Team:Warsaw/Calendar-Main/23 June 2009

From 2009.igem.org

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<li>Electrophoretic separation on 1% agarose gel</li>
<li>Electrophoretic separation on 1% agarose gel</li>
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<p>Results:</p>
<p>Results:</p>
<img src="https://2009.igem.org/Image:PLG(E%26H).jpg">
<img src="https://2009.igem.org/Image:PLG(E%26H).jpg">
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<p>pVDL9 was cut with NotI</p>
<p>pVDL9 was cut with NotI</p>
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<li>Electrophoretic separation on 1% agarose gel</li>
<li>Electrophoretic separation on 1% agarose gel</li>
</ul>
</ul>

Revision as of 01:19, 24 June 2009

Components of the secretion system

Kuba


Task:

  • Isolation and restriction analysis of the components of the hemolysin secretion system


The process

  • Plasmids pLG612-1 (containing hemolysin secretion signal) and pVDL9 (other components of the secretion system) were kindly provided by dr Guzman.

    plasmid isolation was preformed with the use of "Gel-out" kit (A&A Biotechnology)

    pLG612 was cut with EcoRI and HindIII

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