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Team:Osaka/NOTES
From 2009.igem.org
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<h2>Registry Parts</h2></div> | <h2>Registry Parts</h2></div> | ||
<div class="CollapsiblePanelContent"> | <div class="CollapsiblePanelContent"> | ||
| + | <p><b>Osaka team ALL PARTS <a href="https://2009.igem.org/Team:Osaka/Parts_%26_Devices">here</a>Parts & Devices</b><br></p> | ||
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<p><b>COLOR</b><br> | <p><b>COLOR</b><br> | ||
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<a href="http://partsregistry.org/Part:BBa_K204503">K204503</a> pT7+FliH<br> | <a href="http://partsregistry.org/Part:BBa_K204503">K204503</a> pT7+FliH<br> | ||
<a href="http://partsregistry.org/Part:BBa_K204504">K204504</a> C<sub>4</sub>HSL receiver +FliH<br> | <a href="http://partsregistry.org/Part:BBa_K204504">K204504</a> C<sub>4</sub>HSL receiver +FliH<br> | ||
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</p><br> | </p><br> | ||
Revision as of 09:12, 21 October 2009
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NOTES
Calendar
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Registry Parts
Osaka team ALL PARTS hereParts & Devices
COLOR
K204033 mOrange
placI
K204041 placI+GFP
K204042 placI+YFP
K204043 placI+CFP
K204044 placI+RFP
K204048 placI+mOrange
pcI
K204058 pcI+YFP
K204059 pcI+CFP
K204062 pcI+mOrange
ptetR
K204052 ptetR+mOrange
Carotenoid
K204064 crtE+crtB
K204065 crtI+crtY
K204068 crtZ
K204076 placI+crtE+crtB
K204077 ptetR+crtE+crtB
K204091 placI+crtE+crtB+crtI+crtY
K204095 placI+crtE+crtB+crtZ
K204096 ptetR+crtE+crtB+crtZ
gradation
K204069 cI repressor
K204086 3O6HSL receiver+RFP+cI repressor
SIGNAL (Quorum Sensing)
AI-1
K204047 LasI generater
C4HSL
K204040 RhlI (generater)
K204031 RhlR (receiver)
K204702 RhlR+GFP
3OH,C14 : 1-HSL
K204025 cinI (generater)
K204032 cinR (receiver)
3O6HSL
K204051 LuxR (receiver)
K204700 LuxR+GFP
MOTILITY
K204018 ptetR+EspE
K204501 RBS+FliH
K204502 ptetR+FliH
K204503 pT7+FliH
K204504 C4HSL receiver +FliH
Lab Note
WET Lab
Week 1 : 8/2~8/8
Brainstorming , parts planning , prepared competent cell
transformation and miniprep from MIT plate
week 2 : 8/9~8/15
start assembly parts (K204001 ~ K204006) , transformation and mini prep from MIT
Week 3 : 8/16~8/22
assembly parts (~K204015) , parts planning
Week 4 : 8/23~8/29
assembly parts (~K204021)
Week 5 : 8/30~9/5
assembly parts (~K204030)
Week 6 : 9/6~9/12
assembly parts (~K204046)
Week 7 : 9/13~9/19
assembly parts (~K204050)
Week 8 : 9/20~9/26
assembly parts (~K204056)
TEST : sensitivity of inducer and receptor
Sample : 3O6HSL , C4HSL , AI-1 , 3OH,C14:1-HSL
Result : Not confirm the fluorescence...
Week 9 : 9/27~10/3
assembly parts (~K204066 , X1~X8) , color (fluorescence) intensity check
Week 10 : 10/4~10/10
assembly parts (~K204078)
Lux receiver sensitivity test , color (fluorescence) intensity check
sequence check : generator and receiver
3O6HSL , C4HSL , AI-1 , 3OH,C14:1-HSL
Get the parts from the Chiba University Team. , Thanks chiba team
Week 11 : 10/11~10/17
assembly parts (~K2040104)
sequence check : FliH etc…
send Osaka team parts to MIT
FINISH!
Osaka team assembled about 120 parts!
Dry Lab
Week 5 : 8/30~9/5Planing model.
Week 6 : 9/6~9/12
Studying programing using Matlab.
Week 7 : 9/13~9/19
Making program in case of single coloney.
Week 8 : 9/20~9/26
Making program in case of more than two colonies
Making movie file
Searching paper written some parameter
Week 9 : 9/27~10/3
Blash up our program (~K204066 , X1~X8) , color (fluorescence) intensity check
Searching paper written some parameter
Week 10 : 10/4~10/10
Making program of color gradation
Week 11 : 10/11~10/17
Blash up our program
etc
Under construction
