September 2009 (diff | hist) Team:KULeuven/17 September 2009 (top)
- 12:00, 21 September 2009 (diff | hist) N File:IGEM 17-09.pdf (top)
- 13:45, 19 September 2009 (diff | hist) N Template:Team:KULeuven/16 September 2009/VanillinProduction (New page: * Restrictions from yesterday were put on gel (COMT2, 3, SAMS, Promotor) ** SAMS and promotor( J23110??) were gel purified {| border ="1" align="center" !| part || concentration || 260/28...) (top)
- 13:35, 19 September 2009 (diff | hist) N Template:Team:KULeuven/15 September 2009/VanillinProduction (New page: * Restriction SAMS1 1 X/P, {{Kulpart|BBa_J23109}} S/P, EF1 and EF2 E/S, COMT4 2 and COMT1 1 E/X ** SAMS and EF show expected results, promotor shows the same result as yesterday (1 part of...) (top)
- 13:29, 19 September 2009 (diff | hist) Template:Team:KULeuven/14 September 2009/VanillinProduction (top)
- 13:28, 19 September 2009 (diff | hist) N Template:Team:KULeuven/14 September 2009/VanillinProduction (New page: * Miniprep of COMT {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | COMT1 1 || 63,5 || 1,98 || |- align="center" | COMT1 2 || 99,4 || 1,95 ...)
- 13:17, 19 September 2009 (diff | hist) N Template:Team:KULeuven/13 September 2009/VanillinProduction (New page: * Transferred the 4 COMT colonies to liquid cultures) (top)
- 13:16, 19 September 2009 (diff | hist) N Team:KULeuven/20 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 13:16, 19 September 2009 (diff | hist) N Team:KULeuven/19 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 13:16, 19 September 2009 (diff | hist) N Team:KULeuven/18 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 13:16, 19 September 2009 (diff | hist) N Team:KULeuven/17 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...)
- 13:15, 19 September 2009 (diff | hist) N Team:KULeuven/16 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 13:15, 19 September 2009 (diff | hist) N Team:KULeuven/15 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 13:15, 19 September 2009 (diff | hist) N Template:Team:KULeuven/12 September 2009/VanillinProduction (New page: * COMT transfered from old plate to new * No growth on EFT electroporated plates :() (top)
- 13:14, 19 September 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Production
- 13:13, 19 September 2009 (diff | hist) N Team:KULeuven/14 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 13:13, 19 September 2009 (diff | hist) N Team:KULeuven/13 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 13:13, 19 September 2009 (diff | hist) N Team:KULeuven/12 September 2009 (New page: {{Team:KULeuven/Common2/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common2/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don...) (top)
- 13:11, 19 September 2009 (diff | hist) N Template:Team:KULeuven/11 September 2009/VanillinProduction (New page: * Miniprep SAMS {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |- align="center" | SAMS1 1 || 118,2 || || |- align="center" | SAMS1 2 || 105,2 || || |- ali...) (top)
- 11:20, 14 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis) (top)
- 16:48, 10 September 2009 (diff | hist) File:IGEM 10-09.pdf (top)
- 16:48, 10 September 2009 (diff | hist) Team:KULeuven/10 September 2009 (top)
- 16:47, 10 September 2009 (diff | hist) N File:IGEM 10-09.pdf
- 16:45, 10 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 16:39, 10 September 2009 (diff | hist) N Template:Team:KULeuven/10 September 2009/VanillinProduction (New page: * Gel electrophoresis of TER restriction ** The vector for the TER is Psb1AK3 and has 3100bp, with insert is ±3200 bp ** Gel extraction {| border ="1" align="center" !| part || concentra...)
- 16:08, 10 September 2009 (diff | hist) Template:Team:KULeuven/7 September 2009/VanillinProduction
- 14:40, 9 September 2009 (diff | hist) Template:Team:KULeuven/9 September 2009/VanillinProduction
- 14:19, 9 September 2009 (diff | hist) N Team:KULeuven/11 September 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't...)
