Team:Warsaw/Calendar-Main/23 June 2009

From 2009.igem.org

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<start>
 
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<h3>Components of the secretion system</h3>
<h3>Components of the secretion system</h3>
<h4>Kuba</h4>
<h4>Kuba</h4>
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<p><b>both plasmids were correctly isolated</b></p>
<p><b>both plasmids were correctly isolated</b></p>
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Revision as of 01:15, 24 June 2009

Components of the secretion system

Kuba


Task:

  • Isolation and restriction analysis of the components of the hemolysin secretion system


The process

  • Plasmids pLG612-1 (containing hemolysin secretion signal) and pVDL9 (other components of the secretion system) were kindly provided by dr Guzman.

    plasmid isolation was preformed with the use of "Gel-out" kit (A&A Biotechnology)

    pLG612 was cut with EcoRI and HindIII