Team:Warsaw/Calendar-Main/23 June 2009

From 2009.igem.org

(Difference between revisions)
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{{WarNotebook}}
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}; //koniec wewnętrznej funkcji
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req.open('GET', adresbase+nday, true);
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req.send(null);
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}
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next_fil(tokeny[tokeny.length-1].replace('/ /g','_'));
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function prev_fil(day)
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{
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var pday=prevday(day);
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document.getElementById("back_ajax").innerHTML=document.getElementById("back_ajax").innerHTML+"<br>Call: "+day;
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if (window.XMLHttpRequest) { // Mozilla, Safari, Opera ...
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  var req = new XMLHttpRequest();
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} else if (window.ActiveXObject) { // IE
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  var req = new ActiveXObject("Microsoft.XMLHTTP");
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}
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var ans;
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if (window.overrideMimeType) req.overrideMimeType('text/xml');
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req.onreadystatechange = function() { //początek wewnętrznej funkcji
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if (req.readyState == 4) {
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            if (req.status == 200) {
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                ans=req.responseText;
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                if(ans.search(eday)>0) prev_fil(pday);
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                else
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                {
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                document.getElementById("back_ajax").innerHTML=document.getElementById("back_ajax").innerHTML+"<br>Back Hit: "+pday;
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                document.getElementById("prev_fil").href=adresbase+pday;
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                document.getElementById("prev_fil").style.display = 'block';
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req.open('GET', adresbase+pday, true);
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/* ]]> */</script>
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<br/>
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<start>
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</p><p><br/>
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<h3>Components of the secretion system</h3>
<h3>Components of the secretion system</h3>
<h4>Kuba</h4>
<h4>Kuba</h4>
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<p>pVDL9 was cut with NotI</p>
<p>pVDL9 was cut with NotI</p>
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<ul>
<ul>
<ul>
<ul>

Revision as of 01:15, 24 June 2009

}; //koniec wewnętrznej funkcji

req.open('GET', adresbase+nday, true); req.send(null); }

next_fil(tokeny[tokeny.length-1].replace('/ /g','_'));


function prev_fil(day) {

var pday=prevday(day);

document.getElementById("back_ajax").innerHTML=document.getElementById("back_ajax").innerHTML+"
Call: "+day;

if (window.XMLHttpRequest) { // Mozilla, Safari, Opera ... 

  var req = new XMLHttpRequest();

} else if (window.ActiveXObject) { // IE 

  var req = new ActiveXObject("Microsoft.XMLHTTP");

} var ans; if (window.overrideMimeType) req.overrideMimeType('text/xml'); req.onreadystatechange = function() { //początek wewnętrznej funkcji if (req.readyState == 4) { 

           if (req.status == 200) {
               ans=req.responseText;
               if(ans.search(eday)>0) prev_fil(pday);
               else
               {
               document.getElementById("back_ajax").innerHTML=document.getElementById("back_ajax").innerHTML+"
Back Hit: "+pday;
               document.getElementById("prev_fil").href=adresbase+pday;
               document.getElementById("prev_fil").style.display = 'block';
               document.getElementById("prev_progress").style.display = 'none';
               }
           }

}

}; //koniec wewnętrznej funkcji

req.open('GET', adresbase+pday, true); req.send(null); }

prev_fil(tokeny[tokeny.length-1].replace('/ /g','_'));

/* ]]> */</script>
<start>

</p>


Components of the secretion system

Kuba


<p>Task:

  • Isolation and restriction analysis of the components of the hemolysin secretion system


The process

  • Plasmids pLG612-1 (containing hemolysin secretion signal) and pVDL9 (other components of the secretion system) were kindly provided by dr Guzman.

    plasmid isolation was preformed with the use of "Gel-out" kit (A&A Biotechnology)

    pLG612 was cut with EcoRI and HindIII

Retrieved from "http://2009.igem.org/Team:Warsaw/Calendar-Main/23_June_2009"