- 14:18, 9 September 2009 (diff | hist) N Team:KULeuven/10 September 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't...)
- 14:18, 9 September 2009 (diff | hist) N Team:KULeuven/9 September 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't...)
- 14:18, 9 September 2009 (diff | hist) N Team:KULeuven/8 September 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't...)
- 14:18, 9 September 2009 (diff | hist) N Team:KULeuven/7 September 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't...)
- 14:17, 9 September 2009 (diff | hist) N Team:KULeuven/6 September 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Notebook}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't...)
- 14:06, 9 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 13:58, 9 September 2009 (diff | hist) N Template:Team:KULeuven/9 September 2009/VanillinProduction (New page: * Restriction digest on Terminator * Growth on SAMS plates (woohoo!) * Transfered 4 colonies from the SAMS plates (SAMS1, SAMS1 pellet, SAMS2, SAMS2 pellet) to a new plate)
- 13:32, 9 September 2009 (diff | hist) N Template:Team:KULeuven/8 September 2009/VanillinProduction (New page: * Restriction digest on EF1, EF2, EF3 and EF4 ** Gel electrophorese of digestion, result: EF1 and EF3 appear to have an insert. The insert of EF4 is too short, beteen 1500 and 2000 bp, mig...)
- 15:13, 8 September 2009 (diff | hist) Template:Team:KULeuven/7 September 2009/VanillinProduction
- 13:12, 8 September 2009 (diff | hist) N Template:Team:KULeuven/7 September 2009/VanillinProduction (New page: * Miniprep from the four colonies of EF ligation ** Accidentally used double volume of elution buffer on EF4 {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |-...)
- 13:09, 8 September 2009 (diff | hist) Template:Team:KULeuven/6 September 2009/VanillinProduction
- 13:08, 8 September 2009 (diff | hist) N Template:Team:KULeuven/6 September 2009/VanillinProduction (New page: * Restriction digest overnight of Sam5 (1,2) and Sam8 (1,2))
- 13:08, 8 September 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Production
- 12:07, 8 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 12:03, 8 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 15:09, 4 September 2009 (diff | hist) N Template:Team:KULeuven/4 September 2009/VanillinProduction (New page: * The restriction digest from SAMS and the terminator was put on gel ** It appears there is no SAMS in between the vector, the length is approx. 1500 bp which can only be Sam5 or Sam8 * El...)
- 15:01, 4 September 2009 (diff | hist) Template:Team:KULeuven/3 September 2009/VanillinProduction
- 15:00, 4 September 2009 (diff | hist) Template:Team:KULeuven/3 September 2009/VanillinProduction
- 14:27, 4 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 14:20, 4 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 14:09, 4 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 13:59, 4 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 14:08, 3 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 14:05, 3 September 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 12:15, 2 September 2009 (diff | hist) Template:Team:KULeuven/2 September 2009/VanillinProduction
- 09:19, 2 September 2009 (diff | hist) N Template:Team:KULeuven/2 September 2009/VanillinProduction (New page: * 4 grown colonies on liquid culture)
- 09:00, 2 September 2009 (diff | hist) Template:Team:KULeuven/1 September 2009/VanillinProduction
- 11:11, 1 September 2009 (diff | hist) Template:Team:KULeuven/1 September 2009/VanillinProduction
- 11:08, 1 September 2009 (diff | hist) N Template:Team:KULeuven/1 September 2009/VanillinProduction (New page: * SAMS I B RD is used for ligation with TER II RD ** Since we want to avoid gel purification we use the direct sample from the restriction diget ** The restriction enzymes are denatured by...)
