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Team:Warsaw/Calendar-Main/8 September 2009
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(New page: {{WarNotebook}} <!-- do not edit above me! --> <html> <h3><div style="text-align: center;">Cloning of the mgtc promoter into the pSB1A3 plasmid</div></h3> <h4>Kamil</h4> <br /> <p>Tasks:</...) |
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<p>Methods:</p> | <p>Methods:</p> | ||
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| - | <li>Appropriate colonies ( | + | <li>Appropriate colonies (all 5 of them) were transferred to a new plate containing ampicilin.</li> |
<li>Liquid cultures were established in 4 ml LB medium supplemented with ampicilin and incubated overnight with areation.</li> | <li>Liquid cultures were established in 4 ml LB medium supplemented with ampicilin and incubated overnight with areation.</li> | ||
</ul> | </ul> | ||
Revision as of 21:37, 8 September 2009
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Cloning of the mgtc promoter into the pSB1A3 plasmid
Kamil
Tasks:
- Colonies transfer
- Liquid cultures establishment
Methods:
- Appropriate colonies (all 5 of them) were transferred to a new plate containing ampicilin.
- Liquid cultures were established in 4 ml LB medium supplemented with ampicilin and incubated overnight with areation.
Cloning of the cro-box into the pSB1A3 plasmid
Kamil
Tasks:
- Colonies transfer
- Liquid cultures establishment
Methods:
- Appropriate colonies (all 7 of them) were transferred to a new plate containing ampicilin.
- Liquid cultures were established in 4 ml LB medium supplemented with ampicilin and incubated overnight with areation.
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