- 11:04, 1 September 2009 (diff | hist) Template:Team:KULeuven/31 August 2009/VanillinProduction
- 07:57, 1 September 2009 (diff | hist) Team:KULeuven/Wetlab/Vanillin Production (→Required)
- 14:37, 31 August 2009 (diff | hist) Template:Team:KULeuven/31 August 2009/VanillinProduction
- 14:36, 31 August 2009 (diff | hist) N Template:Team:KULeuven/31 August 2009/VanillinProduction (New page: * Electroporation of SAMS I, SAMS II AND EF II * Restriction digest of ech and fcs from saturday was tested and showed 6 signals with varying lengths for ech and 1 very large signal for fc...)
- 10:00, 31 August 2009 (diff | hist) Template:Team:KULeuven/29 August 2009/VanillinProduction
- 09:59, 31 August 2009 (diff | hist) Template:Team:KULeuven/29 August 2009/VanillinProduction
- 09:55, 31 August 2009 (diff | hist) Template:Team:KULeuven/28 August 2009/VanillinProduction
- 09:52, 31 August 2009 (diff | hist) Template:Team:KULeuven/29 August 2009/VanillinProduction
- 09:46, 31 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 09:44, 31 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 08:23, 31 August 2009 (diff | hist) Template:Team:KULeuven/29 August 2009/VanillinProduction
- 08:23, 31 August 2009 (diff | hist) N Template:Team:KULeuven/29 August 2009/VanillinProduction (New page: * Split the restriction digest in 2 parts ** 1 Part was directly used for ligation using double the mass required {| border ="1" align="center" !| Part || DNA ng || volume µl |- align="ce...)
- 08:13, 31 August 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Production
- 12:44, 27 August 2009 (diff | hist) Template:Team:KULeuven/26 August 2009/VanillinProduction
- 11:11, 27 August 2009 (diff | hist) Team:KULeuven/26 August 2009
- 11:05, 27 August 2009 (diff | hist) N Team:KULeuven/28 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 11:05, 27 August 2009 (diff | hist) N Team:KULeuven/27 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 11:05, 27 August 2009 (diff | hist) N Team:KULeuven/26 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 11:05, 27 August 2009 (diff | hist) N Team:KULeuven/25 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 11:05, 27 August 2009 (diff | hist) N Team:KULeuven/24 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 08:43, 27 August 2009 (diff | hist) Team:KULeuven/Team (→Students)
- 13:27, 26 August 2009 (diff | hist) Template:Team:KULeuven/26 August 2009/VanillinProduction
- 11:32, 26 August 2009 (diff | hist) Team:KULeuven/How it works (→Vanilla synthesis)
- 11:31, 26 August 2009 (diff | hist) Team:KULeuven/How it works (→Blue Light)
- 12:23, 25 August 2009 (diff | hist) File:Missbluevanilla.png (uploaded a new version of "Image:Missbluevanilla.png") (top)
- 11:50, 25 August 2009 (diff | hist) File:Missbluevanilla.png (uploaded a new version of "Image:Missbluevanilla.png")
- 10:42, 25 August 2009 (diff | hist) Team:KULeuven/How it works (→How it works...)
- 10:40, 25 August 2009 (diff | hist) Team:KULeuven/How it works (→How it works...)
- 10:39, 25 August 2009 (diff | hist) File:Missbluevanilla.png
- 10:39, 25 August 2009 (diff | hist) N File:Missbluevanilla.png
- 10:39, 25 August 2009 (diff | hist) Team:KULeuven/How it works (→How it works...)
- 10:38, 25 August 2009 (diff | hist) Team:KULeuven/How it works
- 10:37, 25 August 2009 (diff | hist) Team:KULeuven/How it works (→How it works...)
- 10:37, 25 August 2009 (diff | hist) Team:KULeuven/How it works (→How it works...)
- 10:36, 25 August 2009 (diff | hist) File:Mssbluevanilla.jpg (uploaded a new version of "Image:Mssbluevanilla.jpg": Reverted to version as of 09:08, 13 August 2009) (top)
- 10:36, 25 August 2009 (diff | hist) File:Missbluevanilla.tif (top)
- 10:36, 25 August 2009 (diff | hist) N File:Missbluevanilla.tif
- 10:35, 25 August 2009 (diff | hist) File:Mssbluevanilla.jpg (uploaded a new version of "Image:Mssbluevanilla.jpg": Reverted to version as of 09:08, 13 August 2009)
- 10:34, 25 August 2009 (diff | hist) File:Mssbluevanilla.jpg (uploaded a new version of "Image:Mssbluevanilla.jpg")
- 09:47, 25 August 2009 (diff | hist) Team:KULeuven/20 August 2009
- 09:47, 25 August 2009 (diff | hist) File:Presentatie 20-08.pdf (top)
- 09:47, 25 August 2009 (diff | hist) N File:Presentatie 20-08.pdf
- 09:45, 25 August 2009 (diff | hist) Team:KULeuven/13 August 2009
- 09:45, 25 August 2009 (diff | hist) File:Presentation 13-08.pdf (top)
- 09:45, 25 August 2009 (diff | hist) N File:Presentation 13-08.pdf
- 09:44, 25 August 2009 (diff | hist) Team:KULeuven/13 August 2009
- 15:24, 16 August 2009 (diff | hist) N Team:KULeuven/14 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 15:24, 16 August 2009 (diff | hist) N Team:KULeuven/13 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 15:24, 16 August 2009 (diff | hist) N Team:KULeuven/12 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 13:30, 14 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 13:24, 14 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinReceptor
- 13:23, 14 August 2009 (diff | hist) N Template:Team:KULeuven/13 August 2009/VanillinReceptor (New page: # VirB and RpoA miniprepped. Tomorow proceed with mutagenesis of RpoA # VirG redone (only TOPo was visible, 200bp) PCR from 2,3 and 1' # Α, Β, Γ, s pc purification to cre...)
- 13:15, 14 August 2009 (diff | hist) N Template:Team:KULeuven/14 August 2009/VanillinReceptor (New page: # VirG 2,3,1' on gel electrophorese #* 2 and 3 look oke, 1000bp #* 1' looks oke #* 800bp fragment + 200bp TOPO) # Innoculate 2 and 3 and grow on.... # TOPO from α and s and again fro...)
- 13:10, 14 August 2009 (diff | hist) N Template:Team:KULeuven/14 August 2009/BlueLightReceptor (New page: # Transfering of the plates with cells from the ligation. 20 colonies have been transfered to a new plate to achieve more single colonies # Plating of {{kulpart|BBa_R0040}} from -80C # Ele...)
- 13:07, 14 August 2009 (diff | hist) Template:Team:KULeuven/14 August 2009/VanillinProduction
- 12:59, 14 August 2009 (diff | hist) Template:Team:KULeuven/14 August 2009/VanillinProduction
- 12:58, 14 August 2009 (diff | hist) N Template:Team:KULeuven/14 August 2009/VanillinProduction (New page: # Gel extraction of the 4 lanes from Sam8 ({{kulpart|BBa_I742106}}) and ech ({{kulpart|BBa_I742113 }}) #* To test an additional blanc we extracted a blanc piece of gel #* Nanodrop results ...)
- 12:51, 14 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinProduction (Undo revision 46429 by JochemDeen (Talk))
- 12:50, 14 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinProduction
- 12:45, 14 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinProduction
- 12:42, 14 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinProduction (Undo revision 46408 by JochemDeen (Talk))
- 12:41, 14 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinProduction
- 12:39, 14 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinProduction
- 12:38, 14 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinProduction
- 12:36, 14 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinProduction
- 12:31, 14 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinProduction
- 12:18, 14 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin synthesis)
- 10:09, 14 August 2009 (diff | hist) Template:Team:KULeuven/12 August 2009/BlueLightReceptor
- 10:08, 14 August 2009 (diff | hist) N Template:Team:KULeuven/12 August 2009/BlueLightReceptor (New page: # Gel electrophorese of BlP cut with EcoRI and SpeI # Gel extraction of the Blp Nanodrop results {| border ="1" align="center" !| Part || concentration (ng/μl) || 260/280 λ || 2...)
- 09:50, 14 August 2009 (diff | hist) N Template:Team:KULeuven/11 August 2009/BlueLightReceptor (New page: # Restriction digest #* {{kulpart|BBa_K238013}} cut with EcoRI and SpeI, incubation for 1,5 hour # Gel electrophorese of {{kulpart|BBa_K238013}} #* The cut should be 109 bp # Restriction d...)
- 15:39, 13 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/BlueLightReceptor
- 15:38, 13 August 2009 (diff | hist) N Template:Team:KULeuven/13 August 2009/BlueLightReceptor (New page: # PCR product of 12-august of pairt {{kulpart|BBa_K238013}} # Miniprep of {{kulpart|BBa_J23101}} Nanodrop results: {| border ="1" align="center" !| Part || concentration (ng/μl) || 260...)
- 15:30, 13 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinProduction
- 14:57, 13 August 2009 (diff | hist) N Template:Team:KULeuven/13 August 2009/VanillinProduction (New page: * Miniprep on Sam8 and Ech *)
- 14:42, 12 August 2009 (diff | hist) Template:Team:KULeuven/12 August 2009/VanillinProduction
- 14:41, 12 August 2009 (diff | hist) Template:Team:KULeuven/11 August 2009/VanillinProduction
- 14:39, 12 August 2009 (diff | hist) N Template:Team:KULeuven/12 August 2009/VanillinProduction (New page: *miniprep * restriction digest on Sam5, Sam8 and Fcs ** There was no growth on Ech, thus new cultures in liquid medium * Gel electrophorese on Sam5, Sam8 and Fcs * Gel extraction on Sam5 a...)
- 09:51, 12 August 2009 (diff | hist) Team:KULeuven/Ethics (→Introduction)
- 09:50, 12 August 2009 (diff | hist) Team:KULeuven/Ethics (→Introduction)
- 14:30, 11 August 2009 (diff | hist) Template:Team:KULeuven/11 August 2009/VanillinProduction
- 14:29, 11 August 2009 (diff | hist) N Template:Team:KULeuven/11 August 2009/VanillinProduction (New page: No growth of Sam8/Sam5, fcs/ech and ComT plates. ** Probable reason is that a problem with the electrocompetent cells ** Or the concentration of Sam5/Sam8 is too low * Plan for tomorow is ...)
- 14:23, 11 August 2009 (diff | hist) N Team:KULeuven/11 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 12:34, 11 August 2009 (diff | hist) Template:Team:KULeuven/10 August 2009/VanillinProduction
- 14:53, 10 August 2009 (diff | hist) Template:Team:KULeuven/10 August 2009/VanillinProduction
- 08:29, 10 August 2009 (diff | hist) N Template:Team:KULeuven/10 August 2009/VanillinProduction (New page: * Ligated genes are electrophoresed.)
- 08:23, 10 August 2009 (diff | hist) N Team:KULeuven/10 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 08:19, 10 August 2009 (diff | hist) Template:Team:KULeuven/7 August 2009/VanillinProduction
- 08:17, 10 August 2009 (diff | hist) Template:Team:KULeuven/7 August 2009/VanillinProduction
- 09:41, 7 August 2009 (diff | hist) Template:Team:KULeuven/7 August 2009/VanillinProduction
- 09:40, 7 August 2009 (diff | hist) Template:Team:KULeuven/7 August 2009/VanillinProduction
- 09:39, 7 August 2009 (diff | hist) Template:Team:KULeuven/7 August 2009/VanillinProduction
- 09:38, 7 August 2009 (diff | hist) Template:Team:KULeuven/7 August 2009/VanillinProduction
- 09:36, 7 August 2009 (diff | hist) N Template:Team:KULeuven/7 August 2009/VanillinProduction (New page: Gel extraction of DNA from A1 (Sam5) and A2 (Sam8) results of the nanodrop: {| border ="1" align="center" !| Part || concentration (ng/&mu l) || 260/280 &lambda || 260/230 &lambda |- align...)
- 07:52, 7 August 2009 (diff | hist) File:Weekly meeting 0608.pdf (top)
- 07:51, 7 August 2009 (diff | hist) Team:KULeuven/6 August 2009
- 07:50, 7 August 2009 (diff | hist) N File:Weekly meeting 0608.pdf
- 14:34, 6 August 2009 (diff | hist) Team:KULeuven/6 August 2009
- 14:32, 6 August 2009 (diff | hist) Template:Team:KULeuven/6 August 2009/VanillinProduction
- 14:30, 6 August 2009 (diff | hist) Template:Team:KULeuven/6 August 2009/VanillinProduction
- 14:28, 6 August 2009 (diff | hist) Template:Team:KULeuven/6 August 2009/VanillinProduction
- 14:26, 6 August 2009 (diff | hist) N Template:Team:KULeuven/6 August 2009/VanillinProduction (New page: Today the purification of the gel electrophorese was done on the cells from yesterday, with the nanodrop the concentration was measured:<br> A1: 11,6 ng/ul <br> Sam5 A2: 5,5 ng/ul<br>Sam8 ...)
- 14:26, 6 August 2009 (diff | hist) Template:Team:KULeuven/5 August 2009/VanillinProduction
- 14:17, 6 August 2009 (diff | hist) Template:Team:KULeuven/5 August 2009/VanillinProduction
- 14:16, 6 August 2009 (diff | hist) Template:Team:KULeuven/5 August 2009/VanillinProduction
- 10:00, 6 August 2009 (diff | hist) N Template:Team:KULeuven/6 August 2009/BlueLightReceptor (New page: Primers for the blue light receptor have arrived) (top)
- 09:59, 6 August 2009 (diff | hist) N Template:Team:KULeuven/5 August 2009/VanillinProduction (New page: Miniprep of pairs: * RBS + Sam5 {{kulpart|K238007}} conc: 101,3 ng/ul * RBS+Sam8 {{kulpart|I742106}} conc: 95.7 ng/ul * Sam8 {{kulpart|I742142}} conc: 81,8 ng/ul * ComT {{kulpart|I742107}}...)
- 12:51, 5 August 2009 (diff | hist) N Team:KULeuven/7 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 12:51, 5 August 2009 (diff | hist) N Team:KULeuven/6 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 12:50, 5 August 2009 (diff | hist) N Team:KULeuven/5 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 12:50, 5 August 2009 (diff | hist) N Team:KULeuven/4 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 12:50, 5 August 2009 (diff | hist) N Team:KULeuven/3 August 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 12:50, 5 August 2009 (diff | hist) N Team:KULeuven/31 July 2009 (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...)
- 13:34, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Comparator (top)
- 13:34, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Receptor
- 13:33, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Production
- 13:33, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Blue Light Receptor
- 13:14, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Blue Light Receptor
- 12:29, 24 July 2009 (diff | hist) Team:KULeuven/24 July 2009 (→Progress)
- 11:39, 24 July 2009 (diff | hist) Team:KULeuven/24 July 2009 (→Progress)
- 11:38, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Production
- 11:18, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Receptor (→22 July)
- 11:16, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Receptor
- 11:14, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Comparator
- 11:13, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Blue Light Receptor
- 11:13, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Production (→14 July)
- 11:12, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Blue Light Receptor (→14 July)
- 11:12, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Production
- 11:12, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Vanillin Receptor
- 11:11, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Comparator
- 10:59, 24 July 2009 (diff | hist) Team:KULeuven/Notebook/Comparator
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